Publication Date:
2021-05-19
Description:
The study was done in Nutrition and Live Food Station was located in Bandar Anzali Ghaziyan. Juveniles
weighted average 0.5, 1, 2.5, 5, 10, 15 and 20 g were randomly selected in three water conditions with a salinity
of 11 ppt (Caspian sea water), water 7 ppt and freshwater (with three replicates per group) were included.
At intervals of 0, 3, 6, 12, 24, 72, 168, 240 hours, blood samples were heparinized capillary tubes by caudal
juveniles and ion concentrations Mg, Ca,Cl using the spectrophotometer and sodium and potassium ions with
Flaym photometry (flame photometric), the osmotic pressure of blood plasma by osmometer and cortisol levels
were measured by RIA method.
To study the microstructure of gill and kidney tissue for each treatment , tissue samples by classical histological
methods and stained with hematoxylin - eosin slides were prepared. The frequency and location of the enzyme
Na +, K + - ATPase and chloride cells with immunohistochemical localization was performed. Studies
micrometric gill chloride cells and renal glomerular networks by software Image tool (version 2.0) was
performed. Measurement of enzyme Na +, K +-ATPase, by Zaugg (1982) method was conducted. Data analyzed
by one-way ANOVA (Oneway ANOVA) with Tukey's test was performed. Overall, the results of measuring
ions and osmotic pressure on the tenth day of treatment, the osmotic potential juveniles 2.5, 5, 10, 20 gr in
Caspian sea water and all groups except the 0.5 in water of 7 ppt confirmed. But in case of unfavorable
conditions for the release in estuaries river and river, fish with weight 1 to 3 release directly to beach (where the
salinity is 7 grams per liter) and fishes with weight from 10 to 20 gr to sea. Although suitable river conditions
necessary condition for release of juveniles in riverine areas to adaptation juveniles occur gradually.
Description:
Iranian Fisheries Science Research Institute
Description:
Published
Keywords:
Rutilus frisii kutum
;
Osmolarity
;
Suitable size
;
Release
Repository Name:
AquaDocs
Type:
Report
,
Refereed
Format:
54pp.
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