Publication Date:
1996-04-05
Description:
The rotavirus nonstructural glycoprotein NSP4 is an intracellular receptor that mediates the acquisition of a transient membrane envelope as subviral particles bud into the endoplasmic reticulum. NSP4 also causes an increase in intracellular calcium in insect cells. Purified NSP4 or a peptide corresponding to NSP4 residues 114 to 135 induced diarrhea in young (6 to 10 days old) CD1 mice. This disease response was age-dependent, dose-dependent, and specific. Electrophysiologic data from intestinal mucosa showed that the NSP4 114-135 peptide potentiates chloride secretion by a calcium-dependent signaling pathway. Diarrhea is induced when NSP4, acting as a viral enterotoxin, triggers a signal transduction pathway.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ball, J M -- Tian, P -- Zeng, C Q -- Morris, A P -- Estes, M K -- DK 30144/DK/NIDDK NIH HHS/ -- New York, N.Y. -- Science. 1996 Apr 5;272(5258):101-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Molecular Virology, Baylor College of Medicine, Houston, TX 77030, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8600515" target="_blank"〉PubMed〈/a〉
Keywords:
*Aging
;
Amino Acid Sequence
;
Animals
;
Calcium/metabolism
;
Carbachol/pharmacology
;
Chlorides/metabolism
;
Colforsin/pharmacology
;
Diarrhea/*etiology/prevention & control/virology
;
Enterotoxins/*toxicity
;
Glycoproteins/immunology/*toxicity
;
Immune Sera/administration & dosage
;
Immunization
;
In Vitro Techniques
;
Intestinal Mucosa/drug effects/secretion
;
Mice
;
Molecular Sequence Data
;
Peptide Fragments/toxicity
;
Receptors, Virus
;
Rotavirus/*pathogenicity
;
Rotavirus Infections/prevention & control/*virology
;
Signal Transduction
;
Toxins, Biological
;
Viral Nonstructural Proteins/immunology/*toxicity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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