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  • 1
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    Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat (1983)
    Springer Nature
    Details
    Publication Date: 1983-06-01
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Springer Nature
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic relationships and isozyme variability in the Heterodera avenae complex determined by isoelectrofocusing (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isozyme variability was assessed among the principal species of the cereal cyst nematode complex to complete and enhance the information provided by classical nematode systematics, in order to clarify inter- and intraspecific relationships within this complex. Twenty populations of cereal cyst nematodes (Heterodera avenae, H. filipjevi, H. latipons and H. mani) were compared by means of five different isoenzymatic systems (esterase, malate dehydrogenase, phosphoglucoisomerase, phosphoglucomutase and superoxide dismutase) using isoelectrofocusing (IEF) on the electrophoretic separation. The results are in agreement with previous morphological and biochemical characterizations, which established genetic diversity between the Gotland strain and H. avenae and identified the Gotland strain with H. filipjevi. Populations from Israel, all included in the H. avenae group, exhibited well-defined intraspecific dissimilarity. The highest degree of polymorphism was found in the H. avenae group for all five enzymatic systems studied. The H. mani population was also included in the H. avenae group by these isozyme analyses. Malate dehydrogenase, phosphoglucoisomerase and phosphoglucomutase isozymes, fractionated for the first time by IEF in the cereal cyst nematode complex, displayed a higher level of polymorphism than using conventional electrophoresis. Isoelectric focusing has proved to be a useful tool for detecting genetic diversity within and among species of the cereal cyst nematode complex and for taxonomic purposes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2001.00543.x
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  • 3
    Electronic Resource
    Electronic Resource
    Detection and analysis of inter- and intraspecific diversity of Pratylenchus spp. using isozyme markers (2000)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 49 (2000), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The root-lesion nematodes of the genus Pratylenchus are an economically important group of plant parasitic nematodes that show high similarity among sibling species. Isozyme patterns obtained by isoelectrofocusing (IEF) were used to differentiate and establish genetic relatedness among Pratylenchus species. A total of 40 populations comprising 9 Pratylenchus species and Radopholus similis from broad host and geographic origins was examined to compare isozyme patterns of esterase (EST), hexoquinase (HK), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM) and superoxide dismutase (SOD). Of these systems, only EST, MDH, PGI and PGM were useful for differentiation of P. vulnus, P. goodeyi, P. penetrans, P. scribneri, P. thornei and R. similis populations. The greatest intraspecific diversity was found within P. coffeae based on the isozyme patterns for MDH, PGI and PGM. Intraspecific variability was also detected among R. similis populations, which showed two isozyme patterns in EST and PGI systems. Less intraspecific variation was found within the P. penetrans group. The P. goodeyi population from Cameroon differed from the other populations in this specific group in its MDH, PGI and PGM phenotypes. Highly similar banding patterns of EST, MDH and PGI activity were found among the P. scribneri populations and the one population of P. agilis. A cluster analysis of the 40 populations, generated from the four enzyme banding patterns, produced groupings that broadly matched the previous classification into specific groups, reflecting intraspecific variability in some cases. The results confirm the potential use of isozyme patterns as markers for these nematode species and their value for diagnostic application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2000.00485.x
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  • 4
    Electronic Resource
    Electronic Resource
    Transfer of a dominant gene for resistance to eyespot disease from a wild grass to hexaploid wheat (1983)
    [s.l.] : Nature Publishing Group
    Nature 303 (1983), S. 698-700 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The breeding scheme is represented in Fig. 1. A hybrid was obtained between the donor and the bridge species, which was male sterile, probably due to the lack of homology between the genome complements of its progenitors. The meiosis of this hybrid was irregular and the egg cells generated ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/303698a0
