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  • 1
    Electronic Resource
    Electronic Resource
    The Ultrastructure of Tritrichomonas with Special Reference to the Blepharoplast Complex (1961)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 8 (1961), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Electron micrographs of sections through the blepharoplast complex reveal it to be composed of four kineto-somes. In many of the sections the kinetosomes contain dense particles approximately 180-200 A in diameter. The costa is a striated fiber which extends posteriorly almost the entire length of the body. Its slender proximal portion appears to be attached to the base of the kinetosome of the recurrent flagellum. The parabolas fiber is similarly constructed, but is more slender than the costa. It is attached to the base of the kinetosome of an interiorly directed flagellum and subsequently proceeds posteriorly to the level of the nucleus. The costa and parabasal fiber probably serve as anchoring or supportive structures. The size of the costa may reflect its role as a supporting structure for the most highly differentiated locomotors organelle, the undulating membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1961.tb01185.x
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  • 2
    Electronic Resource
    Electronic Resource
    The Fine Structure of the Heliozoan, Actinosphaerium nucleofilum (1960)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Actinosphaerium nucleofilum has been studied by light and electron microscopy. Thin sections of this organism reveal the relatively compact endoplasm to consist of numerous vacuoles and mitochondria. Scattered in the cytoplasm are dense particles, presumably ribonucleoprotein particles, endoplasmic reticulum of the rough variety and a tubular endoplasmic reticulum of the smooth variety. The many nucleido not appear to be randomly scattered in the cytoplasm but rather to be arranged in a circular pattern. Each is bounded by a double membrane envelope. A Golgi complex, consisting of isolated bodies with an ultrastructure similar to that described by others, is usually seen associated with each nucleus.The ectoplasm contains many vacuoles of varying diameter, each of which is limited by a thin membrane; vacuoles containing dense granular material, and mitochondria.The radially arranged axiopodia are extensions of the ectoplasm. The periphery is largely composed of vacuoles, some of which contain dense granules, and mitochondria. The axial rod is birefringent and consists of many fine filaments oriented parallel to the longitudinal axis of the axiopodium. The constituent filaments penetrate deep into the endoplasm where they end in the vicinity of nuclei. The fibrillar component of the axiopodia may represent contractile units.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1960.tb00729.x
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the Fine Structure of a Gregarine Parasitic in the Gut of the Grasshopper, melanoplus differentialis (1959)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 6 (1959), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Materials within the endoplasm of both the protomerite and deutomerite may be displaced and stratified by ultracentrifugation. Animals are not killed by this treatment, and in time a redistribution of stratified materials may occur. The electron microscope reveals numerous ultramicroscopic folds in the surface envelope which is composed of 3 membranes; the outer and middle cover the surface of the folds while the inner one continues as a smooth membrane over the ectoplasm. The contact surfaces of animals in syzygy do not show the small folds to be interlocked; instead, they are in fold-to-fold contact. Numerous relatively dense bodies which appear to be filamentous are sometimes observed immediately underneath or within the middle or inner membrane. Within the ectoplasm is an ultramicroscopic net-like fibrillar structure much denser than the surrounding cytoplasm. This system of fibrils is composed of anastomosing elements varying from ∼ 50 to 200 Å in diameter. Certain of the larger fibrils are directly attached to the surface envelope at positions between the folds. A second filamentous structure within the ectoplasm appears to be tubular. Elements of this system also appear to join and penetrate the surface envelope by means of pores.A large amount of gelatinous secretion forms at the surface of the sporont; as it glides forward a trail of mucous is left behind. The actual mechanism involved in the gliding movement was unobserved, but evidence is lacking to support the view that the gliding movement is engendered by posteriorly directed “jets” of mucus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1959.tb03940.x
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  • 4
    Electronic Resource
    Electronic Resource
