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  • 1
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 41 (1986), S. 17-24 
    ISSN: 1570-7458
    Keywords: Schizaphis graminum ; Myzus persicae ; Acyrthosiphon pisum ; host-plant resistance ; pectin ; aphid-probing behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Drei Blattlausarten, Schizaphis graminum, Acyrthosiphon pisum and Myzus persicae, zeigten deutliche Unterschiede in ihrem Probeverhalten auf künstlichen Nährmedien, die mit dünnen (〈200 μm), verschiedene Mono- und Polysaccharide enthaltenden Agarschichten bedeckt waren. Die in den Agarschichten enthaltenen Monosaccharide bewirkten bei allen drei Arten eine deutliche Steigerung des Probeverhaltens. Im Gegensatz dazu war der Einfluss der Polysaccharide von Art zu Art verschieden. Sechs von sieben getesteten pflanzlichen Matrixpolysacchariden beeinflussten deutlich das Probeverhalten von Schizaphis graminum und Acyrthosiphon pisum. Im Gegensatz dazu wurde das Probeverhalten von Myzus persicae nur von einem der untersuchten Polysaccharide beeinflusst. Die Zahl der Wirtspflanzen für Schizaphis graminum und Acyrthosiphon pisum ist relativ klein im Vergleich zu der von Myzus persicae. Dies ist möglicherweise darauf zurückzuführen, daß die Fähigkeit, chemosensorisch zwischen pflanzlichen Matrixpolysacchariden zu unterscheiden, bei Myzus persicae weniger stark ausgeprägt ist. Elektronische Registrierung des Probeverhaltens zeigte, daß Schizaphis graminum vor der Aufnahme der künstlichen Nährmedien zunächst die darüber liegenden Agarschichten testete. Dies geschah durch Einstechen des Stiletts in die Agarschicht, Absonderung von Speichel, Aufsaugen der verflüssigten Matrix und auschliessendes Wiederausstoßen. Das Probeverhalten von Schizaphis graminum auf Trifluoroacetylpektin, welches eine Aufnahme der darunter liegenden Nährstoffe verhinderte, war vergleichbar zu dem Probeverhalten von Schizaphis Graminum auf resistenten oder Nichtwirtspflanzen. Andererseits glich das Probeverhalten von Schizaphis graminum stark methyliertem Pektin, welche die Nährstoffaufnahme steigerte, dem auf natürlichen Wirtspflanzen. Die Ergebnisse dieser Untersuchung zeigen, daß pflanzliche Matrixpolysaccharide für die Wechselwirkung zwischen Blattläusen und Pflanzen keine chemisch inerten Komponenten darstellen. Der unterschiedliche Einfluss der pflanzlichen Matrixpolysaccharide auf die verschiedenen Blattlausarten zeigt, daß diese pflanzlichen Biopolymere eine entscheidene Rolle bei der Erkennung von Wirtspflanzen durch Blattläuse spielen könnten. Darüberhinaus zeigt das extrem unterschiedliche Probeverhalten von Schizaphis graminum auf Trifluoroacetyl- bzw. stark methyliertem Pektin, daß selbst geringe Änderungen in den chemischen Eigenschaften dieser Komponenten das Probeverhalten von Blattläusen deutlich beeinflussen können.
    Notes: Abstract Three species of aphids, Schizaphis graminum (Rondani) (the greenbug), Acyrthosiphon pisum (Harris) (the pea aphid) and Myzus persicae (Sulzer) (the green peach aphid), showed significantly different behavioral responses to various plant mono- or polysaccharides incorporated within thin (〈200 μm) films of agar overlaying artificial diets. Thirteen polysaccharides (i.e.: polygalacturonic acid, low methoxy pectin, high methoxy pectin, arabinogalactan, xylan, galactan, cellulose, starch, amylose, laminarin, dextran, 2,3-diacetyl pectin and trifluoroacetyl pectin) and 5 monosaccharides (arabinose, xylose, galactose, glucose and galacturonic acid) were tested in paired choices against a control overlay containing only agar. Glucose, dextran and laminarin were the only compounds that all three species of aphids responded to similarly (viz., glucose stimulated, dextran had no effect and laminarin deterred ingestion of underlying diets). None of the aphids exhibited negative responses to the monosaccharides tested. These monosaccharides either had no significant effect or were stimulatory. The polysaccharides tested induced a variation of unaltered, stimulatory or inhibitory behavioral responses in the 3 species of aphids. The behavioral responses of both greenbugs and pea aphids, which are oligophagous, were significantly affected by 6 out of 7 plant matrix polysaccharides tested. Feeding behavior by green peach aphids was affected by only one of these polysaccharides. Electronic monitoring of aphid probing showed that aphids salivated in and ‘test’ probed the overlays prior to inserting their stylets into and ingesting from the underlying diets. Recorded responses of aphids probing overlays containing inhibitory polysaccharides resembled the recorded behavioral responses of aphids on resistant or nonhost plants (i.e., numerous short probes with either a lack of or a prolonged period prior to ingestion). The differential behavioral responses of the aphids to the various polysaccharides supports the view that plant matrix polysaccharides play a role in the chemical basis of aphid — plant interactions.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 44 (1987), S. 229-234 
