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  • 1
    Electronic Resource
    Electronic Resource
    The uvrD gene of E. coli encodes a DNA-dependent ATPase (1982)
    [s.l.] : Nature Publishing Group
    Nature 298 (1982), S. 98-100 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A hybrid phage, \uvrD+, which is able to complement uvrD mutations, was constructed using Agt-Ac as cloning vector11. In addition to the uvrD gene, \uvrD+ carries the cor A gene that controls transport of Mg2+, Mn2+ and Co2+ through the cell membrane12'13. A hybrid phage, AcorA+, which carries the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/298098a0
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  • 2
    Electronic Resource
    Electronic Resource
    Protein function Chaperonin turned insect toxin (2001)
    [s.l.] : Nature Publishing Group
    Nature 411 (2001), S. 44-44 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Antlions are larvae of the Myrmeleontidae family that live on other insects by sucking out the body fluid from their prey, after first paralysing it with a toxin produced by salivary bacteria. Here we show that the paralysing toxin produced by bacterial endosymbionts in the saliva of Myrmeleon ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35075148
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  • 3
    Electronic Resource
    Electronic Resource
    Positive and negative cis-regulatory regions in the soybean glycinin promoter identified by quantitative transient gene expression (1995)
    Springer
    Plant cell reports 14 (1995), S. 539-544 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 5′ and 3′ flanking regions of the soybean glycinin gene, Gy1, responsible for expression in seeds, were analyzed by quantitative transient expression assay. The construct containing the β-glucuronidase (uidA) reporter gene under the control of the 1.12 kb Gy1 promoter and 0.74 kb Gy1 terminator was introduced into immature soybean seeds and leaves by particle bombardment. To normalize the variability of introduction efficiency, a second reporter gene, firefly luciferase, was cobombarded as an internal standard, and relative activities (GUS/luciferase) were measured. There was a seed-specific β-glucuronidase (GUS) expression, as observed by X-Gluc staining. Compared with the nopaline synthase gene (nos) terminator, the Gy1 terminator enhanced the level of expression in immature seeds, indicating that the terminator region of the glycinin gene is involved in the activation of the gene expression in these seeds. To identify cis-regulatory elements in the glycinin gene upstream sequence, deleted derivatives of the promoter were fused to the luciferase reporter gene. The expression could be measured with a higher accuracy, and constructs were introduced with the internal reporter uidA gene into immature seeds. The results suggest the presence of a positive regulatory element in the −620 to −−380 region of the Gy1 promoter. A deletion which eliminates the legumin box with its RY element led to increased relative activity, suggesting that this box is negatively regulating expression of the seed storage protein gene. Analysis of mutant promoters also suggest that the RY element involves negative regulation in seeds. This quantitative transient expression assay using particle bombardment provides a reliable system for the study of seed-specific gene expression in soybeans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231934
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  • 4
    Electronic Resource
    Electronic Resource
    A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity (1992)
    Springer
    Current genetics 21 (1992), S. 399-404 
    Details
    ISSN: 1432-0983
    Keywords: Neurospora ; Beta-tubulin ; Benzimidazoles ; N-phenylcarbamates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two MBC-resistant mutants of Neurospora crassa, F914 and F939, were sensitive to diethofencarb at a concentration of 0.1 μg/ml, while the wild-type strain and other MBC-resistant mutants showed resistance to diethofencarb at a concentration of 100 μg/ml. Genetic analysis suggested that the mutations in these two strains were closely linked to the Bml locus which codes for beta-tubulin. When the wild-type strain was transformed by the cloned beta-tubulin gene of the F914 strain, the transformants showed both MBC resistance and diethofencarb sensitivity. On the other hand, the diethofencarb sensitivity of the F914 strain was cancelled by transformation with the wild-type beta-tubulin gene. DNA sequencing of F914 beta-tubulin revealed that glycine was substituted for glutamic acid at position 198 in the F914 strain. Therefore, a single base change in the betatubulin gene was proved to confer both MBC resistance and diethofencarb sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351701
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency (1981)
    Springer
    Molecular genetics and genomics 184 (1981), S. 191-199 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The uvrD gene of Escherichia coli that control UV sensitivity and spontaneous mutation frequency has been cloned with phage λ as vector. The increased sensitivity to ultraviolet light (UV) of uvrD3, uvrE502, recL152, and pdeB41 mutants, high mutability of uvrD3 and pdeB41 mutants, and conditional lethality of strain TS41 that carried pdeB41, polA1, and sup126 mutations were all suppressed by lysogenization of the mutant cells with λuvrD +. These results were consistent with the idea that the uvrD, uvrE, recL, and pdeB mutations are alleles of the uvrD gene. In addition to the uvD gene, λuvrD + carried the corA gene that controls transport of Mg++, Mn++, and Co++ through the cell membrane. Hybrid plasmids carrying both uvrD and corA genes were also constructed by using pKY2289 as a cloning vehicle. Orientational isomers that carried the same 12.0 kb fragment in the opposite direction were equally efficient in complementing the UvrD- as well as CorA- defects of the transformed host cells, suggesting that the DNA insert contains all the genetic signals needed to express the two gene products. Insertion of the γδ sequence into recombinant plasmids was performed to generate appropriate restriction endonuclease target sites in the cloned DNA fragments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272904
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  • 6
    Electronic Resource
