ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    EVOLUTION OF ORGANELLE GENOMES AND PROTEIN-SYNTHESIZING SYSTEMS (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 361 (1981), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1981.tb54355.x
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved (1989)
    Springer
    Journal of molecular evolution 29 (1989), S. 68-88 
    Details
    ISSN: 1432-1432
    Keywords: Ribsomal proteins ; Chloroplast ; Two-dimensional gel electrophoresis ; Immunological cross-reactivity ; Protein evolution ; Peptidyltransferase ; Anabaena 7120 ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Antibodies to individual chloroplast ribosomal (r-)proteins ofChlamydomonas reinhardtii synthesized in either the chloroplast or the cytoplasm were used to examine the relatedness ofChlamydomonas r-proteins to r-proteins from the spinach (Spinacia oleracea) chloroplast,Escherichia coli, and the cyanobacteriumAnabaena 7120. In addition,35S-labeled chloroplast r-proteins from large and small subunits ofC. reinhardtii were coelectrophoresed on 2-D gels with unlabeled r-proteins from similar subunits of spinach chloroplasts,E. coli, andAnabaena to compare their size and net charge. Comigrating protein pairs were not always immunologically related, whereas immunologically related r-protein pairs often did not comigrate but differed only slightly in charge and molecular weight. In constrast, when35S-labeled chloroplast r-proteins from large and small subunits of a closely related speciesC. smithii were coelectrophoresed with unlabeledC. reinhardtii chloroplast r-proteins, only one pair of proteins from each subunit showed a net displacement in mobility. Analysis of immunoblots of one-dimensional SDS and two-dimensional urea/SDS gels of large and small subunit r-proteins from these species revealed more antigenic conservation among the four species of large subunit r-proteins than small subunit r-proteins.Anabaena r-proteins showed the greatest immunological similarity toC. reinhardtii chloroplast r-proteins. In general, antisera made against chloroplast-synthesized r-proteins inC. reinhardtii showed much higher levels of cross-reactivity with r-proteins fromAnabaena, spinach, andE. coli than did antisera to cytoplasmically synthesized r-proteins. All spinach r-proteins that cross-reacted with antisera to chloroplast-synthesized r-proteins ofC. reinhardtii are known to be made in the chloroplast (Dorne et al. 1984b). FourE. coli r-proteins encoded by the S10 operon (L2, S3, L16, and L23) were found to be conserved immunologically among the four species. Two of the large subunit r-proteins, L2 and L16, are essential for peptidyltransferase activity. The third (L23) and two otherE. coli large subunit r-proteins (L5 and L27) that have immunological equivalents among the four species are functionally related to but not essential for peptidyltransferase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02106183
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A nuclear mutant of Chlamydomonas that exhibits increased sensitivity to UV irradiation, reduced recombination of nuclear genes, and altered transmission of chloroplast genes (1991)
    Springer
    Current genetics 19 (1991), S. 35-41 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Ultraviolet sensitive mutants ; Uniparental inheritance ; Chloroplast DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiotic progeny of Chlamydomonas reinhardtii normally receive chloroplast genomes only from the mt + parent. However, exceptional zygotes, which transmit the chloroplast genomes of both parents or, more rarely, only those of the mt - parent, arise at a low frequency. Mutations at the mt +-linked mat-3 locus were found previously to elevate the transmission of chloroplast genomes from the mt-parent, resulting in a much higher than normal frequency of exceptional zygotes. In this paper we demonstrate that an ultraviolet-sensitive nuclear mutation mapping at the uvsE1 locus, which is unlinked to mating type, also promotes chloroplast genome transmission from the mt - parent. This mutant, which was previously shown to reduce recombination of nuclear genes in meiosis, acts synergistically which the mat3-3 mutation to produce an extremely high frequency of exceptional zygotes. Through the use of restriction fragment length polymorphisms existing in the chloroplast genomes of C. reinhardtii and the interfertile strain C. smithii, we show that chloroplast DNA fragments from the mt - parent normally begin to disappear shortly after zygote formation. However, this process appears to be blocked totally in the absence of wild-type uvsE1 and mat-3 gene products. Our findings are consistent with the hypothesis that both gene products contribute to the mechanism responsible for uniparental inheritance of the chloroplast genome from the mt + parent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00362085
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Evidence for persistence of chloroplast markers in the heteroplasmic state in Chlamydomonas reinhardtii (1980)
    Springer
