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  • 1
    Electronic Resource
    Electronic Resource
    Endosymbiotic bacteria GroEL buffers against deleterious mutations (2002)
    [s.l.] : Nature Publishing Group
    Nature 417 (2002), S. 398-398 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] GroEL, a heat-shock protein that acts as a molecular chaperone, is overproduced in endosymbiotic but not in free-living bacteria, presumably to assist in the folding of conformationally damaged proteins. Here we show that the overproduction of GroEL in Escherichia coli masks the effects of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/417398a
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of Colletotrichum species responsible for anthracnose of strawberry based on the internal transcribed spacers of the ribosomal region. (2000)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 189 (2000), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In recent years, different molecular techniques have led to an important progress in the characterisation of Colletotrichum species, but there are no available methods which permit the easy identification of Colletotrichum strains and their assignation to classical species. In the present work, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene, were used to identify a total of 80 strains of Colletotrichum, the majority of them isolated from strawberry. One of the most interesting results derived from this study was the easy and reliable distinction, using the endonuclease MvnI, between Colletotrichum fragariae and Colletotrichum gloeosporoides, both responsible of anthracnose on strawberry and phenotypically indistinguishable. Moreover, we propose the restriction fragments generated by the endonucleases MvnI, PvuII and ScrFI as a rapid method to differentiate species of the Colletotrichum genus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09213.x
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  • 3
    Electronic Resource
    Electronic Resource
    On the Evolution of Dopa decarboxylase (Ddc) and Drosophila Systematics (1999)
    Springer
    Journal of molecular evolution 48 (1999), S. 445-462 
    Details
    ISSN: 1432-1432
    Keywords: Key words:Dopa decarboxylase (Ddc) — Divergence —Drosophila—Scaptodrosophila—Chymomyza—Zaprionus—Scaptomyza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. We have sequenced most of the coding region of the gene Dopa decarboxylase (Ddc) in 24 fruitfly species. The Ddc gene is quite informative about Drosophila phylogeny. Several outstanding issues in Drosophila phylogeny are resolved by analysis of the Ddc sequences alone or in combination with three other genes, Sod, Adh, and Gpdh. The three species groups, melanogaster, obscura, and willistoni, are each monophyletic and all three combined form a monophyletic group, which corresponds to the subgenus Sophophora. The Sophophora subgenus is the sister group to all other Drosophila subgenera (including some named genera, previously considered outside the Drosophila genus, namely, Scaptomyza and Zaprionus, which are therefore downgraded to the category of subgenus). The Hawaiian Drosophila and Scaptomyza are a monophyletic group, which is the sister clade to the virilis and repleta groups of the subgenus Drosophila. The subgenus Drosophila appears to be paraphyletic, although this is not definitely resolved. The two genera Scaptodrosophila and Chymomyza are older than the genus Drosophila. The data favor the hypothesis that Chymomyza is older than Scaptodrosophila, although this issue is not definitely resolved. Molecular evolution is erratic. The rates of nucleotide substitution in 3rd codon position relative to positions 1 + 2 vary from one species lineage to another and from gene to gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00006489
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  • 4
    Electronic Resource
    Electronic Resource
    Phylogeny of the Drosophila obscura species group deduced from mitochondrial DNA sequences (1994)
    Springer
    Journal of molecular evolution 39 (1994), S. 478-488 
    Details
    ISSN: 1432-1432
    Keywords: Mitochondrial DNA ; Nucleotide sequences ; Drosophila ; Rapid phyletic radiation ; Molecular phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Approximately 2 kb corresponding to different regions of the mtDNA of 14 different species of the obscura group of Drosophila have been sequenced. In spite of the uncertainties arising in the phylogenetic reconstruction due to a restrictive selection toward a high mtDNA A+T content, all the phylogenetic analysis carried out clearly indicate that the obscura group is formed by, at least, four well-defined lineages that would have appeared as the consequence of a rapid phyletic radiation. Two of the lineages correspond to monophyletic subgroups (i.e., afftnis and pseudoobscura), whereas the obscura subgroup remains heterogeneous assemblage that could be reasonably subdivided into at least two complexes (i.e., subobscura and obscura).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173417
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  • 5
    Electronic Resource
    Electronic Resource
    Characterization of the length polymorphism in the A+T-rich region of the Drosophila obscura group species (1993)
    Springer
    Journal of molecular evolution 36 (1993), S. 214-223 
    Details
    ISSN: 1432-1432
    Keywords: Drosophila ; Mitochondrial DNA ; Length polymorphisms ; A+T-rich region ; Tandem duplicated sequences ; Nucleotide sequences ; Secondary structures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the twelve Drosophila obscura group species studied, belonging to the affinis, obscura, and pseudoobscura subgroups, the mitochondrial DNA length ranges from 15.8 to 17.2 kb. This length polymorphism is mainly due to insertions/deletions in the variable region of the A+T-rich region. In addition, one species (D. tristis) possess a tandem duplication of a 470-bp fragment that contains the replication origin. The same duplication has occurred at least twice in the Drosophila evolutionary history due to the fact that the repetition is analogous to repetitions found in the four species of the D. melanogaster complex. By comparing the nucleotide sequence of the conserved region in D. ambigua, D. obscura, D. yakuba, D. teissieri, and D. virilis, we show the presence of a secondary structure, likely implied in the replication origin, which could favor the generation of this kind of duplications. Finally, we propose that the high A and T content in the variable region of the A + T-rich region favors the formation of less-stable secondary structures, which could explain the generation of minor insertion/deletions found in this region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160476
