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  • 1
    Publication Date: 2021-05-19
    Description: Chitin nanofibers are prepared from the exoskeletons of shrimp by a simple mechanical treatment after a series of purification steps. The nanofibers have fine nanofibers networks with a uniform width of approximately 10 nm. Grinders and highpressure water jet systems are effective for disintegrating chitin into nanofibers. Acidic conditions are the key factor to facilitate mechanical fibrillation. Ultrafine fibers were successfully fabricated chitosan and fish skinextracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofibers. Due to the poor mechanical properties of the fish gelatin membranes, composite nanofibers made of fish gelatin and poly(L-lactide)(PLLA) were produced with a novel solution. The introduction of PLLA remarkably improved the mechanical properties of the gelatin membranes. With a combination of good biocompatibility and mechanical properties, fish gelatin/PLGA blending non-woven mats are considered to be very promising in fish fillet coating application.. in this study, we fabricated a novel nanofibers composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibers were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreases as FC content was increased in the FC/PCL composite nanofibers. Several modifications to the chitin NF surface are achieved, including acetylation, deacetylation and maleylation. The results of this study revealed that: 1 –It is possible to produce Nanofibers from chitosan and fish gelatin. 2 – Covering and coating of processed fish by nanofibers are applicable and increasing the possibility of shell life for the processed fish. 3 – Nanofibers which have been produced from chitosan and fish gelation not only is environmentally friendly but also it will be eatable while has been covered for fish fillets. biocompatible chitosan and gelatin made from fish, fresh fish fillets do not have the ability to cover and packaging, but is edible and used.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chitin ; Chitosan ; Nanofiber ; Chemical modification ; Fish ; Aquatic ; Maintenance
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: Babolrood Hydrological measurements are essential for the interpretation of water quality data and for water resource management. Variations in Babolrood hydrological conditions have important effects on water quality. In rivers, such factors as the discharge (volume of water passing through across-section of the river in a unit of time), the velocity of flow, turbulence and depth will influence water quality. Location and geographic point of wetlands Babolrod River: 36°41'21"N 52°36'1"E. Babolrood Is a river in Mazandaran Province, northern Iran that flows northward from foot of Mount Alborz through Babolsar city, and finally to the Caspian Sea between east Babolsar and west Babolsar. Babolrod is a very suitable river for fish to spawn and breed. This has the result of emphasizing Babolrood hydrology regime of the river flood discharge and precipitations are directly dependent on the amount of water Debbie. Results of studies done on this river also confirmed Hydro biology would employ four special biological area D and C and B and A (Biological in divided zoon) Babolrood in the ruling. Area A: Length of about 4 km from estuary of the river there is containing freshwater and brackish And specific biological markers in this region is the general slope of 1-0% in the coastal region. Area B: Length of about 16 Km (20 Km from estuary) is. Low water flow on the slope of 2-0%, and in this part of the river bed has a medium texture. The Babol and Babolsar Amir Kola Sewage that containing oil and oil directly fall out into the river. Babol slaughterhouse along with the excess ichors' is transported to the river. Pollution, chemical, mechanical and biological part due to its proximity to the estuary of migratory fish spawning and migration Babolrood has shattered. Area C: These parts of River are almost clear water and Rocky River bed and Length of about 44 km (64 km to estuary of). Biological reagents are presented in Map 2-1 percent slope and topography of the area is seen as low. Area D: This area within 64 kilometers long, about 30 km from the estuary of begins. Average slope of 5-2% of the area and the terrain is very variable. Water in this area is rich in oxygen. Flow (Debbie) of water in this region is very high. The savad-koh forests surrounding part of river is the. , And is located at an altitude of about 1500 m above sea level.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Chemical ; Mechanical ; Hydrology ; Hydrobiology ; Spawn ; Breeding ; Freshwater ; Brackishwater ; Pollution ; Migration ; Oxygen ; Water quality
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 142pp.