    Electronic Resource
    DNA polymorphisms in Oryza sativa L. and Lactuca sativa L. amplified by arbitrary primed PCR (1994)
    Springer
    Euphytica 78 (1994), S. 143-148 
    Details
    ISSN: 1573-5060
    Keywords: DNA fingerprint ; Lactuca sativa ; Oryza sativa ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Thirty-five rice (Oryza sativa L.) varieties, including 18 japonica, 5 javanica and 12 indica subspecies and 12 lettuce (Lactuca sativa L.) varieties were identified taxonomically, using PCR with originally designed 21 RAPD (Random Amplified Polymorphic DNA) primers and 8 sequence-specific primers, used for amplifying four specific DNA fragments. Use of these primers revealed polymorphisms among varieties in rice and lettuce and facilitates DNA fingerprinting. Dendrograms of both species based on polymorphisms were constructed and genetical relationships were established. In rice, half the number of amplified bands were polymorphic and almost all varieties differentiated. However, differentiation of minor genetic alterations among somaclonal variants or mutants and their mother varieties was not feasible. In L. sativa, 47% of the amplified fragments were polymorphic and all 12 varieties were differentiated. Some of the PCR fragments were variety or type specific, which could be used for indicators for type-selection. The dendrogram obtained showed differentiated clusters of crisphead, leaf and butterhead type, findings in good accord with the classification based on the genetic background.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021410
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic variation of mitochondrial and nuclear genomes in carrots revealed by random amplified polymorphic DNA (RAPD) (1997)
    Springer
    Euphytica 95 (1997), S. 259-267 
    Details
    ISSN: 1573-5060
    Keywords: cytoplasmic male sterility ; Daucus carota ; ssp. sativus ; mitochondrial DNA ; pedigree analysis ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Mitochondrial and nuclear genomic diversities of 8 carrot (Daucus carota ssp. sativus) varieties, including 6 pure lines and 2 cytoplasmic male sterile (cms) lines, were taxonomically identified using PCR with 19 RAPD primers. Dendrograms based on polymorphisms of both mitochondrial and nuclear genomes were constructed. According to the dendrogram of the mitochondrial genome revealed by RAPD, 4 differentiated clusters formed, in good accordance with the classification based on analyses with restriction enzyme digestion. Two cms lines were grouped into the same cluster, as genetically separated from the others. Thus, the cytoplasm donors of these male sterile lines were thought to be wild carrots. Conversely, RAPD analysis of the nuclear genome for these eight cultivars revealed no evident clusters although some cultivars were of a similar origin or place of cultivation. A correlation between nuclear and mitochondrial dendrograms was absent. RAPD has proved to be a useful tool for identifying mitochondrial and nuclear genomes. This technique will greatly aid in promoting efficient improvement of carrots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002901112164
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a mutant protoporphyrinogen oxidase gene from Chlamydomonas reinhardtii conferring resistance to porphyric herbicides (1998)
    Springer
    Plant molecular biology 38 (1998), S. 839-859 
    Details
    ISSN: 1573-5028
    Keywords: Chlamydomonas ; complementation ; herbicide resistance ; indexed cosmid library ; nuclear transformation ; protoporphyrinogen oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In plant and algal cells, inhibition of the enzyme protoporphyrinogen oxidase (Protox) by the N-phenyl heterocyclic herbicide S-23142 causes massive protoporphyrin IX accumulation, resulting in membrane deterioration and cell lethality in the light. We have identified a 40.4 kb genomic fragment encoding S-23142 resistance by using transformation to screen an indexed cosmid library made from nuclear DNA of the dominant rs-3 mutant of Chlamydomonas reinhardtii. A 10.0 kb HindIII subclone (Hind10) of this insert yields a high frequency of herbicide-resistant transformants, consistent with frequent non-homologous integration of the complete RS-3 gene. A 3.4 kb XhoI subfragment (Xho3.4) yields rare herbicide-resistant transformants, suggestive of homologous integration of a portion of the coding sequence containing the mutation. Molecular and genetic analysis of the transformants localized the rs-3 mutation conferring S-23142 resistance to the Xho3.4 fragment, which was found to contain five putative exons encoding a protein with identity to the C-terminus of the Arabidopsis Protox enzyme. A cDNA clone containing a 1698 bp ORF that encodes a 563 amino acid peptide with 51% and 53% identity to Arabidopsis and tobacco Protox I, respectively, was isolated from a wild-type C. reinhardtii library. Comparison of the wild-type cDNA sequence with the putative exon sequences present in the mutant Xho3.4 fragment revealed a G→A change at 291 in the first putative exon, resulting in a Val→Met substitution at a conserved position equivalent to Val-389 of the wild-type C. reinhardtii cDNA. A sequence comparison of genomic Hind10 fragments from C. reinhardtii rs-3 and its wild-type progenitor CC-407 showed this G→A change at the equivalent position (5751) within exon 10.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006085026294
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  • 9
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    A single amino-acid substitution in the beta-tubulin gene of Neurospora confers both carbendazim resistance and diethofencarb sensitivity (1992)
    Springer
    Details
    Publication Date: 1992-04-01
    Print ISSN: 0172-8083
    Electronic ISSN: 1432-0983
    Topics: Biology
    Published by Springer
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  • 10
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    Monooxygenase Activity of Saccharomyces cerevisiae Cells Transformed with Expression Plasmids Carrying Rat Cytochrome P-450MC cDNA1 (1986)
    Oxford University Press
    Details
    Publication Date: 1986-01-01
    Print ISSN: 0021-924X
    Electronic ISSN: 1756-2651
    Topics: Biology , Chemistry and Pharmacology
    Published by Oxford University Press on behalf of Japanese Biochemical Society.
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