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Hairy Root Cultures Based Applications : Methods and Protocols (2020)

Srivastava, Vikas. [editor.] ; Mehrotra, Shakti. [editor.] ; Mishra, Sonal. [editor.]

Singapore :Springer Nature Singapore :

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Keywords: Biotechnology. ; Plant diseases. ; Microbial ecology. ; Bacteria. ; Biology Technique. ; Biotechnology. ; Plant Pathology. ; Microbial Ecology. ; Bacteria. ; Biological Techniques.

Description / Table of Contents: Chapter 1. Hairy Roots Biotechnology Unzipped: A Journey of Reality and Promises -- Chapter 2. Hairy Roots as a Source of Tropane Alkaloids -- Chapter 3. Induction, Metabolite Analysis and Transgenesis of Hairy Roots From Coleus Forskohlii -- Chapter 4. Establishment of Hairy Roots of Endangered Himalayan Plantswertiachirata: A Sustainable Alternative to Extraction from Nature -- Chapter 5. A Protocol for the Selection of Spontaneous Variants From Established Catharanthusroseus Hairy Root Cultures -- Chapter 6. Strategies for the Optimization of Culture Conditions for Increasing Metabolite Production Through Hairy Root Cultures. Monoterpenoid Indole Alkaloid Production in Catharanthus Roseus Hairy Roots -- Chapter 7. Protocol for Enhanced Withaferin-a Production in Elicited Withania Somnifera (l.)Dunal Hairy Root Cultures -- Chapter 8. Establishment of Hairy Root Cultures of Pentalinonandrieuxii for the Production of Betulinic Acid -- Chapter 9. Tropane Alkaloid Production by the Establishment Of Hairy Root Cultures of Brugmansia Candida and Elicitation -- Chapter 10. Elicitation as an Essential Strategy For Enhancing Anthraquinone Accumulation In Hairy Root Cultures of Rubia Tinctorum -- Chapter 11. Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion In The Culture Medium -- Chapter 12. An Improved Protocol for Agrobacterium Rhizogenes-Mediated Transformation of Recalcitrant Plants for Root Biology Studies: A Case Study of Tea Plants (Camellia Sinensis Var. Sinensis) -- Chapter 13 Functional Analysis of Plant Genes Related to Arbuscular Mycorrhiza Symbiosis Using Agrobacterium Rhizogenes-Mediated Root Transformation and Hairy Root Production -- Chapter 14. Functional Characterization of Genes Involved in Legume Nodulation Using Hairy Root Cultures -- Chapter 15. Fluidigm Biomark Diagnostic Panel for Analysis of the Expression of Nicotiana Tabacum Genes, Associated with Alkaloid Synthesis. .

Abstract: The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.

Type of Medium: Online Resource

Pages: XIV, 237 p. 47 illus., 39 illus. in color. , online resource.

Edition: 1st ed. 2020.

ISBN: 9789811540554

Series Statement: Rhizosphere Biology,

URL: https://doi.org/10.1007/978-981-15-4055-4

DOI: 10.1007/978-981-15-4055-4

DDC: 660.6

Language: English

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Plant Pathogen Interaction (2023)

Verma, Praveen Kumar. [editor.] ; Mishra, Sonal. [editor.] ; Srivastava, Vikas. [editor.] ; [et al.]

Singapore :Springer Nature Singapore :

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Keywords: Plant diseases. ; Stress (Physiology). ; Plants. ; Plant physiology. ; Plant Pathology. ; Plant Stress Responses. ; Plant Physiology.

Description / Table of Contents: 1. Plant-pathogen interactions studies: Combinatorial approach and multidisciplinary benefits -- 2. Plant-pathogen interactions and global food security -- 3. Fusarium wilt of tomato: past, present, and future -- 4. Late Wilt of Maize—The Pathogen, the Disease, Current Status, and Future Perspective -- 5. Insights into grapevine defence response against fungal and oomycete diseases towards a sustainable plant breeding -- 6. Defensive Strategies of ROS in Plant-Pathogen Interactions -- 7. Phytohormone signaling and Plant-Pathogen Interaction -- 8. Quorum Sensing and its Role in Bacterial Pathogenicity -- 9. Pathobiome and Microbial Community Shifts Associated with Vegetable, Fruit and Cereal Crops -- 10. Prevalence of microbiome reservoirs in plants and pathogen outbreaks -- 11.Exploring Plant-Pathogen Interactions through Subcellular Proteomics -- 12. Genome Editing and Plant-Pathogen Interaction -- 13. Secreted effectors: a perspective in plant-fungus interaction.