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  • 3
    Publication Date: 2021-05-19
    Description: The Regulatory Roadmap for Health and safety of fishery products is a strategy to produce a sustainable regulatory future that meets the objectives of protecting the Iranian public from the sale and advertising of unsafe fisheries health products, and supporting the safest consumption of fish and use of health fishery products. fishery The Roadmap provides the vision to transform nearly a dozen current frameworks for fish and health that are of various ages and regulatory approaches into an efficient, transparent, and aligned regulatory products health fisheriessystem that contributes directly to the safety of I ranian’s and the benefits they gain from fish and ll also be cognizant of the previous work accomplished by the Department, which As a strategy, it wi products. -health product or fish over its full life fisheriesrecognized that there is a progression in the knowl edge about a sociated with the benefits and harms of a product cycle. This increase in knowledge reduces the uncer tainty as As a consequence, it is logical to regulate in a and can lead to improved health outcomes for Irania n’s people. cycle.-manner that reflects the full life meaningful - re than ever, want transparency The Roadmap also acknowledges that Iranians, now mo Key to promoting meaningful transparency (beyond just access to information online) is taking transparency. providing leadership, being honest, taking action to engage s takeholders and the public, showcasing sources, and The ability of the regulator to gain and disseminate packaged) information.-good (not only new or pre information will be complemented by the obligations placed on regulated parties. With this vision and guiding principles, the National roadmap for health and safety of fishery products lays out the way to move from the old frameworks to the new regulatory system. This will require the sequencing of a number of amending initiatives, some staged in the near future and others implemented in the longer term. While aiming for the eventual comprehensive amendment of all regulatory frameworks for food and health products under the Food and Drugs Act, early emphasis will be placed upon amendments that will deliver: • The clearest value to Iranian’s and the health care and food safety systems. • The greatest efficiency by cutting through unsustainable administrative requirements or approaches and replacing them with ones drawing upon international partnering, best practices and new technological advantages to contribute directly to the safety of food and health products. Building upon these early initiatives, the resetting of the frameworks will be accomplished through a series of more comprehensive amendments over the next years. The benefit to those in the National roadmap for health and safety of fishery products is to have: • operational sustainability; • international alignment; and • the enabling of innovation and excellence in science to improve health outcomes. The National roadmap for health and safety of fishery products is structured into three parts: Where we are: provides an understanding of the existing regulatory frameworks (for fish and fisheries health products, including pharmaceuticals, natural fish health products, biologics, veterinary drugs, industrial devices) that are to be transformed. Where we are going: provides the objectives and an outline of the new regulatory system for implementation. How we will get there: guides discussion on the planning and sequencing of amendments over the next years, both short and long term, to best achieve modernization for the National roadmap for health and safety of fishery products.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fishery ; Roadmap ; Pharmaceuticals ; Biologic ; Veterinary drugs
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 39pp.
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  • 4
    Publication Date: 2021-05-19
    Description: In conformity with the sex determination of Gracilaria corticata in the Persian Gulf and Oman Sea, a total of 41 samples were collected from two stations of Bostaneh region (northern Persian Gulf, 54° 38´ E / 26° 30´ N) and Lipar regions (northern Oman Sea, 60° 49´ E / 25° 15´ N). The specimens were cultured in PES media for observing the different life stages. The anatomical structures of thallus were taken into consideration. The diploid tetrasporophytes and spermatangia in thallus of male’s gametophytes; and Carpospore and cystocarps of female were determined. Due to DNA extraction, the parasites and epiphytes were cleaned and then the under growing sections were sectioned using liquid nitrogen. After extraction of DNA, by using 20 different primers according to ISSR molecular indicator, the sex diversity and genetic diversity of populations were studied; and four primers were selected ultimately . The obtained results were analyzed by GenAlex and PopGen softwares. In total, 74 bands, all polymorphisms, were propagated. According to PIC index, polymorphism separation of primer C (0.33) was higher than other primers. The Marker Index was measured between 4.48 and 6.51 with mean Shannon’s index of 0.46. The genetic similarity amongst algae was 96%. The genetic diversity inter and intra populations had significant differences of which 83% of total diversity was related to the intra diversity and 17% was related to inter diversity populations. The highest genetic distance belonged to the specimens 5 (Bostaneh) and 35 (Lipar), and it indicated the inter populations diversity in addition to intra population. As an overall conclusion, these populations can be considered as broodstocks for hybrid production for further species breeding and also to attain the maximum heterosis in adaptation with environment. In Ward clustering analysis, the dendrograms showed 5 different clusters in genetic distance of 12.18 of isomorphic phases. The PCA analysis as a complementally method was used for attest the findings. In this research, the ISSR primers could determine the male and female gametophytes and diploid tetrasporophytes in which the primer A (bands of 1200 & 1700 bp) specific for diploid tetrasporophyte and band of 300 bp specific for male were produced. The primer C showed the bands of 820 & 900 bp for diploid tetrasporophyte, and 500 bp for female gametophyte. The primer AB (990 bp) for male, 520 bp for female and 1600 & 1900 bp for diploid tetrasporophyte were specified. The primer ABC showed the specific band of 1100 bp for male; 500 bp for female; and 1200 & 1500 bp for diploid tetrasporophytes.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sexual ; Diversity ; Red algae ; Generation ; Gracilaria corticata ; Samples ; DNA ; Population ; Broodstocks ; Environment ; Gametophyte ; Tetrasporophyte ; ISSR
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 108pp.
