ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
F4 progenies of a cross between durum wheat cultivar ‘Creso’ and an accession of Triticum turgidum ssp. dicoccoides with high protein content were analysed by two different electrophoretic procedures (A-PAGE and SDS-PAGE). Variation of storage proteins at the Glu-A1, Glu-B1, Glu-B3 and Gli-B1 loci was studied. Electrophoretic analyses have shown that parents contain different alleles at each of the four loci considered and that protein components of T. dicoccoides are uncommon among cultivated wheats. Recombination between the Glu-B3 and Gli-B1 loci was observed. To determine the effects of the allelic variants on gluten properties, F5 grains from every F4 line were analysed for protein content and SDS-sedimentation value. Gluten quality was strongly associated with the allelic type of proteins coded by the Glu-B3 locus and, to a lesser extent, to those coded by Glu-A1. Mean sedimentation value of progenies possessing the ‘Creso’Glu-B3 allele was significantly greater than that showing the T. dicoccoides allele. High molecular weight glutenin sub-units coded by the T. dicoccoides Glu-A1 locus were also associated with larger sedimentation values than null form of ‘Creso’. Results of SDS-test, obtained for recombinants between Glu-B3 and Gli-B1, confirmd that specific omega- and gamma-gliadins are only genetic markers of quality, whereas variation for LMW glutenin subunits coded at Glu-B3 is responsible for differences in gluten properties.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1439-0523.1991.tb00554.x
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