Publication Date:
2000-03-04
Description:
The coupling mechanism between endoplasmic reticulum (ER) calcium ion (Ca2+) stores and plasma membrane (PM) store-operated channels (SOCs) is crucial to Ca2+ signaling but has eluded detection. SOCs may be functionally related to the TRP family of receptor-operated channels. Direct comparison of endogenous SOCs with stably expressed TRP3 channels in human embryonic kidney (HEK293) cells revealed that TRP3 channels differ in being store independent. However, condensed cortical F-actin prevented activation of both SOC and TRP3 channels, which suggests that ER-PM interactions underlie coupling of both channels. A cell-permeant inhibitor of inositol trisphosphate receptor (InsP3R) function, 2-aminoethoxydiphenyl borate, prevented both receptor-induced TRP3 activation and store-induced SOC activation. It is concluded that InsP3Rs mediate both SOC and TRP channel opening and that the InsP3R is essential for maintaining coupling between store emptying and physiological activation of SOCs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ma, H T -- Patterson, R L -- van Rossum, D B -- Birnbaumer, L -- Mikoshiba, K -- Gill, D L -- AR07592/AR/NIAMS NIH HHS/ -- HL55426/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2000 Mar 3;287(5458):1647-51.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Biology, University of Maryland, School of Medicine, Baltimore, MD 21201, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10698739" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/metabolism
;
Boron Compounds/pharmacology
;
Calcium/*metabolism
;
Calcium Channels/chemistry/*metabolism
;
*Calcium Signaling
;
Carbachol/pharmacology
;
Cell Line
;
Cell Membrane/metabolism
;
Diglycerides/metabolism/pharmacology
;
Endoplasmic Reticulum/*metabolism
;
Enzyme Inhibitors/pharmacology
;
Humans
;
Inositol 1,4,5-Trisphosphate Receptors
;
Ionomycin/pharmacology
;
Macrocyclic Compounds
;
Oxazoles/pharmacology
;
Phosphoprotein Phosphatases/antagonists & inhibitors
;
Receptors, Cytoplasmic and Nuclear/chemistry/*metabolism
;
Strontium/metabolism
;
TRPC Cation Channels
;
Thapsigargin/pharmacology
;
Transfection
;
Type C Phospholipases/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink