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  • 1
    ISSN: 1573-7632
    Keywords: metabolome ; tomato fruit ; salinity ; Fourier transform infra-spectroscopy (FTIR) ; chemometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Genetic programming, in conjunction with advanced analytical instruments, is a novel tool for the investigation of complex biological systems at the whole-tissue level. In this study, samples from tomato fruit grown hydroponically under both high- and low-salt conditions were analysed using Fourier-transform infrared spectroscopy (FTIR), with the aim of identifying spectral and biochemical features linked to salinity in the growth environment. FTIR spectra of whole tissue extracts are not amenable to direct visual analysis, so numerical modelling methods were used to generate models capable of classifying the samples based on their spectral characteristics. Genetic programming (GP) provided models with a better prediction accuracy to the conventional data modelling methods used, whilst being much easier to interpret in terms of the variables used. Examination of the GP-derived models showed that there were a small number of spectral regions that were consistently being used. In particular, the spectral region containing absorbances potentially due to a cyanide/nitrile functional group was identified as discriminatory. The explanatory power of the GP models enabled a chemical interpretation of the biochemical differences to be proposed. The combination of FTIR and GP is therefore a powerful and novel analytical tool that, in this study, improves our understanding of the biochemistry of salt tolerance in tomato plants.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5087
    Keywords: gas chromatography ; mass spectrometry ; 1-aminocyclopropane-1-carboxylic acid ; 1-(malonylamino)cyclopropane-1-carboxylic acid ; Lycopersicon esculentum Mill.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since the discovery of1-(malonylamino)cyclopropane-1-carboxylic acid (MACC)as a major metabolite of both endogenous andexogenously applied 1-aminocyclopropane-1-carboxylicacid (ACC), it has become evident that the formationof MACC from ACC can act to regulate ethyleneproduction in certain tissues. Hence it was suggestedthat MACC could serve as an indicator of water-stresshistory in plant tissues. The accurate quantificationof MACC in plant tissues is essential forunderstanding the role of MACC in the regulation ofethylene biosynthesis.Hoffman et al. [15] described a method for themeasurement of MACC in which MACC was hydrolysed byHCl to ACC, which was then assayed by chemicaloxidation to form ethylene. Attempts have been made byothers to raise monoclonal antibodies to MACC so thatan immunoassay could be developed in order to gain adeeper understanding of stress-induced ethyleneproduction but no further publications have beenforthcoming.Here a method employing GC-MS is compared with theindirect assay for MACC, which is based uponhydrolysis of MACC to ACC and conversion of ACC byhypochlorite reagent to ethylene which is subsequentlyquantified by GC.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2022-05-25
    Description: Author Posting. © American Geophysical Union, 2005. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Paleoceanography 20 (2005): PA3009, doi:10.1029/2004PA001091.
    Description: Similar orbital geometry and greenhouse gas concentrations during marine isotope stage 11 (MIS 11) and the Holocene make stage 11 perhaps the best geological analogue period for the natural development of the present interglacial climate. Results of a detailed study of core MD01-2443 from the Iberian margin suggest that sea surface conditions during stage 11 were not significantly different from those observed during the elapsed portion of the Holocene. Peak interglacial conditions during stage 11 lasted nearly 18 kyr, indicating a Holocene unperturbed by human activity might last an additional 6–7 kyr. A comparison of sea surface temperatures (SST) derived from planktonic foraminifera for all interglacial intervals of the last million years reveals that warm temperatures during peak interglacials MIS 1, 5e, and 11 were higher on the Iberian margin than during substage 7e and most of 9e. The SST results are supported by heavier δ18O values, particularly during 7e, indicating colder SSTs and a larger residual ice volume. Benthic δ13C results provide evidence of a strong influence of North Atlantic Deep Water at greater depths than present during MIS 11. The progressive ocean climate deterioration into the following glaciation is associated with an increase in local upwelling intensity, interspersed by periodic cold episodes due to ice-rafting events occurring in the North Atlantic.
    Description: This work was partially undertaken in association with the ‘‘POP Project,’’ EC grant EVK2-2000-00089. Funding for L.A. was provided by the Portuguese Foundation for Science and Technology under the fellowship contract SFRH/BPD/1588/2000 and by the Calouste Gulbenkian Foundation, through a visiting fellowship to Woods Hole Oceanographic Institution.