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  • 5
    Publication Date: 2021-05-19
    Description: To improvement the normative for better processing of Artemia urmiana cysts with Fluidized bed dryer method (FBD) and warm room drying system (W.R), a batch of Artemia cysts that harvested from urmia Lake was choose. Then 30 processing treatments include 6 different drying temperature (28.c, 30.c, 32.c, 34.c, 36.c) on 5 different process time (1, 2, 3, 4, 5, 6 hours) in warm room system and also 20 processing treatment include the same different internal temperatures of previous exams on 4 different process time (0. 5, 1, 1. 5, 2 hours) in fluidized bed dryer was applied. And after the same packing on All of 50 treatments, the desired factors like hatching percent and hatching efficiency and final water content of All treated samples estimated during 15 months and data registered and Analyzed with general Leaner model on spss program. According to results, the cysts samples that process statically with warm room system were better Hatching percent and Hatching efficiency Than Those That dried motional on fluidized bed dryer, also the best process treatment of FBD was the use of 28.c internal temperature for 0. 5 hour and the Best one of (W.R) was 34.c internal temperature for 4 hours. The (W.R) system had higher dry capacity than the FBD and also is cheaper to use. The Best remaining moisture of Artemia urmiana cysts to keep their permanent survive for at Least 15 months was estimated a bout %15.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Artemia urmiana ; Fluidized bed dryer warm room ; Cysts processing ; Hatching
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 56pp.
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    Publication Date: 2021-05-19
    Description: The concentration of serum immunoglobulin in rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)was measured by Immunoelectrophoresis. Serum total protein, also called plasma total protein or total protein, is a biochemical test for measuring the total amount of protein in blood plasma or serum. Protein in the plasma is made up of albumin and globulin. The globulin in turn is made up of α1, α2, β, and γ globulins. These fractions can be quantities using protein electrophoresis, but the total protein test is a faster and cheaper test that estimates the total of all fractions together. The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method, dye-binding and Refractometer are now available. The measurement is usually performed on automated analyzer salong with other laboratory tests. The normal IgM concentration was 3.3 mg ml in a group of free-living trout. While the IgM concentration was low in sera from fish living under aquarium conations. In visual variations were very pronounced. The purity of reference preparations and the specificity of anti sera used were examined by crossed Immunoelectrophoresis. Fish respond to antigenic stimulation by the production of immunoglobulin. So far only one immunoglobulin class is known to occur in teleosts, the characteristics of the class being rather similar to those of mammalian IgM. The molecule is a tetramers consisting of a basic structure of 8 light chains and 8 heavy chains (same molecular weight as the p-chains of mammals) (Acton et al. 1971, Etuis 1982). The molecular weight of the whole molecule is about 700 000 Daltons (13 to 16 S). Most studies concerning the humoral immune response in teleosts have dealt with aracteristics of the immune response elicited by known antigens. Only in a few cases has the concentration of total immunoglobulin been measured. Estimates of total immunoglobulin have been made in serum from (Oncorhynchus mykiss) and (Ctenopharyngodon idella) carp and goldfish (Vilain et al. 1984), carp (Richter et al. 1973), brown trout (Ingram & Alexander 1979) and certain salt-water fish (Fidler et al. 1969, Acton et al. 1971, Legler et al. 1971). Estimates of total IgM in serum from rainbow trout Salmo gairdnen have not, to our knowledge, been published so far.Serum. Blood samples were obtained by puncture of the caudal vein of rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)After clotting overnight at 4 "C the blood was centrifuged at 1000 g for 15 min to obtain serum. The sera were stored at -20 "C until examined. Total serum protein. Protein concentration in (Oncorhynchus mykiss) and(Ctenopharyngodon idella) sera was estimated by means of the Biuret method(Richtenich 1971). Antiserum to IgM : The monospecificity of the rabbit antiserum to rainbow trout IgM was indicated by the appearance of only one precipitation Line when the antiserum was reacted against normal trout serum in crossed Immunoelectrophoresis (Fig. 1). The purity of the IgM preparation which was used as reference IgM in connection with IgM quantification is illustrated in Fig. 2a. It appears that only one precipitation line developed when the preparation was reacted against antiserum to trout serum in crossed Immunoelectrophoresis. Fig. 2b illustrates the multi specificity of the rabbit antiserum to trout serum used.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biochemical ; Chemical ; Oncorhynchus mykiss ; Ctenopharyngodon idella ; Immunoglobulin ; Serum total protein ; Rainbow trout ; Plasma ; Blood ; Globulin ; Carp ; Goldfish
    Repository Name: AquaDocs
    Type: Report , Refereed
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  • 7
    Publication Date: 2021-05-19
