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  • 1
    Electronic Resource
    Electronic Resource
    Replication of ϕA and ϕX174 in Escherichia coli mutants thermosensitive in DNA synthesis (1975)
    Springer
    Molecular genetics and genomics 139 (1975), S. 285-291 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thermosensitive mutants of E. coli defective in DNA replication were tested for their capacity to support multiplication of ϕA and ϕX174. At the restrictive temperature, the viral growth was markedly affected in dnaH, dnaZ or ligts7 mutants. Even when these strains were transfected with RF1 molecules, the virus yield was still very low. The dnaI function was, however, dispensable for replication of ϕA and ϕX174. In addition, these viruses could multiply in dnaP or polA ts mutants at the high temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267968
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of the dnaN mutation on the growth of small DNA phages (1981)
    Springer
    Molecular genetics and genomics 183 (1981), S. 130-133 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of the dnaN mutation on the growth of single-stranded DNA phages was studied by burst experiments. In HC138 dnaN cells exposed to 42.5° C at 5 min before infection, growth of spherical (microvirid or isometric) phages such as α3, πKh-1 and πX174 was partially reduced at the nonpermissive temperature. When infection was performed at 30 min after temperature shift-up, viral replication was completely inhibited at 42.5° C in the dnaN strain but not in a dna + revertant. At 41° C, multiplication of filamentous (inovirid) phages M13 and fd was restricted specifically in HC138 F+ dnaN bacteria. When dnaN cells lysogenic for λi21 were grown at 42.5° C for 60 min and then shifted down to 33° C, a burst of λi21 occurred with concomitant cellular lysis, manifesting induction of the prophage development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270150
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  • 3
    Electronic Resource
    Electronic Resource
    Sensitivity of Escherichia coli to viral nucleic acid (1973)
    Springer
    Molecular genetics and genomics 122 (1973), S. 15-22 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seven groups of dna mutants were tested for the capacity to support the growth of ϕA and ϕX174, using a calcium-dependent transfection system. At the restrictive temperature, two groups of mutants, dnaA and dnaF, allowed the viral multiplication. Group B, C, D, E and G mutants were nonpermissive at 43°C to SS1 DNA as well as to double-stranded RF molecule. Evidence showing the dispensability for the viral growth of DNA polymerase I and recombination function was also presented. Double mutant deficient in DNA polymerase I and II supported the growth of ϕA sufficiently.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00337970
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  • 4
    Electronic Resource
    Electronic Resource
    Host factor requirements and some properties of ΦXtB (1976)
    Springer
    Molecular genetics and genomics 148 (1976), S. 139-142 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Replication of ΦXtB, a capsid mutant of bacteriophage ΦX174, depends on the host functions directed by the E. coli genes dnaE, dnaF, dnaG, dnaZ, lig and rep. The cellular products of dnaA, dnaB, dnaC(D), dnaI, dnaP, polA, polB and xth genes are, however, dispensable for the viral growth. In these host factor requirements, ΦXtB resembles phages ΦK and St-1, rather than ΦX174. Host ranges of ΦXtB, St-1 and ΦK overlap considerably, and growth temperature of the three phages is somewhat higher than that of ΦX174. Furthermore, ΦXtB is, like ΦK, inactivated by antiserum against St-1. ΦXtB may thus fill an evolutionary gap between the ΦX174 group and the St-1 group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268377
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and some properties of bacteriophage α3 gene J mutant (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 541-543 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To elucidate the in vivo function of the J gene of microvirid (isometric) phages, we isolated several strains carrying a double mutation in J and H genes from phage α3 and then constructed single mutants each having an amber codon in the J gene. The J mutants could not multiply in suppressor-less hosts and were deficient in single-stranded progeny DNA synthesis. Nucleotide sequences of the wild-type and mutant α3 J genes were analyzed to determine the mutation sites. The amino acid sequence of the J gene was also deduced from the nucleotide sequence and compared with those of ϕX174 and G4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00341460
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  • 6