    Keywords: Stage 11 ; Interglacials ; Planktonic foraminifera ; Stable isotopes ; Western Iberian margin ; Eastern North Atlantic
    Repository Name: Woods Hole Open Access Server
    Type: Article
    Format: application/pdf
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  • 4
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    PANGAEA
    In:  Supplement to: Pisias, Nicklas G; Shackleton, Nicholas J; Hall, Michael A (1985): Stable isotope and calcium carbonate records from hydraulic piston cored Hole 574A: high-resolution records from the middle Miocene. In: Mayer, L; Theyer, E; et al. (eds.), Initial Reports of the Deep Sea Drilling Project, Washington (U.S. Govt. Printing Office), 85, 735-748, https://doi.org/10.2973/dsdp.proc.85.121.1985
    Publication Date: 2023-05-12
    Description: Detailed stable isotopic and calcium carbonate records (with a sampling resolution of 3000 yr.) from the middle Miocene section of hydraulic piston corer (HPC) Hole 574A provide a sequence that records the major shift in the oxygen isotopic composition of the world's oceans that occurred at about 14 Ma. The data suggest that this transition was rapid and spans about 30,000 yr. of sediment deposition. In intervals before and after the shift, the mean d18O values are characterized by a constant mean with a high degree of variability. The degree of variability in both the d18O and d13C records is comparable to that observed for the Pliocene and earliest Pleistocene and does not show a significant change before or after the major shift in the d18O record. Whereas the oxygen isotopic record is characterized by relatively stable mean values before and after the middle Miocene event, the d13C record shows a number of significant offsets in the mean value separated by intervals of high-frequency variations. Time and frequency domain analysis of all records from Hole 574A indicate that the frequency components shown to be related to orbital changes in the Pleistocene record are also present in the middle Miocene. The high variability observed in the Site 574 isotopic records places important constraints on models describing the role of formation of the Antarctic ice sheet during the middle Miocene climatic transitions. Thus, HPC Hole 574A provides a valuable sequence for detailed study of climatic variability during an important time in the Earth's history, although we cannot provide a definitive explanation of the major oxygen isotopic event of the middle Miocene.
    Keywords: Deep Sea Drilling Project; DSDP
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 5
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    In:  Supplement to: Ferretti, Patrizia; Crowhurst, Simon J; Hall, Michael A; Cacho, Isabel (2010): North Atlantic millennial-scale climate variability 910 to 790 ka and the role of the equatorial insolation forcing. Earth and Planetary Science Letters, 293(1-2), 28-41, https://doi.org/10.1016/j.epsl.2010.02.016
    Publication Date: 2023-05-12
    Description: The Mid-Pleistocene transition (MPT) was the time when quasi-periodic (? 100 kyr), high-amplitude glacial variability developed in the absence of any significant change in the character of orbital forcing, leading to the establishment of the characteristic pattern of late Pleistocene climate variability. It has long been known that the interval around 900 ka stands out as a critical point of the MPT, when major glaciations started occurring most notably in the northern hemisphere. Here we examine the record of climatic conditions during this significant interval, using high-resolution stable isotope records from benthic and planktonic foraminifera from a sediment core in the North Atlantic (Integrated Ocean Drilling Program Expedition 306, Site U1313). We have considered the time interval from late in Marine Isotope Stage (MIS) 23 to MIS 20 (910 to 790 ka). Our data indicate that interglacial MIS 21 was a climatically unstable period and was broken into four interstadial periods, which have been identified and correlated across the North Atlantic region. These extra peaks tend to contradict previous studies that interpreted the MIS 21 variability as consisting essentially of a linear response to cyclical changes in orbital parameters. Cooling events in the surface record during MIS 21 were associated with low benthic carbon isotope excursions, suggesting a coupling between surface temperature changes and the strength of the Atlantic meridional overturning circulation. Time series analysis performed on the whole interval indicates that benthic and planktonic oxygen isotopes have significant concentrations of spectral power centered on periods of 10.7 kyr and 6 kyr, which is in agreement with the second and forth harmonic of precession. The excellent correspondence between the foraminifera d18O records and insolation variations at the Equator in March and September suggests that a mechanism related to low-latitude precession variations, advected to the high latitudes by tropical convective processes, might have generated such a response. This scenario accounts for the presence of oscillations at frequencies equal to precession harmonics at Site U1313, as well as the occurrence of higher amplitude oscillations between the MIS22/21 transition and most of MIS 21, times of enhanced insolation variability.
