Publication Date:
2011-11-18
Description:
Abstract 5013 PURPOSE: This paper was to study the reverse effect of magnetic Fe3O4 nanoparticle loading daunorubicin and bromotetrandrine(Fe3O4-MNPs/DNR/BrTet) with the conduction of alternating magnetic field in nude mice bearing K562/A02 xenografts, and to provide theoretic evidence for the clinical application of malignancies as a new treatment. METHODS: 20 BALB/c nude mice with equivalent different gender, which were age-matched (four weeks of age) and weight-matched (16-22g), were randomly divided to 5 groups. The subclone of K562/A02 cells was inoculated subcutaneously into the oxter of all athymic nude mice. Group 1 each mouse was given 0.9% normal saline 0.2ml/once; Group 2, DNR 1mg/kg and BrTet 1mg/kg; Group 3, DNR 1mg/ml and BrTet 10mg/kg; Group 4, Fe3O4-MNPs 10.5mg/kg; Group 5, Fe3O4-MNPs/DNR/BrTet 12.5mg/kg(DNR: BrTet: Fe3O4-MNPs=1:1:10.5). After being delivered in an alternating magnetic field for 40minutes, the incidence of growth characteristics of mice, and volume of tumor were observed for 14 days. At the 14th day, the mice were sacrificed by cervical dislocation to detect all tumors by weight measuring, Hematoxylin and eosin staining, Western-blot and so on. RESULTS: The average tumors' weight and volume was much less in Group 4 and 5 than others, there were significantly different (P
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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