ISSN:
1432-1432
Keywords:
Maritime pine
;
2D electrophoresis
;
Genetic variability
;
Diversity
;
Organ-specific polypeptides
;
Mobility variants
;
Molecular weight
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Using two-dimensional polyacrylamide gel electrophoresis, the genetic variation of proteins was examined in three organs (needle, bud, and pollen) from 18 trees of maritime pine. Three types of variation were noted: presence/absence, staining intensity, and position variation of the spots. Of the 902 polypeptides scored in the three organs, 245 (27.2%) were polymorphic. Moreover, among these variable spots, 117 were found in a single organ, demonstrating an increased polymorphism of the organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific polypeptides). Finally, a positive correlation was found between variability level and subunit molecular weight for spots showing position variation but not for spots showing presence/absence or staining intensity variations. Possible explanations for this observation are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00170677
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