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  • 1
    Electronic Resource
    Electronic Resource
    DNA sequences of the two homoeologous SLR1 genes of Brassica napus cv Westar (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 254-255 
    Details
    ISSN: 1432-2145
    Keywords: Brassica napus ; SLR1 genes ; Pollination ; Stigma-specific
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228946
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  • 2
    Electronic Resource
    Electronic Resource
    Genomic sequence of an SRK-like gene linked to the S-locus of a self-incompatible Brassica oleracea line (1995)
    Springer
    Sexual plant reproduction 8 (1995), S. 247-253 
    Details
    ISSN: 1432-2145
    Keywords: Brassica ; Self-incompatibility ; S-receptor kinase gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone of an S-locus glycoprotein gene (SLG) from Brassica oleracea specific to the S 29 self-incompatibility allele was used to detect homologous gene sequences in the S 63 haplotype. Two genomic fragments hybridised by this probe appeared to comprise an SLG and S-receptor kinase (SRK) gene pair, as previously described in B.oleracea. The complete DNA sequence of the putative receptor kinase gene was determined and found to predict a polypeptide structurally homologous to SRK proteins. Combined RFLP, stigma glycoprotein and pollination analysis of a segregating F2 family of plants indicated that the gene pair is linked to the S 63 incompatibility allele. Transcripts originating from the SRK-like gene were identified in stigma mRNA by DNA sequencing of amplified RT-PCR products but homologous transcripts were also found in leaf. An associated stigma-specific SLG transcript was not detected, suggesting that this SRK-like gene may have a function and tissue-specificity unrelated to SI and that the true SRK 63 allele may have a diverged DNA sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228945
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  • 3
    Electronic Resource
    Electronic Resource
    Unexpectedly slow homogenisation within a repetitive DNA family shared between two subspecies of tsetse fly (1984)
    Springer
    Journal of molecular evolution 20 (1984), S. 322-329 
    Details
    ISSN: 1432-1432
    Keywords: Repetitive DNA families ; Homogenisation ; Sequence variation ; Tsetse fly species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Repetitive DNA families in sexual species are subject to a variety of turnover mechanisms capable of homogenising newly arising mutations. Very high levels of homogeneity in DNA families in some species ofDrosophila indicate that the rate of turnover is fast relative to that of mutation. To gauge the generality of such phenomena, we cloned and sequenced individual members of homologous repetitive DNA families from two subspecies of tsetse fly,Glossina morsitans centralis andG. morsitans morsitans. Unexpectedly high levels of variation were found within each subspecies, averaging 24% and 31%, respectively. Contiguous repeats and repeats cloned at random were comparably divergent. Nevertheless, it was possible to identify three instances of apparent homogenisation, each being, remarkably, of an insertion/deletion nature. We conclude that the rate of turnover in the tsetse families is comparable to that of most mutations, and discuss the possible parameters affecting flux in these families.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02104738
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  • 4
    Electronic Resource
    Electronic Resource
    Cryptic simplicity in DNA is a major source of genetic variation (1986)
    [s.l.] : Nature Publishing Group
    Nature 322 (1986), S. 652-656 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To assess the frequencies and types of simple sequence motifs, we made a systematic survey of published sequences17 using two criteria. The first was to scan for direct sequence homologies to 15-nucleotide-long probes, each consisting of an array of one of all possible mono-, di- and trinucleotide ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/322652a0
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence complexity of the S receptor kinase gene family in Brassica (1993)
    Springer
    Molecular genetics and genomics 241 (1993), S. 440-446 
    Details
    ISSN: 1617-4623
    Keywords: Brassica ; Self-incompatibility ; S-receptor kinase ; Gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genomic library from an S 29/S 29 self-incompatible genotype of Brassica oleracea was screened with a probe carrying part of the catalytic domain of a Brassica S-receptor kinase (SRK)-like gene. Six positive phage clones with varying hybridisation intensities (K1 to K6) were purified and characterised. A 650–700 by region corresponding to the probe was excised from each clone and sequenced. DNA and predicted protein sequence comparisons based on a multiple alignment identified K5 as a pseudogene, whereas the others could encode functional proteins. K3 was found to have lost an intron from its genomic sequence. The six genes display different degrees of sequence similarity and form two distinct clusters in a dendrogram. The 98% similarity between K4 and K6, which extends across intron sequences, suggests that these might be very recently diverged alleles or daughters of a duplication. In addition, K2 showed a comparably high similarity to the probe. Clones K1, K3 and K5 cross-hybridised with an SLG 29 cDNA probe, indicating the presence of upstream receptor domains homologous to the Brassica SLG gene. This suggests that the previously reported S sequence complexity may be ascribed to a large receptor kinase gene family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00284698
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  • 6
    Electronic Resource
    Electronic Resource
    Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes (1998)
    Springer
    Plant molecular biology 38 (1998), S. 1081-1087 
    Details
    ISSN: 1573-5028