    A Cytological Study of Chilomonas paramecium with Particular Reference to the So-called Trichocysts (1962)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Chilomonas paramecium has been studied by light and electron microscopy with special attention to the so-called trichocysts. The electron miscroscope reveals that the anatomy of these bodies is unlike that of the classical trichocysts of ciliates. Since these structures can be extruded from the body of the organism they have been called ejectisomes. The ejectisomes have a regular geometrical shape and a complex internal structure. They consist of two unequal components which are enclosed by a thin membrane. Morphological polarity is established by an anterior smaller unit and a posterior larger unit. When the ejectisomes are found in the peripheral cytoplasm or in a perivestibular position the smaller unit is always oriented toward the surface. A suggestion is made that the ejectisomes are derived from the vesicular component of the Golgi complex. An interpretation is offered concerning a mechanism by which ejectisomes may be extruded from the body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1962.tb02640.x
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  • 5
    Electronic Resource
    Electronic Resource
    The Electron Microscopy of Trichomonas muris (1955)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 2 (1955), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SUMMARY. Thin sections of Trichomonas muris, from the caecum of Mesocricetus auratus, were observed with the aid of the electron microscope in order to determine structural details of organelles of this species.The blepharoplast appears to be limited by a membrane. What seem to be basal granules occur in the blepharoplastic area for the axostyle and the lamellae of the undulating membrane and measure approximately 500 Å in diameter.In cross section the anterior flagella and posterior flagellum are composed of eleven fibrils, two centrally and nine peripherally located. Each fibril measures about 380 Å in diameter.The so-called “accessory filament” of the undulating membrane is devoid of any filamentous structure and appears to be composed of two differentiated mesh-work areas. The undulating membrane is composed of a series of lamellae ranging from 300 to 400 Å in thickness. This organelle is attached to the external body surface by fine fibers ranging from 167 to 300 Å in thickness.The costa consists of a series of disks embedded in a matrix. The costa is attached to the internal body surface by what appear to be extensions of the costal disks. These disks are approximately 370 Å in thickness and spaced 490 Å apart.The axostyle is limited by a double, corrugated membrane. The corrugations are about 150 Å in thickness and spaced 110 Å apart. The so-called chromatic ring is composed of a series of rods approximately 640 Å in thickness.The parabasal body consists of a series of filaments approximately 190 Å in thickness.Mitochondria, spheroidal in shape, are limited by a membrane about 180 Å in thickness. The internal structure consists of a varying number of projections which make this organelle appear in section as a series of compartments.Chromatic granules (paracostal, para-axostylar, endo-axostylar and those scattered in the cytoplasm) are irregular in shape and limited by a membrane approximately 200 Å in thickness. These granules typically display a vacuolated internal structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1955.tb02411.x
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  • 6
    Electronic Resource
    Electronic Resource
    The cytology of tritrichomonas as revealed by the electron microscopeThe contents of this paper were presented at a Round Table Discussion on The Ultrastructure of Protozoa held jointly by the Society of Protozoologists and the American Society of Zoologists at the American Institute for Biological Sciences meeting at Stanford University; Stanford, California, August 26-29, 1957. Supported by a grant (RG-4706 and 5479) from the National Institutes of Health, United States Public Health Service. (1959)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 104 (1959), S. 205-235 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051040203
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  • 7
    Electronic Resource
    Electronic Resource
    A study of oogenesis and early embryogenesis in the rabbit, Oryctolagus cuniculus, with special reference to the structural changes of mitochondriaThis investigation was supported by a grant GM 08776 from the National Institutes of Health, United States Public Health Service. The authors wish to thank Mrs. Gloria S. Lee and Mr. and Mrs. L. Musante for their technical assistance. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 130 (1970), S. 67-91 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The structural changes of mitochondria that occur during oogenesis and early embryogenesis in the rabbit have been examined with the electron microscope. Mitochondria of oogonia are both elongate and oval and contain a variable number of cristae which may or may not traverse the longitudinal axis of the organelle. When oogonia differentiate into oocytes, mitochondria become spheroidal and their cristae are sparse when compared with those found in the ellipsoidal organelles of concomitantly maturing follicle cells. As differentiation proceeds, the cristae of the mitochondria display varied configurations. For example, many display an arch-like arrangement in several regions of the organelle whereas others contain a pair of concentric membranes closely associated with limiting membrane of the mitochondrion.Mitochondria of stages from the fertilized egg to the morula possess the same internal structure as those of young oocytes. As the morula differentiates into a blastocyst there is a gradual increase in the size of the mitochondria and an increase in the number of cristae. We believe that the number and modifications of cristae indicate stages of mitochondriogenesis and the level of enzymatic activity in which this organelle is engaged during oogenesis and early embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051300108
