ISSN:
1420-9071
Keywords:
Halobacteria
;
plasmid
;
insertion sequence
;
gas vesicle
;
biotechnology
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract In our efforts to elucidate the mechanism of high-frequency mutation ofHalobacterium halobium to a gas vesicle deficient state, we discovered insertions, deletions, inversions, and complex DNA rearrangements associated with a large endogenous plasmid, pNRC100. The rearrangements are mostly IS element-mediated, and when they occur in a region of pNCRC100 containing a cluster of thirteen genes, gas vesicle mutants result. We have characterized the structure and expression of this gas vesicle protein (gvp) gene cluster and demonstrated its requirement for gas vesicle synthesis and cell flotation by genetic transformation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01955149
Permalink