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  • 1
    Electronic Resource
    Electronic Resource
    Molecular Aspects of Self-Incompatibility in Flowering Plants (1993)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Genetics 27 (1993), S. 257-279 
    Details
    ISSN: 0066-4197
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.ge.27.120193.001353
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  • 2
    Electronic Resource
    Electronic Resource
    Arabinogalactan-Proteins: Structure, Biosynthesis, and Function (1983)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 34 (1983), S. 47-70 
    Details
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.pp.34.060183.000403
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  • 3
    Electronic Resource
    Electronic Resource
    Developmentally controlled expression of a gene associated with self-incompatibility in Nicotiana alata (1987)
    [s.l.] : Nature Publishing Group
    Nature 326 (1987), S. 99-102 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 Diagram of compatible pollination of a mature pistil of Nicotiana alata. The secretory epidermis of the stigma, transmitting tract of the style and the epidermis of the placenta are indicated by close cross-hatching; these are the tissues which hybridize with S2 cDNA and correspond to the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/326099a0
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  • 4
    Electronic Resource
    Electronic Resource
    Digestive carbohydrases from the hepatopancreas of two Antarctic euphausiid species (Euphausia superba and E. crystallorophias) (1986)
    Springer
    Marine biology 90 (1986), S. 371-378 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hepatopancreatic extracts of Euphausia superba Dana and E. crystallorophias Holt and Tattersall collected from the Antarctic. Ocean during January 1985, are most effective in hydrolysing substrates containing (1→3)-β-glucosidic linkages. Three enzymes appear to be involved in the depolymerization of (1→3)-β-D-glucans in the euphausiid diet: (1) an exo-(1→3)-β-D-glucanase, (2) an endo-(1→3)-β-D-glucanase and (3) a β-D-glucosidase. The glucanases have a pH optimum of 5.4, a temperature optimum of 50°C and are optimally extracted in bistripropane buffer, pH 7.2. Levels of (1→3)β-D-glucanase in laboratory-cultured E. superba are inversely affected by food availability, and activities double after starvation for 12 d. The increase is due mainly to higher activities of exo-acting glucanases. α-Amylase and endo-(1→4)-β-D-glucanase (cellulase) activity are also present in the extracts in addition to glycosidase activity against a range of p-nitrophenyl substrates (α-and β-D-glocose, α- and β-D-galactose, β-D-xylose, α- and β-D-mannose). Digestive activity against several acidic polysaccharides, including the acidic mucilage polysaccharide of the ice diatom Stauroneis amphioxys, is minimal and is not induced when the polysaccharide is present in the diet of E. superba. These results indicate that some, but not all, components of the algal material in the euphausiid diet can be hydrolyzed and assimilated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428561
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  • 5
    Electronic Resource
    Electronic Resource
    Pollen-expressed S-RNases are not involved in self-incompatibility in Lycopersicon peruvianum (1999)
    Springer
    Sexual plant reproduction 12 (1999), S. 76-87 
    Details
    ISSN: 1432-2145
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Self-incompatibility in solanaceous plants is gametophytically controlled by a multiallelic S-locus. The only known S-locus product is a series of extracellular ribonucleases (the S-RNases) which are expressed in the mature style and determine its self-incompatibility phenotype. Potentially, S-RNases also determine the self-incompatibility phenotype of pollen as some solanaceous plants express this protein for a brief period during anther development. To test this, we first showed that the S3-RNase of Lycopersicon peruvianum is expressed during anther development. We then transformed L. peruvianum plants with sense and antisense versions of the S3-RNase coding region under the control of a pollen-specific promoter. Pollen from the transgenic plants accumulated S3-RNase transcripts and the S3-RNase protein was detected immunologically in the sense transgenic plants. However, neither the sense nor the antisense S3-RNase constructs altered the self-incompatibility phenotype of pollen from the transgenic plants, demonstrating the S3-RNase is not the pollen product of the S3-allele.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050175
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  • 6
    Electronic Resource
    Electronic Resource