    ISSN: 1570-7458
    Keywords: feeding deterrents ; Schizaphis graminum ; Acyrthosiphon pisum ; Myzus persicae ; Homoptera ; Aphididae ; ellagitannins ; phenolics ; gallic acid derivatives ; structure-activity relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'examen a porté sur l'action répulsive, lors de l'alimentation de trois espèces de pucerons, de l'acide ellgique, de deux ellagitanins, de l'acide gallique, du pyrogallol et de plusieurs dérivés de l'acide gallique. Schizaphis graminum Rondani a été l'espèce la plus sensible, tandis que Acyrthosiphon pisum. Harris a été la moins sensible; la sensibilité de Myzus persicae Sulzer était intermédiaire. L'acide ellagic (ED50=15 ppm) et le n-décyl gallate (ED50=16 ppm) ont été particulièrement actifs contre S. graminum, tandis que le n-octyl gallate a été le produit le plus actif contre A. pisum (ED50=182 ppm) et M. persicae (ED50=56 ppm). Les ellagitanins, géraniine et pédunculagine ont été actifs contre S. graminum et M. persicae, mais pas contre A. pisum. La méthylation des groupes hydroxyl libres de l'acide gallique a réduit fortement l'activité, tandis qu l'estérification de son groupe carboxyl avec des chaînes alkyl de longuers croissantes a augmenté l'activité contre S. graminum. Les acides ellagique, gallique et 3,4,5-triméthoxybenzoïque ont été inactifs contre A. pisum et M. persicae, tandis que le pyrogallol et les esters de gallate on été pour le moins des répulsifs modérément actifs au cours de l'alimentation.
    Notes: Abstract Feeding deterrent activities of ellagic acid, two ellagitannins, gallic acid, pyrogallol, and several gallic acid derivatives towards three species of aphids were determined. The most sensitive species tested was Schizaphis graminum (Rondani), the least sensitive was Acyrthosiphon pisum (Harris). Myzus persicae (Sulzer) was of intermediate sensitivity. Ellagic acid (ED50=15 ppm) and n-decyl gallate (ED50=16 ppm) were particularly potent against S. graminum, while n-octyl gallate was the most active compound tested against A. pisum (ED50=182 ppm) and M. persicae (ED50=56 ppm). The ellagitannins, geraniin and pedunculagin, were active against S. graminum and M. persicae, but not against A. pisum. Methylation of the free hydroxyl groups of gallic acid resulted in a large decrease in activity, while esterification of its carboxyl group with alkyl chains of increasing length resulted in increasing activity against S. graminum. Against A. pisum and M. persicae, ellagic acid, gallic acid and 3,4,5-trimethoxybenzoic acid were inactive, whereas pyrogallol and the gallate esters were at least moderately active as feeding deterrents.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 78 (1968), S. 366-370 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Gibberellin A14 and kaurene, precursors of gibberellin A3, are active in the barley endosperm reducing-sugar assay, but require longer incubation periods for activity to be observed than does GA3. This finding shows that the incubation period must be considered when determining whether a compound is active in this assay. The longer incubation periods required by GA14 and kaurene may reflect reduced rates of penetration, or reduced activity within the cell, or the time required for conversion to a physiologically active form.
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  • 5
    ISSN: 1573-1561
    Keywords: Medicago sauva ; alfalfa ; Hypera brunneipennis ; H. postica ; Coleoptera ; Curculionidae ; alfalfa weevil ; host-plant resistance ; bioassay ; feeding deterrency ; ovicide ; antigrowth ; coumarins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A resistant line of alfalfa was studied in an attempt to identify possible chemical factors responsible for alfalfa weevil (Hypera brunneipennis) resistance. Bioassays were developed to measure ovicidal, larval development, pupation, and adult development rates. Serial alfalfa extracts of a weevil-resistant line showed no effect in the bioassays when compared to those of a weevil-susceptible line. It was concluded that resistance substances are not present but that resistant lines are simply more tolerant to weevil attack. Alfalfa constitutents and synthetic analogs were also screened in the egg hatch bioassay. Among these were a series of 3-acyl-4-hydroxycoumarins. 3-Acyl-4-hydroxycoumarins with short-chain acyl groups showed good ovicidal rates, but activity decreased with increasing length of the acyl group.
