ISSN:
1432-1432
Keywords:
Restriction enzyme mapping
;
DNA sequencing
;
Sequence divergence
;
Cell hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The genome of the mouse (Mus musculus) contains a family of repeated DNA sequences defined by a 1.3 kb EcoRI fragment. Resqtriction maps of ten cloned fragments from this family have been determined. The fragments were of seven different types, based on the patterns of digestion obtained with AvaII, HindIII, and TaqI restriction enzymes. These seven unique sets of sequences fell into two classes, as defined by the position of a single HindIII site. Portions of fragments from each of the two classes were sequenced. Although certain regions of the repeat were highly conserved between classes, there was more intraspecific sequence divergence among the sequenced regions than has been observed for the short interspersedAlu family of repeated sequences sin mammals. Sequences of both HindIII classes were found to be present within the mouse X chromosome; we can conclude that both classes must also be present on other mouse chromosomes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02300749
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