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  • 5
    Electronic Resource
    Electronic Resource
    Biochemical and cytological characterization of wheat/Aegilops ventricosa addition and transfer lines carrying chromosome 4MV (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 184-188 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Powdery mildew resistance ; Biochemical markers ; Addition and transfer lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene encoding a variant of alcohol dehydrogenase, Adh-μ, has been found to be associated with the chromosome of the Mv genome which is present in type 9 wheat/Aegilops ventricosa addition line, to which the genes for protein CM-4 and for a phosphatase variant, Aph-v, had been previously assigned. Transfer line H-93-33, which has 42 chromosomes and has been derived from the cross (Triticum turgidum x Ae. ventricosa) x T. aestivum, carries genes encoding all three biochemical markers. Linkage between these genes has been demonstrated by analysis of individual kernels of the F2 (H-93-33 x T. aestivum cv. “Almatense” H-10-15). A study of the hybrids of line H-93-33 with T. aestivum H-10-15 and with the 4DS ditelosomic line has confirmed that, as suspected, the linkage group corresponds to chromosome 4Mv from Ae. ventricosa. Additionally, it has been found that the previously reported resistance of line H-93-33 to powdery mildew (Erysiphe graminis) is also linked to the biochemical markers; this indicates that either the gene responsible for it is different from that in lines H-93-8 and H-93-35, or that a translocation between two different Mv chromosomes has occurred in line H-93-33.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266184
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of wheat/Aegilops ventricosa introgression and addition lines with respect to the Mv genome (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 197-204 
    Details
    ISSN: 1432-2242
    Keywords: Aegilops ventricosa ; DNA probes ; Introgression lines ; Addition lines ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stable wheat-Aegilops introgression lines with 42 chromosomes (H-93), derived by repeated selfing from a cross (Triticum turgidum x Aegilops ventricosa) x T. aestivum, have been characterized using the following DNA probes and isozyme markers: (1) single or low-copy DNA fragments from Ae. ventricosa; (2) known cDNA probes corresponding to α1-thionin, monomeric α-amylase inhibitor, the CM3 subunit of tetrameric α-amylase inhibitor, and sucrose synthase from wheat; (3) anonymous cDNA probes from wheat that have been mapped by Sharp et al. (1989); (4) isozyme markers corresponding to aconitase, shikimate dehydrogenase, adenylate kinase, and endopeptidase. Meiotic metaphases of appropriate hybrids involving selected H-93 lines have been investigated by the Giemsa C-banding technique. The substitution of whole chromosomes [(5A) 5Mv; (4D) 4Mv; (5D) 5Mv; (7D) 7Mv] and chromosomal segments (1Mv; 3Mv; 5Mv; 7Mv) from the Mv genome of Aegilops ventricosa has been demonstrated. The distribution of selected markers among putative wheat-Ae. ventricosa addition lines has also been investigated. The 7Mv addition has been characterized for the first time, while the identity of the previously reported 5Mv and 6Mv additions has been confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222079
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic transfer of resistance to powdery mildew and of an associated biochemical marker from Aegilops ventricosa to hexaploid wheat (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 605-608 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Erysiphe graminis f.sp. tritici ; Powdery mildew resistance ; Protein U-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Resistance to powdery mildew, caused by the fungus Erysiphe graminis f.sp. tritici, has been transferred from Aegilops ventricosa (genomes DvMv) to hexaploid wheat (Triticum aestivum, ABD). In two transfer lines, H-93-8 and H-93-35, the resistance gene was linked to a gene encoding protein U-1, whereas one line, H-93-33, was resistant but lacked the molecular marker, and another line, H-93-1, was susceptible but carried the gene for U-1, indicating that the original Mv chromosome from Ae. ventricosa, carrying the two genes, had undergone recombination with a wheat chromosome in the last two lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289201
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  • 8
    Electronic Resource
    Electronic Resource