    Description: Fish cell lines have been used to support many areas of research, beginning with fish viruses and extending into immunology, genetic studies, toxicology, environmental effects, aquaculture and seafood quality and it is the first step in the gene banking, to preserve gene materials. The present study we cultured cell of interior pituitary of the Acipenser gueldenstaedtii gland, as a first attempts in IRAN. FISH cell culture has widespread applications in virology, toxicology and as in vitro models in cytogenetic, biomedical, physiological researches. A cell line has been established from the Acipenser gueldenstaedtii interior pituitary and scales have been used to develop primary cell cultures. Recently, successful primary culture of the interior pituitary gland of Acipenser gueldenstaedtii has been developed by explants method. The present study evaluated the potential of several interior pituitary gland from different developmental stages for development of cell cultures using explants method. Pituitary gland from various stages of the adult Acipenser gueldenstaedtii was collected under standard aseptic conditions. Developing gonads from 15-20-year-old male and female sturgeon were collected during late April and early May2007. In all the cases the tissues were pooled in cold PBS antibiotic antimycotic solution (Sigma Chemicals, USA). The tissues were evaluated for attachment, growth and ability to undergo to produce suspension of cells. Primary cultures were initiated from the above tissues according to our earlier procedures, with certain modifications in the sub cultivation procedure. Briefly, tissues were cut into 1 mm3 size fragments which were seeded into 25 cm2 tissue culture flasks. After appropriate semidrying and addition of minimum essential medium (MEM) (Sigma, USA) supplemented with 15% fetal bovine serum (FBS) (Sigma, USA), cell growth was monitored. A seeding density of 1.5 105 cells as determined by a haemocytometer. The results have been showed the Acipenser gueldenstaedtii interior pituitary cells growth in incubator Co2 in 370 C, the cells adapted in this temperature. They were in Lag phase for 10 days, in log phase on 10- 22 days, and in stationary phase on 23- 28 days, after that they died. So we could produce sturgeon growth hormone from fish pituitary cells culture. By this study we can passage the cells on 21th day, for every week. In this way we can produce continued cell culture and store them for gene banking.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Acipenser gueldenstaedtii ; Pituitary gland ; Primary cell culture ; Fish
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 82pp.
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  • 8
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25377 | 18721 | 2018-09-14 07:42:27 | 25377 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The concentration of serum immunoglobulin in rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)was measured by Immunoelectrophoresis. Serum total protein, also called plasma total protein or total protein, is a biochemical test for measuring the total amount of protein in blood plasma or serum. Protein in the plasma is made up of albumin and globulin. The globulin in turn is made up of α1, α2, β, and γ globulins. These fractions can be quantities using protein electrophoresis, but the total protein test is a faster and cheaper test that estimates the total of all fractions together. The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method, dye-binding and Refractometer are now available. The measurement is usually performed on automated analyzer salong with other laboratory tests. The normal IgM concentration was 3.3 mg ml in a group of free-living trout. While the IgM concentration was low in sera from fish living under aquarium conations. In visual variations were very pronounced. The purity of reference preparations and the specificity of anti sera used were examined by crossed Immunoelectrophoresis. Fish respond to antigenic stimulation by the production of immunoglobulin. So far only one immunoglobulin class is known to occur in teleosts, the characteristics of the class being rather similar to those of mammalian IgM. The molecule is a tetramers consisting of a basic structure of 8 light chains and 8 heavy chains (same molecular weight as the p-chains of mammals) (Acton et al. 1971, Etuis 1982). The molecular weight of the whole molecule is about 700 000 Daltons (13 to 16 S). Most studies concerning the humoral immune response in teleosts have dealt with aracteristics of the immune response elicited by known antigens. Only in a few cases has the concentration of total immunoglobulin been measured. Estimates of total immunoglobulin have been made in serum from (Oncorhynchus mykiss) and (Ctenopharyngodon idella) carp and goldfish (Vilain et al. 1984), carp (Richter et al. 1973), brown trout (Ingram & Alexander 1979) and certain salt-water fish (Fidler et al. 1969, Acton et al. 1971, Legler et al. 1971). Estimates of total IgM in serum from rainbow trout Salmo gairdnen have not, to our knowledge, been published so far.Serum. Blood samples were obtained by puncture of the caudal vein of rainbow trout (Oncorhynchus mykiss) and(Ctenopharyngodon idella)After clotting overnight at 4 "C the blood was centrifuged at 1000 g for 15 min to obtain serum. The sera were stored at -20 "C until examined. Total serum protein. Protein concentration in (Oncorhynchus mykiss) and(Ctenopharyngodon idella) sera was estimated by means of the Biuret method(Richtenich 1971). Antiserum to IgM : The monospecificity of the rabbit antiserum to rainbow trout IgM was indicated by the appearance of only one precipitation Line when the antiserum was reacted against normal trout serum in crossed Immunoelectrophoresis (Fig. 1). The purity of the IgM preparation which was used as reference IgM in connection with IgM quantification is illustrated in Fig. 2a. It appears that only one precipitation line developed when the preparation was reacted against antiserum to trout serum in crossed Immunoelectrophoresis. Fig. 2b illustrates the multi specificity of the rabbit antiserum to trout serum used.