    Keywords: Integrated Ocean Drilling Program / International Ocean Discovery Program; IODP
    Type: Dataset
    Format: application/zip, 2 datasets
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  • 6
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    In:  Supplement to: Shackleton, Nicholas J; Hall, Michael A; Pate, D; Meynadier, Laure M; Valet, P (1993): High-resolution stable isotope stratigraphy from bulk sediment. Paleoceanography, 8(2), 141-148, https://doi.org/10.1029/93PA00123
    Publication Date: 2023-05-12
    Description: A high-quality delta18O record has been obtained from a deep sea core in the Somali Basin in a water depth of 4020 m, well below the foraminiferal lysocline, by analyzing bulk sediment instead of picked foraminifera. In some areas this may be a valuable tool for extending the applicability of the delta18O stratigraphy, for obtaining data by a less labor-intensive procedure, and for conserving valuable core material. Additionally, we draw attention to the need for more data on delta13C in bulk carbonate if we are to fully utilize the ocean delta13C record either in relation to long-term global carbon budget, or in relation to changes in carbon cycling in the ocean and its effect on atmospheric pCO2.
    Keywords: DEPTH, sediment/rock; Marion Dufresne (1972); Mass spectrometer VG Isogas Prism; MD_1985; MD85-668; PC; Piston corer; δ13C, carbonate; δ18O, carbonate
    Type: Dataset
    Format: text/tab-separated-values, 516 data points
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  • 7
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    In:  Supplement to: Boersma, Anne; Shackleton, Nicholas J; Hall, Michael A; Given, Quentin (1979): Carbon and oxygen isotope records at DSDP Site 384 (North Atlantic) and some Paleocene paleotemperatures and carbon isotope variations in the Atlantic Ocean. In: Tucholke, B.E., Vogt, P.R., et al. (eds.), Initial Reports of the Deep Sea Drilling Project, Washington (U.S. Government Print Office), 43, 695-717, https://doi.org/10.2973/dsdp.proc.43.131.1979
    Publication Date: 2023-06-27
    Description: Detailed analysis of over 200 samples of uppermost Cretaceous and Paleocene sediments from Atlantic Ocean DSDP Sites 384, 86, 95, 152, 144, 20C, 21, 356, 357, and 329 provides new information on the temperature stratification of Paleocene planktonic foraminifera, the temperature and carbon isotopic changes across the Cretaceous/Tertiary boundary, and the fluctuating temperature and carbon isotopic records through the Paleocene ~64.5-54 m.y.). There was a significant temperature rise across the Cretaceous/Tertiary boundary both at the surface and in deep waters of the Atlantic Ocean. This temperature rise occurred before the basal Tertiary 'Globigerina' eugubina Zone, so that in the oldest Paleocene sample yet analyzed from the deep sea (Site 356) temperatures are already three degrees higher at the bottom and at the surface than in the Cretaceous. The temperature rise across the boundaryis more pronounced on the bottom and in samples from higher latitudes. Accompanying the temperature rise across the boundary there is a significant shift in the carbon isotope profile. In the basal Paleocene the foraminifera of the surface zone demonstrate very negative carbon isotope values (unlike in the Cretaceous of today's ocean), while deeper dwelling species have more positive values which then decrease to the bottom. The unusual carbon isotope gradients persist through the first three million years of the Paleocene until towards the top of planktonic foraminiferal Zone P.1 (G. trinidadensis Zone) the foraminifera record a profile more positive at the surface and decreasing towards the bottom (as in today's ocean). During the Paleocene there are two noteworthy rises in surface water temperature; the first around 62-61 m.y. (G. trinidadensis Zone), and the second near the base of the Globorotalia angulata Zone, 60-59 m.y. At this time surface temperatures at low to mid latitudes reached values near 25°C, while at mid-latitude Site 384 temperature highs near 22°C were registered. At a sample spacing of around one per million years, we have only produced some of the detail of these temperature fluctuations. The later Paleocene is generally cooler and there do not seem to be any large variations either through time or latitude. Middle-latitude sites average temperatures near 15°C at the surface, while high lower latitude site temperatures range near 18°C. The most salient feature of the bottom temperature record (based on multispecific samples) through the Paleocene is its lack of fluctuations. There is an overall temperature range of 5°C at these intermediate depth sites (paleodepth estimates between 1500 and 3000 m). Higher values near 13°C accompany the surface temperature peaks around 62 and 60 m.y., while low values near 8°C occur in Zone P.2 (61-60 m.y.). We detected no change in bottom temperature across the paleocene/Eocene boundary in the few samples studied so far. While there are several fluctuations in the carbon isotope values through the early Paleocene, the general trend is one of increasingly positive values at the surface and at depth. This trend culminates in the late Paleocene (upper Zone P.4, about 56-57 m.y.) with a major excursion in the carbon isotope values. At low latitudes the range between the surface and the deepest planktonic foraminifera is a delta13C of 4 per mil as compared with a range of 2 per mil today. The carbon values drop off slightly, but remain strongly positive through the remainder of the Paleocene at most sites. Accompanying the carbon isotope excursion at Site 384 is a productivity increase and a proposed rise in the CCD.
