ISSN:
1432-2048
Keywords:
Amino acid, atomatic (biosynthesis)
;
5-Enolpyruvylshikimate 3-phosphate synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
;
Glyphosate
;
Pisum (shikimate pathway)
;
Shikimate pathway
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract 5-Enolpyruvylshikimate 3-phosphate synthase (3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19) from shoot tissue of pea seedlings was purified to apparent homogeneity by sequential ammonium-sulphate precipitation, ion-exchange and hydrophobic-interaction chromatography and substrate elution from cellulose phosphate. Gel electrophoresis and gel-permeation chromatography showed that the purified enzyme was monomeric with molecular weight 50,000. The herbicide glyphosate was a potent inhibitor of the forward enzyme-catalyzed reaction.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00392469
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