    A style-specific 120-kDa glycoprotein enters pollen tubes of Nicotiana alata in vivo (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 75-86 
    Details
    ISSN: 1432-2145
    Keywords: Key words Hydroxyproline-rich glycoprotein ; Pistil ; Pollen tubes ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Pistils of Nicotiana alata (Link et Otto) contain an abundant, style-specific glycoprotein (120 kDa) that is rich in hydroxyproline and has both extensin-like and arabinogalactan-protein-like carbohydrate substituents. An antibody specific for the protein backbone of the glycoprotein was used to localise the glycoprotein in both unpollinated and pollinated pistils. The glycoprotein is evenly distributed in the extracellular matrix of the style transmitting tract of unpollinated pistils and, despite the presence of extensin-like carbohydrate substituents, is not associated with the walls of the transmitting tract cells. In pollinated pistils the 120-kDa glycoprotein is concentrated in the extracellular matrix adjacent to pollen tubes, and is also present in the cytoplasm and the cell walls of pollen tubes. Pollen tubes grown in vitro do not contain the 120-kDa glycoprotein unless it is added to the growth medium, suggesting that the 120-kDa glycoprotein located in pistil-grown pollen tubes is derived from the extracellular matrix of the transmitting tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050013
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  • 7
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395321
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  • 8
    Electronic Resource
    Electronic Resource
    Expression sites and developmental regulation of genes encoding (1→3,1→4)-β-glucanases in germinated barley (1988)
    Springer
    Planta 173 (1988), S. 500-508 
    Details
    ISSN: 1432-2048
    Keywords: Aleurone ; (1→3,1→4)-β-Glucanase ; Hordeum (gene regulation) ; Hybridization histochemistry ; Scutellum ; Seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Expression sites of genes encoding (1→3,1→4)-β-glucan 4-glucanohydrolase (EC 3.2.1.73) have been mapped in germinated barley grains (Hordeum vulgare L.) by hybridization histochemistry. A32P-labelled cDNA (copy DNA) probe was hybridized to cryosections of intact barley grains to localize complementary mRNAs. No mRNA encoding (1→3,1→4)-β-glucanase is detected in ungerminated grain. Expression of (1→3,1→4)-β-glucanase genes is first detected in the scutellum after 1 d and is confined to the epithelial layer. At this stage, no expression is apparent in the aleurone. After 2 d, levels of (1→3,1→4)-β-glucanase mRNA decrease in the scutellar epithelium but increase in the aleurone. In the aleurone layer, induction of (1→3,1→4)-β-glucanase gene expression, as measured by mRNA accumulation, progresses from the proximal to distal end of the grain as a front moving away from, and parallel to, the face of the scutellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00958963
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  • 9
    Electronic Resource
    Electronic Resource
    Immuno-gold localization of α-L-arabinofuranosyl residues in pollen tubes of Nicotiana alata Link et otto (1987)
    Springer
    Planta 171 (1987), S. 438-442 
    Details
    ISSN: 1432-2048
    Keywords: Arabinofuranosyl residues ; Cell wall (pollen) ; Immuno-gold localization ; Nicotiana (pollen) ; Monoclonal antibody ; Pollen tube
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for α-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall: the inner, non-fibrillar wall layer was not labelled. Cytoplasmic vesicles (0.2 μm diameter) were also labelled. The antibody may bind to an arabinan in the pollen-tube wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392290
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  • 10
    Electronic Resource
    Electronic Resource
    The nature of callose produced during self-pollination inSecale cereale (1980)
    Springer
    Planta 148 (1980), S. 498-509 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Pollination ; Secale ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response to incompatible (self) pollination in rye (Secale cereale L.) includes the rapid deposition in the germinating pollen grain and pollen tube of a substance that stains with aniline blue, resorcin blue and calcofluor, and in these respects resembles callose. This substance has been isolated and analysed by acid hydrolysis and methylation as well as specific enzyme hydrolysis. It contains a glucan component with 1,4-β-glucosidic and 1,3-β-glucosidic linkages within the same linear chains. The proportion of 1,4-to 1,3-glucosidic linkages in the preparation is 77∶9.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552666
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