    Current genetics 2 (1980), S. 159-167 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Chloroplast ; Organelle genetics ; Streptomycin dependence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the green alga Chlamydomonas reinhardtii, reciprocal crosses between strains carrying non-allelic chloroplast mutations to streptomycin dependence (sd-u) produce streptomycin sensitive (sd-u +) recombinant progeny. Transfer of these sd-u +progeny to streptomycin-containing medium results in a much higher frequency of recovery of streptomycin dependent isolates than expected by mutation. Failure to recover the more commonly encountered class of streptomycin resistant mutants also suggests that mutation is not responsible for appearance of the new dependent isolates. Backcrosses of these new sd-u isolates to strains carrying the original sd-u mutations demonstrate their allelism with the sd-u mutation contributed by the mt +parent. Earlier work by Schimmer and Arnold (1969, 1970a-d) indicated that newly isolated sensitive revertants of the streptomycin dependent mutant sd-u-3-18 also yielded high frequencies of sd-u cells but these were never analyzed genetically. We have now obtained new sd-u. isolates from streptomycin sensitive revertants of sd-u-318 and shown them all to be allelic with the original sd-u3-18 mutation. Thus “hidden” sd-u alleles can coexist with sd-u +alleles in heteroplasmic cells. These heteroplasmic cells are streptomycin sensitive in phenotype and may arise in crosses or from new mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420628
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Mutations in a nuclear gene of Chlamydomonas cause the loss of two chloroplast ribosomal proteins, one synthesized in the chloroplast and the other in the cytoplasm (1984)
    Springer
    Current genetics 8 (1984), S. 369-378 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Non-photosynthetic mutations ; Ribosome biogenesis ; Chloroplast ribosomal proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The allelic nuclear mutations of Chlamydomonas reinhardtii, cr-6 and cr-7, result in the loss of two proteins from the large subunit of the chloroplast ribosome. One of these proteins, L-13, is synthesized in the chloroplast and the other, L29, is made in the cytoplasm. The loss of these two proteins is correlated with the inability of the large subunits of the chloroplast ribosomes to form monomers which incorporate labeled phenylalanine at normal rates in response to a polyuridylic acid template. Using antisera raised against L13 and L29, we found that protein L-13 was synthesized in appreciable amounts in pulse labeled cells of cr-6 and cr-7, but protein L-29 was not. We conclude that the inability to synthesize protein L29 is a primary defect in both cr-6 and cr-7 and that this protein is required for the stable assembly of protein L-13 into chloroplast ribosomes. The absence of one or both of these proteins from the large subunit of chloroplast ribosomes of the mutants interferes with the ability of the small and large subunits to associate properly into normal 70S monomers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419826
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Behavior of chloroplast genes during the early zygotic divisions of Chlamydomonas reinhardtii (1980)
    Springer
    Current genetics 1 (1980), S. 137-153 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Chloroplast genes ; Segregation ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplast mutations in the green alga Chlamydomonas reinhardtii exhibit a predominantly maternal pattern of inheritance and this pattern can be perturbed by UV irradiation of the maternal gametes prior to mating. In a series of crosses over a range of UV doses, the transmission, segregation, and recombination of mutations at three closely linked chloroplast loci have been examined by pedigree analysis of products arising from the first three post-zygotic divisions. Stocks used in these crosses were constructed to permit identification of the nuclear products of each of the two meiotic divisions and the first post-meiotic mitotic division. A bias toward maternal alleles at all three chloroplast loci was observed in all pedigrees and in zygote clones analyzed from the same crosses many generations after meiosis. This bias decreased with increasing UV dose and with each subsequent division. Segregation of chloroplast genes was rapid during the first three post-zygotic divisions. The type of segregation event from which a given heteroplasmic cell arose had a significant effect on its most likely segregation. pattern in the subsequent division. The results presented here have been discussed in terms of published models of chloroplast gene segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446960
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Specific elimination of mitochondrial DNA from Chlamydomonas by intercalating dyes (1987)
    Springer
    Current genetics 12 (1987), S. 41-47 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Mitochondrial DNA ; Acriflavin ; Ethidium bromide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Minute colony mutations in C. reinhardtii are induced with 100% efficiency by intercalating dyes such as acriflavin and ethidium bromide. These mutants form small colonies on petri plates because they undergo only 8–9 mitotic divisions before growth ceases. In liquid media without the dye the mutants show gross alterations in mitochondrial structure and function. Here we demonstrate that induction of minute mutations is accompanied by the specific loss of mitochondrial DNA. We also provide evidence that the transmission of the minute colony phenotype in crosses can be explained in terms of uniparental transmission of mitochondrial DNA by the mt − parent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420726
