ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The reproducibility of protein migration was studied in three electrophoretic systems: sodium dodecyl-sulfate electrophoresis on polyacrylamide gradient, isoelectric focusing on immobilized pH gradients and two-dimensional charge-size separations. The gradient gels were poured either with a two-vessel apparatus or with a set of computer-driven burettes, as described by Altland and Altland (Electrophoresis 1984, 5, 143-147). Standard deviation on band position was cut by 20-50% with the latter approach in comparison with the former. Typical results were: sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 0.6 mm (over a total length of 135 mm); immobilized pH gradients, 0.6 mm (for 179 mm length); two-dimensional separations, 0.8 mm (χ-axis), 0.6 mm (y-axis).
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150070205
Permalink