ISSN:
1573-5044
Keywords:
Benzyladenine
;
gelling agent
;
micropropagation
;
Ulmus pumila
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A simple and reliable two-step micropropagation system was developed for mature Siberian elm (Ulmus pumila L.). Shoot-tip cultures were initiated and multiplied on a modified Murashige and Skoog medium supplemented with 1 μM benzyladenine, B5 vitamins, solidified with 0.22% Gelrite, and subcultured weekly. Proliferated shoots 2–3 cm in length rooted successfully in sterile and non-sterile soil mix at 100% efficiency. Healthy plants were acclimatized to the ambient environment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00051592
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