ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Electronic Resource

A New Marine Interstitial Ciliate from the Northwest Atlantic Coast Tracheloraphis primitarum Sp. N. (Ciliophora, Kinetofragminophora, Karyorelictida, Trachelocercidae) (1994)

EPSTEIN, SLAVA S.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 41 (1994), S. 0

add to mindlist on the mindlist

Details

ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: A new species of marine interstitial ciliate, Tracheloraphis primitarum sp. n., is described from intertidal sands (160-200-μm medium grain size) along the Northwest Atlantic coast. Living specimens are not transparent under incident light, 0.4-0.9 mm in length, and are characterized by an elongated body, bulbous anterior and rounded posterior regions. Cytostome is simple without a cleft on the lip. Protargol silver impregnation shows 18-29 longitudinal kineties. The glabrous zone is very narrow, 6-7 μm wide, and corresponds to the area occupied by one kinety and two interkinetal spaces. Ten to fifteen kineties terminate against the glabrous zone. Interkinetal extrusomes are present. The nuclear apparatus is a single centrally-placed nuclear group that consists of three large irregular macronuclei and 2-3 micronuclei; these are either clustered or encapsulated.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1994.tb06049.x

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

2

Electronic Resource

Epibiotic Bacteria on Several Ciliates from Marine Sediments (1998)

Epstein, Slava S. ; Bazylinski, Dennis A. ; Fowle, William H.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 45 (1998), S. 0

add to mindlist on the mindlist

Details

ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Bacterial epibionts were observed on the surface of the marine sediment ciliate Geleia fossata. Rod-shaped bacteria, from 2-10 X103 per ciliate, were universally positioned in ciliated grooves, in apparent spatial association with dikinetids. SEM and TEM examination of the ciliates confirmed that a tight affiliation exists between the epibiotic bacteria and ciliate cortex infrastructures. These observations, as well as the distinct bacterial distribution pattern over ciliate surface, suggest that there is a close epibiont/host physiological integration. Epibiotic bacteria were also observed on the surfaces of other sediment ciliates from the genera Loxophyllum, Tracheloraphis, Geleia, Paraspathidium, and Cyclidium. These findings indicate that the bacterial/protozoa associations are widespread in the marine benthic environment. The potential benefits for both epibionts and their hosts are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.1998.tb05071.x

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Electronic Resource

Interstitial Ciliates: Benthic Microaerophiles or Planktonic Anaerobes? (2003)

HAYWARD, BRETT H. ; DROSTEB, RITA ; EPSTEIN, SLAVA S.

Oxford, UK : Blackwell Publishing Ltd

The @journal of eukaryotic microbiology 50 (2003), S. 0

add to mindlist on the mindlist

Details

ISSN: 1550-7408

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: . We observed marine benthic interstitial ciliates Geleia sp. and Tracheloraphis sp. inhabiting the water column of a chemically stratified salt pond. This habitat is uncharacteristic for interstitial ciliates, yet they displayed active and abundant planktonic populations (up to 800 and 250 cells/liter, respectively) and a well-defined pattern of vertical distribution. Completely absent from the oxygenated epilimnion, they first appeared at the oxic/anoxic interface and were present throughout the anoxic hypolimnion. The data could not be explained by a passive removal (e.g. by currents) of these ciliates from their conventional habitat (soft sediments) to water column. The results suggest that 1) these ciliates favored an anoxic environment, and 2) they switched to a planktonic lifestyle as appropriate conditions (seasonal anoxia) developed in the water column. This sharply contrasts the classic view of these ciliates as specifically benthic and aerobic (albeit microaerophilic) organisms. We hypothesize that Geleia sp. and Tracheloraphis sp. can readily grow in either water column or benthos, but are typically found in sediments simply because they contain their preferred (anoxic) niche.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00148.x

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

4

Electronic Resource

Size-selective grazing of coastal bacterioplankton by natural assemblages of pigmented flagellates, colorless flagellates, and ciliates (1992)

Epstein, Slava S. ; Shiaris, Michael P.

Springer

Microbial ecology 23 (1992), S. 211-225

add to mindlist on the mindlist

Details

ISSN: 1432-184X

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Fluorescently-labelled bacteria (FLB) were used to study the feeding strategies of a natural assemblage of estuarine protozoans and to examine whether the protozoan grazing could account for the in situ size structure of the bacterioplankton. The FLB, DTAF-stained enterococci, ranging in volume from 0.01 to 0.30 × 10−1 µm3, were added to a natural planktonic assemblage at a density of 5.5% of the natural bacterioplankton. Initial densities (individuals ml−1) were as follows: total natural bacteria, 2.2 × 106; FLB, 1.2 × 105; pigmented flagellates, 300; colorless flagellates, 250; and ciliates, 30. FLB consumption rates were determined by examining the contents of protozoan food vacuoles, and the long-term effect of grazing (over a period of 100 hours) was determined by monitoring the decline in the FLB density in experimental vessels. The average consumption rates of FLB by pigmented flagellates were similar to those by flagellates that lacked chloroplasts (0.9 and 0.6 FLB protozoan−1 hour−1, respectively). The ciliates consumed bacteria at an average rate that was 17-fold higher (per cell) than flagellates, and they displayed a greater preference for larger bacteria than did the flagellates. FLB of the mid-size classes (0.025–0.100 µm3) were heavily grazed by the entire protozoan assemblage; the smallest (〈0.025 µm3) and the largest (〉0.100 µm3) FLB escaped protozoan grazing. This had a profound effect on the resulting size distribution of FLB. At the end of a 100-hour incubation, the percentage of mid-size FLB (0.025 to 0.100 µm3) decreased 2.0–2.2-fold, while the percentage of the smallest and the largest FLB increased 2.0–2.5-fold. Resultant densities of FLB were consistent with initial clearance rates determined for the protozoan groups. The grazing rates of protozoans on FLB were species-specific; whereas some species consumed FLB, others did not demonstrate bacterivory. The results suggest that protozoan grazing has a major effect on the size distribution of coastal bacterioplankton. By selectively feeding on a particular size-class of bacteria, planktonic ciliates may consume 15–90% day−1 of the standing stock of largest size classes of bacterioplankton. Thus, ciliates, which were present in low abundance in the field, could not balance the production of the entire bacterial community, but they may strongly influence the portion of the bacterial community represented by the largest bacterial class. The direct effect of flagellates (e.g., grazing) was limited to smaller bacteria.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00164097