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  • 6
    ISSN: 1573-1561
    Keywords: Feeding deterrents ; Acyrthosiphon pisum ; Homoptera ; Aphi-didae ; host-plant resistance ; pyrrolizidine ; indolizidine ; quinolizidine ; alkaloids ; locoweed ; honeydew ; Astragalus lentiginosus ; phloem transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The feeding deterrency of a series of pyrrolizidine, indolizidine, and quinolizidine alkaloids and selected derivatives was measured against the pea aphid (Acyrthosiphon pisum Harris). The indolizidine alkaloid, castanospermine, was intensely active (ED50, 20 ppm) as were the quinolizidine alkaloids, but only modest feeding deterrency was observed with most of the pyrrolizidine alkaloids tested. The insect survival rate of aphids on a castanospermine-supplemented diet over 24 hr was also very low relative to the controls. Castanospermine does not inhibit aphid trehalase. The indolizidine alkaloid swainsonine occurred in the honeydew of pea aphid feeding on the locoweed,Astragalus lentiginosus. Since the pea aphid is a phloem feeder, swainsonine must be transported in the phloem.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 7 (1981), S. 273-284 
    ISSN: 1573-1561
    Keywords: Schizaphis graminum ; Homoptera ; Aphididae ; greenbugs ; Sorghum bicolor ; feeding deterrents ; bioassay ; aphids ; host plant resistance ; p-hydroxybenzaldehyde ; dhurrin ; procyanidin ; luteolin-7-glucoside
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Improvements in a synthetic diet for use in a bioassay to screen for feeding deterrents againstSchizaphis graminum, greenbug, are reported. Feeding on the synthetic diet was highly pH dependent with maximum feeding occurring at about pH 8.0. The bioassay was used as a guide in the isolation of feeding deterrent substances from aphid-resistant lines of sorghum (Sorghum bicolor). The major greenbug feeding deterrents isolated from sorghum leaves wereP-hydroxybenzaldehyde (ED50 0.13%), dhurrin (ED50 0.16%), and procyanidin (ED50 0.08%).
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  • 8
    ISSN: 1573-1561
    Keywords: Medicago saliva ; alfalfa ; Acyrthosiphon pisum ; pea aphid ; Homoptera ; Aphididae ; host-plant resistance ; phytoalexin ; 4-hydroxycoumarins ; coumestrol ; symbiotes ; feeding deterrency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A series of 3-acyl-4-hydroxycoumarins, structurally related to dicoumarol, as well as several alfalfa constituents including coumestrol were tested for their feeding deterrency towards the pea aphid. Feeding deterrency of the 3-acyl-4-hydroxycoumarins decreased as the size of the 3-acyl group increased.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 13 (1987), S. 1759-1770 
    ISSN: 1573-1561
    Keywords: Castanospermine ; 6-epicastanospermine ; deoxynojirimycin ; cellobiase ; lactase ; maltase ; sucrase ; trehalase ; endosymbiote ; insect-plant interactions ; allelochemicals ; Homoptera ; Coleoptera ; Lepidoptera ; Diptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The indolizidine alkaloid, castanospermine (1,6,7,8-tetrahydroxy-octahydroindolizidine—a stereochemical mimic of glucose found in the Australian legumeCastanospermum australe), differentially inhibited cellobiose, lactose, maltose, sucrose, and trehalose hydrolyzing enzymes from a broad taxonomic spectrum of insects (19 species from 12 different families). It was a potent inhibitor of cellobiase activity of all insects tested (50% inhibition at 〈3.2 × 10−5 M castanospermine). With one exception, it also inhibited lactase activity of all insects examined. Only in the sap-feeding Homoptera did castanospermine inhibit all disaccharidase activities assayed. Trehalase activity of the Lepidoptera and Diptera was generally inhibited by castanospermine, whereas inhibition of trehalase activity of the Coleoptera by castanospermine was exiguous or not detectable. Castanospermine was a significant feeding deterrent towards pea aphids,Acyrthosiphon pisum, with an ED50 of 1 × 10−4 M in artificial diets. Two compounds stereochemically related to castanospermine, deoxynojirimycin and 6-epicastanospermine, were each slightly active at deterring the feeding of green peach aphids,Myzus persicae, (ED50=2.5 × 10−3 M) and greenbugs,Schizaphis graminum (ED50=5 × 10−3 M), respectively. Among the insects studied there was no distinct relationship between enzyme inhibition and adaptation to host plants containing castanospermine or other toxic alkaloids.
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  • 10
    Publication Date: 1968-02-01
    Print ISSN: 0031-9422
    Electronic ISSN: 1873-3700
    Topics: Biology , Chemistry and Pharmacology
    Published by Elsevier
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