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  • 6
    Electronic Resource
    Electronic Resource
    Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region (1998)
    Springer
    Archives of microbiology 169 (1998), S. 387-392 
    Details
    ISSN: 1432-072X
    Keywords: Key words Wine yeast identification ; rRNA gene ; RFLP-PCR ; Anamorph state ; Teleomorph state
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this study, we identified a total of 33 wine yeast species and strains using the restriction patterns generated from the region spanning the internal transcribed spacers (ITS 1 and 2) and the 5.8S rRNA gene. Polymerase chain reaction (PCR) products of this rDNA region showed a high length variation for the different species. The size of the PCR products and the restriction analyses with three restriction endonucleases (HinfI, CfoI, and HaeIII) yielded a specific restriction pattern for each species with the exception of the corresponding anamorph and teleomorph states, which presented identical patterns. This method was applied to analyze the diversity of wine yeast species during spontaneous wine fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050587
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  • 7
    Electronic Resource
    Electronic Resource
    RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces: a fast method for species identification and the differentiation of flor yeasts (2000)
    Springer
    Antonie van Leeuwenhoek 78 (2000), S. 87-97 
    Details
    ISSN: 1572-9699
    Keywords: flor yeasts ; identification ; ITS ; RFLP ; ribosomal DNA ; Saccharomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The PCR amplification and subsequent restriction analysis of the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene was applied to the identification of yeasts belonging to the genus Saccharomyces. This methodology has previously been used for the identification of some species of this genus, but in the present work, this application was extended to the identification of new accepted Saccharomyces species (S. kunashirensis, S. martiniae, S. rosinii, S. spencerorum, and S. transvaalensis), as well as to the differentiation of an interesting group of Saccharomyces cerevisiae strains, known as flor yeasts, which are responsible for ageing sherry wine. Among the species of the Saccharomyces sensu lato complex, the high diversity observed, either in the length of the amplified region (ranged between 700 and 875 bp) or in their restriction patterns allows the unequivocal identification of these species. With respect to the four sibling species of the Saccharomyces sensu stricto complex, only two of them, S. bayanus and S. pastorianus, cannot be differentiated according to their restriction patterns, which is in accordance with the hybrid origin (S. bayanus × S. cerevisiae) of S. pastorianus. The flor S. cerevisiae strains exhibited restriction patterns different from those typical of the species S. cerevisiae. These differences can easily be used to differentiate this interesting group of strains. We demonstrate that the specific patterns exhibited by flor yeasts are due to the presence of a 24-bp deletion located in the ITS1 region and that this could have originated as a consequence of a slipped-strand mispairing during replication or be due to an unequal crossing-over. A subsequent restriction analysis of this region from more than 150 flor strains indicated that this deletion is fixed in flor yeast populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002741800609
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  • 8
    Electronic Resource
    Electronic Resource
    Inter- and intraspecific chromosome pattern variation in the yeast genus Kluyveromyces (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 1341-1354 
    Details
    ISSN: 0749-503X
    Keywords: Kluyveromyces ; electrophoretic karyotyping ; contour-clamped homogeneous electric field electrophoresis ; chromosome variation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The analysis of the electrophoretic chromosome patterns of the species of the genus Kluyveromyces, reveals a high polymorphism in size, number and intensity of bands. Different sets of electrophoresis running conditions were used to establish species-specific patterns and also to detect intraspecific variation. According to their karyotypes, the species of this genus can be divided into two major groups. The first group includes the species K. africanus, K. bacillisporus, K. delphensis, K. lodderae, K. phaffi, K. polysporus and K. yarrowii, composing the so-called ‘Saccharomyces cerevisiae-like’ group, because their karyotypes resemble that of the species S. cerevisiae. The second group comprises the species K. aestuarii, K. blattae, K. dobzhanskii, K. lactis, K. marxianus, K. thermotolerans, K. waltii and K. wickerhamii, whose chromosomal patterns exhibit common characteristics very different to those of the species included in the ‘S. cerevisiae-like’ group. This division is concordant with the position of these species in previous phylogenetic reconstructions. Additionally, the intraspecific analysis of the chromosome patterns show a rich polymorphism in the heterogeneous species K. dobzhanskii, K. lactis, and K. marxianus, which is in concordance with the variability observed with other phenotypic or genetic markers. On the contrary, K. thermotolerans exhibits a homogeneous karyotype indicative of a very low level of chromosomal polymorphism, which is congruent with the reduced variability found in this species with other molecular markers. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
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  • 9
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    A Comparative Study of Different Methods of Yeast Strain Characterization (1992)
    Elsevier
    Details
    Publication Date: 1992-08-01
    Print ISSN: 0723-2020
    Electronic ISSN: 1618-0984
    Topics: Biology
    Published by Elsevier
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  • 10
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    Characterization of Wine Yeast Strains of the Saccharomyces Genus on the Basis of Molecular Markers: Relationships Between Genetic Distance and Geographic or Ecological Origin (1996)
    Elsevier
    Details
    Publication Date: 1996-03-01
    Print ISSN: 0723-2020
    Electronic ISSN: 1618-0984
    Topics: Biology
    Published by Elsevier
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