    Keywords: fluorescence in situ hybridization ; Brassica napus ; S-locus ; rDNAs ; image analysis ; quantitative chromosome map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seventy years after Karpechenko [15] first reported the accurate chromosome number of oilseed rape (Brassica napus L., 2n=38), we have developed a quantitative chromosome map of rape using computer imaging technology. The capacity to identify individual rape chromosomes will facilitate a wide range of genetic studies. Here we demonstrate the use of imaging methods in combination with fluorescence in situ hybridization to localize, on identified chromosomes, the single copy S-locus glycoprotein and S-locus-related genes involved in the self-incompatibility system of Brassica. These techniques have a broader application in plant genome research involving the mapping of single-copy genes and markers, irrespective of the plant species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006036100987
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  • 7
    Electronic Resource
    Electronic Resource
    Molecular analysis of the alcohol dehydrogenase gene family of barley (1988)
    Springer
    Plant molecular biology 11 (1988), S. 147-160 
    Details
    ISSN: 1573-5028
    Keywords: alcohol dehydrogenase ; barley ; DNA sequences ; gene duplication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract One partial and two complete genomic clones of the three loci specifying alcohol dehydrogenase (ADH) in barley were isolated by screening libraries with a maize Adh1 cDNA probe. Each gene is characterised by an intron arrangement similar to that of both maize Adh1 and Adh2, although two genes show an exon fusion. A comparison with the maize coding sequences unambiguously assorts the barley loci into an Adh1-like gene and two Adh2-like genes, indicating that an ancient gene duplication underlies the widespread occurrence of two Adh loci in higher plants. In the barley lineage there has been a further duplication-transposition of a progenitor “Adh2” locus to give rise to the extant three-gene system, with gene copies of different ancestry being closely linked. An Adh1 null-allele, Adh1-M9, has been cloned; the available sequence includes an intron with a missing acceptor splice signal. Two independent clones of one of the barley Adh2-like genes have an 18 bp in-frame deletion towards the 3′ end of the coding sequence. The barley Adh2-like genes are extensively diverged in their 5′ sequences apart from a conserved 15 bp motif in the mRNA leader region and sequences at the start of transcription. A sequence related to the hexanucleotide core of a regulatory element found in maize Adh1 and in other anaerobically induced plant genes is present in the 5′ region of barley Adh2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015667
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  • 8
    Electronic Resource
    Electronic Resource
    A homozygous S genotype of Brassica oleracea expresses two S-like genes (1989)
    Springer
    Molecular genetics and genomics 218 (1989), S. 112-117 
    Details
    ISSN: 1617-4623
    Keywords: Self-incompatibility ; Brassica ; S locus-specific glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sporophytic self-incompatibility system of Brassica species is controlled by a single locus, S. Recognition of self between pollen and stigma is probably mediated by S locus-specific glycoproteins (SLSGs). We describe the isolation, from an S 29 homozygote of Brassica oleracea, of two different cDNA clones for transcripts which are equally abundant in stigmas competent for self-incompatibility and each of which is homologous to previously reported SLSG sequences. Extensive DNA sequence divergene between the two clones precludes their cross-hybridisation and each acts as a gene-specific probe. All S genotypes appear to have a single copy of each gene but there are significantly different levels of polymorphism associated with each. The clear structural homology between the two indicates a gene duplication involving the S locus and, perhaps, related to the evolution of self-incompatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330573
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  • 9
    Electronic Resource
    Electronic Resource
    Sequence and expression of endogenous S-locus glycoprotein genes in self-compatible Brassica napus (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 209-216 
    Details
    ISSN: 1617-4623
    Keywords: Brassica ; Self-incompatibility ; Self-compatibility ; S-locus glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Brassica napus is an amphidiploid plant which is self-compatible even though it is derived from hybridisation of the self-incompatible species B. oleracea and B. campestris. Experiments were undertaken to establish if S-locus glycoprotein (SLG) genes exist in B. napus and whether these are expressed as in self-incompatible Brassica species. Two different stigma-specific cDNA sequences homologous to SLG genes were obtained from the B. napus cultivar Westar. One of these sequences, SLG WS1, displayed highest homology to class I SLG alleles, whereas the other, SLG WS2, showed greatest homology to class II SLG genes. Both were expressed at high levels in Westar stigmas following a developmental pattern typical of SLG genes in the self-incompatible diploids. We infer that they represent the endogenous SLG genes at the two homoeologous S-loci. The occurrence of normally expressed SLG genes and its relevance to the self-compatible phenotype of B. napus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391015
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  • 10
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    The genome of the mesopolyploid crop species Brassica rapa (2011)
    Springer Nature
    Details
    Publication Date: 2011-08-28
    Print ISSN: 1061-4036
    Electronic ISSN: 1546-1718
    Topics: Biology , Medicine
    Published by Springer Nature
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