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  • 8
    Electronic Resource
    Electronic Resource
    Fine structure of “intracellular ductules” in certain glands of the carabid beetleThis investigation was aided by grants RG-4706 and 5479 from the National Institutes of Health, United States Public Health Service, and grant G-9879 from the National Science Foundation. (1961)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 109 (1961), S. 159-171 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051090205
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  • 9
    Electronic Resource
    Electronic Resource
    Cytochemical and fine structural analysis of oogenesis in the gastropod, Ilyanassa obsoletaThis investigation was supported by a grant (GM 08776) to one of us (EA) from the National Institutes of Health, United States Public Health Service. (1969)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 129 (1969), S. 211-247 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An analysis of differentiating oocytes of the gastropod, Ilyanassa obsoleta, has been made by techniques of light and electron microscopy. Early previtellogenic oocytes are limited by a smooth surfaced oolemma and are associated with each other by maculae adhaerentes. Previtellogenic oocytes are also distinguished by a large nucleus containing randomly dispersed aggregates of chromatin. Within the ooplasm are Golgi complexes, mitochondria and a few cisternae of the rough endoplasmic reticulum. When vitellogenesis begins, the oolemma becomes morphologically specialized by the formation of microvilli. One also notices an increase in the number of organelles and inclusions such as lipid droplets. During vitellogenesis there is a dilation of the saccules of the Golgi complexes and cisternae of the endoplasmic reticulum. Associated with the Golgi complexes are small protein-carbohydrate yolk precursors encompassed by a membrane. These increase in size by fusing with each other. The “mature” yolk body is a membrane-bounded structure with a central striated core and a granular periphery. At maturity a major portion of the ooplasmic constituents such as as mitochondria and lipid droplets occupy the animal region while the bulk of the population of yolk bodies are situated in the vegetal hemisphere.The follicle cells incompletely encompass the developing oocyte. In addition to the regularly occurring organelles, follicle cells are characterized by the presence of large quantities of rough endoplasmic reticulum and Golgi complexes whose saccules are filled with a dense substance. Associated with the Golgi saccules are secretory droplets of varied size.Amongst the differentiating oocytes and follicle cells are Leydig cells. These cells are characterized by a large vacuole containing glycogen. A possible function for the follicle and Leydig cells is discussed.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051290206
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  • 10
    Electronic Resource
    Electronic Resource
    A correlative microscopical analysis of differentiating ovarian follicles of mammalsThis investigation was supported by Grants (HD 06822; HD 06645) from the National Institutes of Health and the United States Public Health Service. (1978)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 156 (1978), S. 339-366 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The mammalian ovary has been studied by optical microscopy and by scanning and transmission electron microscopy with the purpose of presenting an integrated view of the differentiating mammalian follicle. During follicular development, changes in the granulosa cells are particularly noteworthy and include dramatic modifications in cell shape coincident with antrum formation. The cytoplasmic processes of those granulosa cells immediately surrounding the oocyte, as well as the more peripheral granulosa cells comprising a second and third layer, traverse the zona pellucida, infrequently interdigitate with the microvilli of the egg, and make both desmosomal and gap junction contacts with the oocyte. The zona pellucida is thus distinguished by numerous fenestrations of varying diameters. The membrana limitans (basal lamina) is a bipartite structure composed of (a) a homogeneous stratum upon which the peripheral layer of granulosa cells rests, and (b) an outer region of collagen-like fibers. The specific advantages and limitations of the different methodologies utilized to study folliculo-genesis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051560303
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