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

5

Unknown

Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea (2009)

Edgcomb, Virginia P. ; Orsi, William D. ; Leslin, Chesley ; [et al.]

add to mindlist on the mindlist

Details

Publication Date: 2022-05-26

Description: Author Posting. © The Author(s), 2008. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Extremophiles 13 (2009): 151-167, doi:10.1007/s00792-008-0206-2.

Description: Environmental factors restrict the distribution of microbial eukaryotes but the exact boundaries for eukaryotic life are not known. Here we examine protistan communities at the extremes of salinity and osmotic pressure, and report rich assemblages inhabiting Bannock and Discovery, two deep-sea superhaline anoxic basins in the Mediterranean. Using a rRNA-based approach, we detected 1538 protistan rRNA gene sequences from water samples with total salinity ranging from 39 g/kg to 280 g/Kg, and obtained evidence that this DNA was endogenous to the extreme habitats sampled. Statistical analyses indicate that the discovered phylotypes represent only a fraction of species actually inhabiting both the brine and the brine-seawater interface, with as much as 82% of the actual richness missed by our survey. Jaccard indices (e.g., for a comparison of community membership) suggest that the brine/interface protistan communities are unique to Bannock and Discovery basins, and share little (0.8-2.8%) in species composition with overlying waters with typical marine salinity and oxygen tension. The protistan communities from the basins’ brine and brine/seawater interface appear to be particularly enriched with dinoflagellates, ciliates and other alveolates, as well as fungi, and are conspicuously poor in stramenopiles. The uniqueness and diversity of brine and brine-interface protistan communities make them promising targets for protistan discovery.

Description: This study was supported by grant grant STO414/2-4 of the Deutsche Forschungsgemeinschaft, the EuroDEEP program of the European Science Foundation under 06-EuroDEEP-FP-004 MIDDLE project and NSF-grant MCB- 0348341

Keywords: Anoxic ; Brine ; Community structure ; Deep-sea ; DHAB ; Hypersaline ; Molecular diversity ; Protists

Repository Name: Woods Hole Open Access Server

Type: Preprint

Format: application/pdf

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

PAPER (OA)

S·F·X

Fulltext

6

Unknown

Genealogical analyses of multiple loci of litostomatean ciliates (Protista, Ciliophora, Litostomatea) (2015)

Vd'acny, Peter ; Bourland, William A. ; Orsi, William D. ; [et al.]

Elsevier

add to mindlist on the mindlist

Details

Publication Date: 2022-05-26

Description: © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Phylogenetics and Evolution 66 (2012): 397-411, doi:10.1016/j.ympev.2012.06.024.

Description: The class Litostomatea is a highly diverse ciliate taxon comprising hundreds of free-living and endocommensal species. However, their traditional morphology-based classification conflicts with 18S rRNA gene phylogenies indicating (1) a deep bifurcation of the Litostomatea into Rhynchostomatia and Haptoria + Trichostomatia, and (2) body polarization and simplification of the oral apparatus as main evolutionary trends in the Litostomatea. To test whether 18S rRNA molecules provide a suitable proxy for litostomatean evolutionary history, we used eighteen new ITS1-5.8S rRNA-ITS2 region sequences from various free-living litostomatean orders. These single- and multiple-locus analyses are in agreement with previous 18S rRNA gene phylogenies, supporting that both 18S rRNA gene and ITS region sequences are effective tools for resolving phylogenetic relationships among the litostomateans. Despite insertions, deletions and mutational saturations in the ITS region, the present study shows that ITS1 and ITS2 molecules can be used to infer phylogenetic relationships not only at species level but also at higher taxonomic ranks when their secondary structure information is utilized to aid alignment.

Description: Financial support was provided by the Austrian Science Foundation (FWF Projects P-19699-B17 and P-20360-B17 to Wilhelm Foissner), the Slovak Scientific Grant Agency (VEGA Project 1/0600/11 to Peter Vd’ačný), and US NSF Grants (Projects MCB-0348341 and DEB-0816840 to Slava S. Epstein).

Keywords: Body polarization ; ITS1-5.8S rRNA-ITS2 region sequences ; Motif ; Oral simplification ; Secondary structure

Repository Name: Woods Hole Open Access Server

Type: Article

Format: application/pdf

Permalink