    Electronic Resource
    Electronic Resource
    Host genes involved in the replication of single-stranded DNA phage ϕK (1976)
    Springer
    Molecular genetics and genomics 148 (1976), S. 25-29 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using various replication mutants of E. coli, the host genes that participate in the replication of some K12-specific single-stranded DNA phages have been determined. Functional products of dnaE,-F,-G and -Z genes are required for the multiplication of ϕK, whereas dnaA,-B,-C(D), H,-I and -P are dispensable for viral replication. In contrast with polB, recA, B, C, or xth functions, host rep activity is essential for ϕK. At the restrictive temperature, the yield of ϕK was markedly reduced in the ligts7 mutant and partially decreased in a polA ts strain. The phage ϕK is thus less dependent on the host cells than ϕX174 and ϕA which require additionally the dnaB,-C(D) and -H functions. Replication of phage St-1 depends on dnaG and -Z gene products, but not on dnaP function. Although not much affected in polA ts host, growth of St-1 was significantly diminished in dnaF or ligts7 mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268542
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  • 7
    Electronic Resource
    Electronic Resource
    Growth of single-stranded DNA phages in replication mutants of Escherichia coli (1978)
    Springer
    Molecular genetics and genomics 162 (1978), S. 151-155 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Host capacity for growth of single-stranded DNA phages was investigated with several replication mutants of E. coli. In dnaL708, dnaM709 and dnaS707 mutants, multiplication of ϕK was not restricted even at 42°C. In dnaM710 cells, however, growth of ϕK was severely affected at 42°C but not at 33°C. Upon infection of ϕK, parental replicative form was synthesized at the restrictive temperature, whereas subsequent step (replication of progeny replicative form) was blocked in the dnaZ strain. Growth of ϕX174 and α3, as tested by transfection, was also thermosensitive in the dnaM710 mutant but not in the dnaL708, dnaM709 and dnaS707 strains. In contrast with λ, microvirid phages could grow in E. coli cells bearing the groPC259, groPC756 or seg-2 mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267871
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  • 8
    Electronic Resource
    Electronic Resource
    Novel replication mutant of microvirid phage α3 deleted in the complementary strand origin (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 240-244 
    Details
    ISSN: 1617-4623
    Keywords: Bacteriophage α3 ; ori ; Deletion mutant ; dnaB-C requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The bacteriophage α3 origin of complementary strand DNA synthesis (—ori) contains two potential secondary loop structures (I and II), which have been implicated as direct recognition sites for host Escherichia coli DnaG protein. To elucidate to what extent such structures are essential, we introduced a nucleotide deletion within the —ori region, by nuclease digestion of α3 replicative form DNA. A mutant, delB, thus constructed had a 121 nucleotide deletion within the —ori region and was completely lacking in the two putative hairpin loops, I and II. The delB mutant formed smaller plaques on the host E. coli C and had a longer latent period, but the mean burst size at 37° C was almost the same (400 phages) as that of the wild type. In contrast to the parental phage, growth of the mutant depends on host dnaB and dnaC functions. These results indicate that the prototype secondary structures in the α3 origin of complementary strand synthesis are dispensable for delB and that the α3 mutant has an additional replication origin whose function is dependent on DnaB and DnaC proteins, rather than on DnaG protein alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260488
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  • 9
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    Biochemical Studies on Streptolysin S Formation* (1964)
    Oxford University Press
    Details
    Publication Date: 1964-12-01
    Print ISSN: 0021-924X
    Electronic ISSN: 1756-2651
    Topics: Biology , Chemistry and Pharmacology
    Published by Oxford University Press on behalf of Japanese Biochemical Society.
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  • 10
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    Nucleoside Catabolism in Streptococcus hemolyticus (1974)
    Oxford University Press
    Details
    Publication Date: 1974-06-01
    Print ISSN: 0021-924X
    Electronic ISSN: 1756-2651
    Topics: Biology , Chemistry and Pharmacology
    Published by Oxford University Press on behalf of Japanese Biochemical Society.
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