Publication Date:
2012-11-10
Description:
Fluorescent proteins (FPs) are widely used as optical sensors, whereas other light-absorbing domains have been used for optical control of protein localization or activity. Here, we describe light-dependent dissociation and association in a mutant of the photochromic FP Dronpa, and we used it to control protein activities with light. We created a fluorescent light-inducible protein design in which Dronpa domains are fused to both termini of an enzyme domain. In the dark, the Dronpa domains associate and cage the protein, but light induces Dronpa dissociation and activates the protein. This method enabled optical control over guanine nucleotide exchange factor and protease domains without extensive screening. Our findings extend the applications of FPs from exclusively sensing functions to also encompass optogenetic control.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702057/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702057/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhou, Xin X -- Chung, Hokyung K -- Lam, Amy J -- Lin, Michael Z -- R01 NS076860/NS/NINDS NIH HHS/ -- R01NS076860/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 2012 Nov 9;338(6108):810-4. doi: 10.1126/science.1226854.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23139335" target="_blank"〉PubMed〈/a〉
Keywords:
Adaptor Proteins, Vesicular Transport/chemistry/genetics/metabolism
;
Animals
;
Cell Membrane/metabolism
;
Darkness
;
Fluorescence
;
HeLa Cells
;
Humans
;
*Light
;
Luminescent Proteins/*chemistry/genetics/metabolism
;
Mice
;
Models, Molecular
;
NIH 3T3 Cells
;
Native Polyacrylamide Gel Electrophoresis
;
Optogenetics
;
Protein Conformation
;
Protein Engineering
;
Protein Multimerization
;
*Protein Structure, Tertiary
;
Pseudopodia/metabolism/ultrastructure
;
Recombinant Fusion Proteins/*chemistry/genetics/metabolism
;
Serine Endopeptidases/chemistry/genetics/metabolism
;
Viral Nonstructural Proteins/chemistry/genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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