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  • 1
    Electronic Resource
    Electronic Resource
    Stoichiometric complexation of streptomyces subtilisin inhibitor and subtilisin (1991)
    Springer
    The protein journal 10 (1991), S. 385-389 
    Details
    ISSN: 1573-4943
    Keywords: SSI-Sbt interaction ; abnormal SDS-PAGE migration ; subtilisin unfolding ; SSI digestion ; stoichiometric complexation in SDS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Subtilisin (Sbt) andStreptomyces subtilisin inhibitor (SSI) were analyzed either alone or together using sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). With all ratios of Sbt to SSI tested, the proteins formed a stoichiometric complex, and migrated abnormally at the top of the gel. Electroblotting and amino acid sequence analysis of the complex band showed both Sbt and SSI present at approximately equal molar ratios. When excess Sbt was present, it migrated as a free but still folded form slightly above the band corresponding to the complex. When excess SSI was present, it migrated as several species with molecular weights smaller than the intact form; in fact, the sequences of some of these species indicated that they lacked different amounts of N-terminal and possibly C-terminal residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025252
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    Paper (German National Licenses)
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    Exospheric neutral hydrogen density at the nominal 10 RE subsolar point deduced from XMM‐Newton X‐ray observations (2019)
    Wiley
    Details
    Publication Date: 2019
    Description: ABSTRACT We investigate the exospheric neutral density near the subsolar magnetopause, assuming Xgse = 10RE as its typical location, to support the upcoming Solar wind – Magnetosphere – Ionosphere Link Explorer (SMILE) that will visualize the Earth's magnetosheath and cusps in soft X‐rays. Neutral hydrogen density is a key parameter that controls soft X‐ray emission and can be inversely extracted from the soft X‐ray observations. We introduce a unique method to estimate the dayside neutral density by using XMM‐Newton astrophysics X‐ray observations and OpenGGCM global magnetosphere – ionosphere MHD model. On 04 May 2003 and 16 Oct 2001, the XMM‐Newton line of sight traversed the dayside of the Earth's magnetosheath and observed strong near‐Earth soft X‐ray emission. We simulate these two events using OpenGGCM. Although the model tends to produce a spatially thicker magnetosheath than measured, modeled magnetosheath plasma fluxes match well with the in‐situ observations of Cluster and Geotail. We calculate the neutral densities every thousand seconds by comparing the modeled count rates with the XMM‐Newton rates. The densities are averaged at 39.9 ± 8.0 cm−3 and 57.6 ± 8.0 cm−3 for the 04 May 2003 and 16 Oct 2001 events, respectively. Since our MHD model tends to underestimate neutral densities due to its thicker magnetosheath, the true neutral density is likely to lie within the upper half of these error bars.
    Print ISSN: 2169-9380
    Electronic ISSN: 2169-9402
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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