Abstract: This book covers all aspects of naturally occurring phenomenon of Plant-Pathogen Interaction (PPI). Recent findings and scientific explanations to understand PPI are provided accompanied by numerous helpful photographs and pictorial presentations. In addition, tabulated data is also included to aid in getting insight into the subject and identifying the missing links. Essential information is provided on physiological, biochemical and pathology consequences of PPI and distinguished sections are devoted to explain molecular and regulatory mechanism underlying PPI. Further topics include different classes of plant pathogen, receptor molecules, signaling system, secondary metabolism and plant defense system etc. This book helps the readers in understanding the state of art and emerging technics to explore PPI and in identifying the missing links which further help in creating the background for future exploration of PPI in terms of experimental and technical advancements. .

Type of Medium: Online Resource

Pages: XV, 362 p. 1 illus. , online resource.

Edition: 1st ed. 2023.

ISBN: 9789819948901

URL: https://doi.org/10.1007/978-981-99-4890-1

DOI: 10.1007/978-981-99-4890-1

DDC: 571.92

Language: English

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Tropane Alkaloids : Pathways, Potential and Biotechnological Applications (2021)

Srivastava, Vikas. [editor.] ; Mehrotra, Shakti. [editor.] ; Mishra, Sonal. [editor.]

Singapore :Springer Nature Singapore :

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Keywords: Biotechnology. ; Botanical chemistry. ; Pharmacology. ; Plant physiology. ; Biotechnology. ; Plant Biochemistry. ; Pharmacology. ; Plant Physiology.

Description / Table of Contents: Part 1. Tropane Alkaloids: Diversity, Biosynthesis and Significance -- Chapter 1. Plant Tropane Alkaloids (TAs): Chemotaxonomic Significance, Biogenesis and Biotechnological interventions -- Chapter 2. Structure and Function of Enzymes Involved in the Biosynthesis of Tropane Alkaloids -- Chapter 3. Plant tropane alkaloids: Commercial stature and production developments -- Part 2. Tropane Alkaloids: In vitro and allied interventions -- Chapter 4. Involvement of various biotechnological contrivances for tropane alkaloids biosynthesis and applications of tropane alkaloid bearing in vitro cultures -- Chapter 5. Biotechnological Production of Tropane Alkaloids: Contribution of Hairy Root Cultures -- Chapter 6. Production of Tropane Alkaloids (TAs) in plant and in vitro cultures of different ploidy levels -- Chapter 7. Current Progress in Tropane Alkaloids Production by Transgenic and Heterologous gene Expression Approaches -- Chapter 8. Tropane Alkaloid Biosynthesis in Plants: Insights from Transcriptome Analysis -- Chapter 9. Transcription Factor and MicroRNA-mediated Manipulation of the Tropane Alkaloids Biosynthesis. .

Abstract: This book covers the current research on the plant-based tropane alkaloids (TA) and TA-bearing plants. The first part includes an overview of “tropane alkaloids in context to diversity, biosynthesis and significance. The second part covers aspects of in-vitro and allied interventions in these alkaloids. TAs belong to one of the world’s oldest phytomedicines with their valuable ethno-pharmacological applications. The book compiles scientific ideas, perspectives and challenges in this area. Further, the book attempts to investigate the loopholes in existing methodologies, challenges and possible solutions through well thought-out scientific discussions from various eminent research groups. This book is of high interest to students and researchers in biotechnology, plant biology and pharmacology. As the TAs are of high commercial medicinal value, this book is also a relevant collection of articles for industry experts.

Type of Medium: Online Resource

Pages: XIV, 172 p. 20 illus., 13 illus. in color. , online resource.

Edition: 1st ed. 2021.

ISBN: 9789813345355

URL: https://doi.org/10.1007/978-981-33-4535-5

DOI: 10.1007/978-981-33-4535-5

DDC: 660.6

Language: English

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