    Keywords: 10-86; 10-94; 10-95; 14-144A; 3-20C; 3-21; 39-356; 39-357; 43-384; 63-472; Deep Sea Drilling Project; DRILL; Drilling/drill rig; DSDP; Glomar Challenger; Gulf of Mexico/BENCH; Gulf of Mexico/SCARP; Leg10; Leg14; Leg3; Leg39; Leg43; Leg63; North Atlantic/CONT RISE; North Atlantic/RIDGE; North Pacific/PLATEAU; South Atlantic/CONT RISE; South Atlantic/PLATEAU; South Atlantic/VALLEY
    Type: Dataset
    Format: application/zip, 5 datasets
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  • 8
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    In:  Supplement to: de Abreu, Lucia; Shackleton, Nicholas J; Schönfeld, Joachim; Hall, Michael A; Chapman, Mark R (2003): Millenial-scale oceanic climate variability off the Western Iberian margin during the last two glacial periods. Marine Geology, 196(1-2), 1-20, https://doi.org/10.1016/S0025-3227(03)00046-X
    Publication Date: 2023-06-27
    Description: High-resolution palaeoclimate records recovered from the Iberian margin in core MD95-2040 exhibit large fluctuations in oceanographic conditions over the last 190 ka. Large-scale cooling of the surface ocean is indicated by the presence of the polar planktonic foraminifer Neogloboquadrina pachyderma (sinistral), and in some instances the occurrence of ice-rafted debris (IRD). Ice-rafting episodes were prevalent in both of the last two glacials with greater intensity in Stages 2 through 4, than in Stage 6. The six youngest Heinrich events are well defined during the last glacial but detrital carbonate is absent from Heinrich layers HL6, HL5 and HL3. Dansgaard-Oeschger stadial-equivalent sub-millennial IRD deposition events have been detected, in particular during Stage 3, allowing a good match with the cooling displayed in the Greenland ice core (GISP2). Sea-surface temperature off Portugal in Stage 6 was in general warmer than during the last glacial, pointing towards a weaker southward influence of polar water masses. Ice rafting occurred mainly in mid-MIS (Marine Isotope Stage) 6 (between 173 and 153 kyr) as a group of poorly differentiated, short-duration quasi-continuous events, mainly marked by the high abundance of sinistral N. pachyderma. Differences exist in IRD composition relative to the last glacial, with a reduced Canadian-derived detrital carbonate component, combined with an important contribution of volcanic particles. The lower magnitude and higher frequency of these events suggest that the higher temperatures would have induced iceberg waning closer to the source areas.
    Keywords: CALYPSO; Calypso Corer; IMAGES; IMAGES I; International Marine Global Change Study; Marion Dufresne (1995); MD101; MD952040; MD95-2040; Porto Seamount
    Type: Dataset
    Format: application/zip, 3 datasets
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  • 9
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    In:  Supplement to: Shackleton, Nicholas J; Hall, Michael A; Boersma, Anne (1984): Oxygen and carbon isotope data from Leg 74 foraminifers. In: Moore, TC Jr; Rabinowitz, PD; et al. (eds.), Initial Reports of the Deep Sea Drilling Project (U.S. Govt. Printing Office), 74, 599-612, https://doi.org/10.2973/dsdp.proc.74.115.1984
    Publication Date: 2023-06-27
    Description: Oxygen and carbon isotope measurements have been made in picked planktonic and benthonic foraminifers from the five sites drilled on Leg 74, covering the whole Cenozoic. For the Neogene, the coverage gives good information on the development of the vertical temperature structure of Atlantic deep water. For the Paleogene, vertical gradients were weak and it is possible to combine data from different sites to obtain a very detailed record of both the temperature and carbon isotope history of Atlantic deep waters.
    Keywords: 74-525A; 74-525B; 74-526A; 74-526B; 74-527; 74-528; 74-528A; 74-529; Deep Sea Drilling Project; DRILL; Drilling/drill rig; DSDP; Glomar Challenger; Leg74; South Atlantic; South Atlantic/CREST; South Atlantic/RIDGE; South Atlantic/SLOPE
    Type: Dataset
    Format: application/zip, 3 datasets
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  • 10
    Publication Date: 2023-06-27
    Keywords: 81-552A; Calcium carbonate; Deep Sea Drilling Project; DEPTH, sediment/rock; DRILL; Drilling/drill rig; DSDP; DSDP/ODP/IODP sample designation; Glomar Challenger; Leg81; North Atlantic/PLATEAU; Sample code/label
    Type: Dataset
    Format: text/tab-separated-values, 1108 data points
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