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Chloroplast genes encoding subunits of the H+-ATPase complex of Chlamydomonas reinhardtii are rearranged compared to higher plants: sequence of the atpE gene and location of the atpF and atpI genes (1987)
    Springer
    Plant molecular biology 8 (1987), S. 151-158 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ATP synthase ; physical mapping of chloroplast genes ; codon usage ; organization of subunit genes ; atpE DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3′ end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a position 92 kb away in the other single copy region. The uninterrupted open reading frame for the atpE gene is 423 bp, and the epsilon subunit exhibits 43% derived amino acid homology to that from spinach. Codon usage for the atpE gene follows the restricted pattern seen in other C. reinhardtii chloroplast genes. The genes for the CF0 subunits I (atpF) and IV (atpI) of the ATPase complex have also been mapped on the chloroplast genome of C. reinhardtii. The six chloroplast ATPase genes in C. reinhardtii are dispersed individually between the two single copy regions of the chloroplast genome, an organization strikingly different from the highly conserved arrangement in two operon-like units seen in chloroplast genomes of higher plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025326
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas (1984)
    Springer
    Plant molecular biology 3 (1984), S. 177-190 
    Details
    ISSN: 1573-5028
    Keywords: ATPase ; Chlamydomonas ; chloroplast ; complementation ; recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have carried out a molecular and genetic analysis of the chloroplast ATPase in Chlamydomonas reinhardtii. Recombination and complementation studies on 16 independently isolated chloroplast mutations affecting this complex demonstrated that they represent alleles in five distinct chloroplast genes. One of these five, the ac-u-c locus, has been positioned on the physical map of the chloroplast DNA by deletion mutations. The use of cloned spinach chloroplast ATPase genes in heterologous hybridizations to Chlamydomonas chloroplast DNA has allowed us to localize three or possibly four of the ATPase genes on the physical map. The beta and probably the epsilon subunit genes of Chlamydomonas CF1 lie within the same region of chloroplast DNA as the ac-u-c locus, while the alpha and proteolipid subunit genes appear to map adjacent to one another approximately 20 kbp away. Unlike the arrangement in higher plants, these two pairs of genes are separated from each other by an inverted repeat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016065
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Differential regulation of chloroplast gene expression in Chlamydomonas reinhardtii during photoacclimation: light stress transiently suppresses synthesis of the Rubisco LSU protein while enhancing synthesis of the PS II D1 protein (1997)
    Springer
    Plant molecular biology 33 (1997), S. 1001-1001 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast protein synthesis ; D1 ; LSU ; photoinhibition ; translational regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transfer of Chlamydomonas reinhardtii cells grown photoautotrophically in low light to higher light intensities has a dramatic transient effect on the differential expression of the two major chloroplast encoded photosynthetic proteins. Synthesis of the D1 protein of Photosystem II increases more than 10-fold during the first six hours in high light (HL), whereas synthesis of the large subunit (LSU) of Rubisco drops dramatically within 15 min and only gradually resumes at about 6 h. Synthesis of the chloroplast-encoded ATP synthaseβ subunit, the nuclear-encoded Rubisco small subunit and the nuclear-encoded β-tubulin is not noticeably affected. Up regulation of psbA mRNA translation accounts for a substantial fraction of the increased D1 synthesis, since accumulation of psbA mRNA increases 4.2- and 6.3-fold less than D1 synthesis at 6 and 18 h in HL. Down-regulation of LSU synthesis is not correlated with a reduction in the steady-state level of the rbcL transcript. Primer extension mapping of the 5' ends of the rbcL mRNAs reveals transcripts with start points located at -93 and -186 relative to the first translated ATG. Transfer of low light (LL)-grown cells to HL temporarily decreases the ratio of the -93 to -186 transcripts, but this ratio normalizes after 6 h in HL, coincident with the recovery in the synthesis of LSU. These several distinct effects of temporary light stress were correlated with a rapid, sustained increase in the reduction state of QA, a transient decline in photosynthetic efficiency, a less rapid drop in total chlorophyll content and a delay in cell division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005814800641
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...