    Resistance to the cereal cyst nematode (Heterodera avenae Woll.) transferred from the wild grassAegilops ventricosa to hexaploid wheat by a “stepping-stone” procedure (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 402-408 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Heterodera avenae ; Cyst nematode ; Resistance gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transfer of resistance toHeterodera avenae, the cereal cyst nematode (CCN), by a “stepping-stone” procedure from the wild grassAegilops ventricosa to hexaploid wheat has been demonstrated. The number of nematodes per plant was lower, and reached a plateau much earlier, in the resistant introgression line H93-8 (1–2 nematodes per plant) than in the recipient H10-15 wheat (14–16 nematodes per plant). Necrosis (hypersensitive reaction) near the nematode, little cell fusion, and few, often degraded syncytia were observed in infested H93-8 roots, while abundant, well-formed syncytia were present in the susceptible H10-15 wheat. Line H93-8 was highly resistant to the two Spanish populations tested, as well as the four French races (Fr1-Fr4), and the British pathotype Hall, but was susceptible to the Swedish pathotypes HgI and HgIII. Resistance was inherited as though determined by a single quasi-dominant factor in the F2 generations resulting from crosses of H93-8 with H10-15 and with Loros, a resistant wheat carrying the geneCre1 (syn.Ccn1). The resistance gene in H93-8 (Cre2 orCcn2) is not allelic with respect to that in Loros. RFLPs and other markers, together with the cytogenetical evidence, indicate that theCre2 gene has been integrated into a wheat chromosome without affecting its meiotic pairing ability. Introduction ofCre2 by backcrossing into a commercial wheat backgroud increases grain yield when under challenge by the nematode and is not detrimental in the absence of infestation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01184930
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  • 9
    Electronic Resource
    Electronic Resource
    A map of rye chromosome 4R with cytological and isozyme markers (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 941-946 
    Details
    ISSN: 1432-2242
    Keywords: Isozymes ; Translocation ; Cytogenetic maps ; Chromosome 4R ; Rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The progeny of two crosses between a structural heterozygote for a reciprocal translocation (4RL/5RL) and a homozygote for the standard chromosome arrangement and of four crosses between standard chromosome homozygotes were analysed in rye (Secale cereale L. cv ‘Ailés’) for the electrophoretic patterns of five different leaf and endosperm isozymes (LAP, PGM, NDH, ADH and EPER). The presence or absence of the quadrivalents at metaphase I (MI) was also tested. Loci Adh-1, Pgm-1 and Ndh-1 were located on chromosome arm 4RS, and locus Eper-1 on chromosome arm 4RL. Locus Lap-2 was located on the 4RS chromosome arm. The estimated distances among the different linked loci support the following gene order: Eper1¨ (breakpoint-centromere)¨Lap-2¨ ¨Adh-1 ¨Pgm-1¨Ndh-1. These results provide evidence for the chromosomal location of Lap-2 locus on chromosome arm 4RS in cv ‘Ailés’. A high negative interference was detected between the zones delimited by centromere and Lap-2, and Lap-2 and Pgm-1 in plants with the 4RL/5RL translocation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225788
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  • 10
    Electronic Resource
    Electronic Resource
    Biochemical markers associated with two Mv chromosomes from Aegilops ventricosa in wheat-Aegilops addition lines (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 5-10 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Aegilops ventricosa ; Addition lines ; Biochemical markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The distribution of three biochemical markers, U-1, CM-4 and Aphv-a, -b, among wheat-Aegilops addition lines carrying Mv chromosomes from Aegilops ventricosa (genomes DvMv) has been investigated. Addition lines which had been previously grouped together on the basis of common non-biochemical characters carried marker U-1, a protein component from the 2M urea extract. The added chromosome, in the appropriate genetic background, seems to confer a high level of resistance to the eyespot disease, caused by the fungus Cercosporella herpotrichoides. The other two markers were concomitantly associated with another similarly formed group of addition lines. Both CM-4, a protein component from the chloroform:methanol extract, and Aphv-a, -b, alkaline phosphate isozymes, have been previously shown to be associated with homoeologous chromosome group 4, which suggests that the added chromosome in the second group of addition lines is 4Mv.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275171
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