ISSN:
0043-7719
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Summary An enzyme-linked immunosorbent assay (ELISA), based on polyclonal antibodies, was used to quantify didecyldimethylammonium chloride (DDAC) in industrial antisapstain treating solutions. The initial ELISA validation included: cross-reactivity with similar analytes, interference with non-related compounds, percentage of false negative and false positive determinations, and comparison of the immunoassay data with the data from titration and HPLC methods. ELISA and the titration method gave comparable results. However, higher DDAC values were obtained for industrial treating solutions measured by ELISA than by HPLC. Immunoassays may be a potential analytical tool for the quality control program of DDAC type antisapstain formulations, if specificity could be improved.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002260050080
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