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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 20 (1973), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The history and present status of our knowledge of trypanosomated kinetoplasts are reviewed and the practical and general theoretical benefits which can result from the continued study and improved understanding of these unique organelles are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 17 (1970), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Autoradiographic technics with tritium-labeled thymidine have been used to determine G1, S, G2 and D for the kinetoplast and the nucleus of Crithidia fasciculata at 15, 25 and 32 C. The kinetoplast completes division before the nucleus at all 3 temperatures. The S phases of both organelles occur in approximate synchrony and are approximately equal in length but the nucleus begins and completes S before the kinetoplast at the 2 lower temperatures. This relationship is reversed at 32 C. Most of the effect of temperature on generation time is due to its effect on the length of S.The results are compared with similar studies on C. luciliae, Trypanosoma mega, other protozoa and tissue cells in culture. The role of the approximate synchrony of nuclear and kinetoplastic cycles in maintenance of the kinetoplastic condition is discussed and the hypothesis is proposed that this synchrony results from the sharing by nucleus and kinetoplast of the same mechanism for the production of the deoxyribonucleotides used in replication of their respective DNAs.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 5 (1958), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. It has been shown that the output of the contractile vacuole of Crithidia fasciculata increases as the concentration of solute in the external solution decreases when NaCl is the solute. The increase in output is the result of increased rate of growth as well as increased maximum size, the latter being more important in the range 0.1M–0.01M. Solutions of penta-erythritol produce the same effects as isosmolar solutions of NaCl. Body volume is not maintained constant but increases less than two-fold between 0.1M and 0.0001M. The isotonic concentration of NaCl is estimated as 0.12M. It is not possible to demonstrate any effect of concentration of the external environment on the endogenous rate of oxygen consumption. A linear relationship between output and calculated osmotic gradient across the cell membrane has been shown in the concentration range 0.1M–0.01M. Absence of this relationship, and failure of body volume to increase as predicted, in solutions more dilute than 0.01M are interpreted as indications that other processes probably become predominant in these solutions. It is concluded that the contractile vacuole of this flagellate is incapable of maintaining absolute constancy of internal osmotic pressure and body volume but prevents much of the change expected on physical grounds.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 26 (1979), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Hydroxyurea (HU) inhibits increase in cell number in cultures of Crithidia fasciculata. Complete inhibition is produced by 8 mM and higher concentrations. If HU is not removed, population growth resumes in 45–50 h: if HU is removed, partially synchronous growth occurs through 2 cycles. During HU inhibition, the rate of DNA synthesis is reduced to 1% of that in exponentially growing cultures; protein and RNA syntheses continue at slightly reduced rates. Mean cell size and protein and RNA contents per cell increase; rate of oxygen consumption per mg cell protein remains constant. The behavior of a culture upon addition of HU and upon its removal agrees with predictions based on the hypothesis that the only direct effect of HU is to block DNA synthesis. The synchrony produced by HU is judged satisfactory for investigations of kinetoplast and nuclear replication but not for biochemical characterization of other aspects of the cell cycle.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 1979-01-01
    Print ISSN: 0005-2787
    Electronic ISSN: 1879-3002
    Topics: Biology
    Published by Elsevier
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