    Keywords: Health ; Iran ; Oncorhynchus mykiss ; Ctenopharyngodon idella ; Immunoglobulin ; Serum total protein ; Rainbow trout ; Plasma ; Blood ; Globulin ; Carp ; Goldfish
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 51
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  • 9
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    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25487 | 18721 | 2018-10-02 10:24:03 | 25487 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The Regulatory Roadmap for Health and safety of fishery products is a strategy to produce a sustainable regulatory future that meets the objectives of protecting the Iranian public from the sale and advertising of unsafe fisheries health products, and supporting the safest consumption of fish and use of health fishery products. fishery The Roadmap provides the vision to transform nearly a dozen current frameworks for fish and health that are of various ages and regulatory approaches into an efficient, transparent, and aligned regulatory products health fisheriessystem that contributes directly to the safety of I ranian’s and the benefits they gain from fish and ll also be cognizant of the previous work accomplished by the Department, which As a strategy, it wi products. -health product or fish over its full life fisheriesrecognized that there is a progression in the knowl edge about a sociated with the benefits and harms of a product cycle. This increase in knowledge reduces the uncer tainty as As a consequence, it is logical to regulate in a and can lead to improved health outcomes for Irania n’s people. cycle.-manner that reflects the full life meaningful than ever, want transparency The Roadmap also acknowledges that Iranians, now mo Key to promoting meaningful transparency (beyond just access to information online) is taking transparency. providing leadership, being honest, taking action to engage s takeholders and the public, showcasing sources, and The ability of the regulator to gain and disseminate packaged) information.-good (not only new or pre information will be complemented by the obligations placed on regulated parties. With this vision and guiding principles, the National roadmap for health and safety of fishery products lays out the way to move from the old frameworks to the new regulatory system. This will require the sequencing of a number of amending initiatives, some staged in the near future and others implemented in the longer term. While aiming for the eventual comprehensive amendment of all regulatory frameworks for food and health products under the Food and Drugs Act, early emphasis will be placed upon amendments that will deliver: The clearest value to Iranian’s and the health care and food safety systems. The greatest efficiency by cutting through unsustainable administrative requirements or approaches and replacing them with ones drawing upon international partnering, best practices and new technological advantages to contribute directly to the safety of food and health products. Building upon these early initiatives, the resetting of the frameworks will be accomplished through a series of more comprehensive amendments over the next years. The benefit to those in the National roadmap for health and safety of fishery products is to have: operational sustainability; international alignment; and the enabling of innovation and excellence in science to improve health outcomes. The National roadmap for health and safety of fishery products is structured into three parts: Where we are: provides an understanding of the existing regulatory frameworks (for fish and fisheries health products, including pharmaceuticals, natural fish health products, biologics, veterinary drugs, industrial devices) that are to be transformed. Where we are going: provides the objectives and an outline of the new regulatory system for implementation. How we will get there: guides discussion on the planning and sequencing of amendments over the next years, both short and long term, to best achieve modernization for the National roadmap for health and safety of fishery products.
    Keywords: Aquaculture ; Iran ; Fishery ; Roadmap ; Pharmaceuticals ; Biologic ; Veterinary drugs
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 39
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  • 10
    facet.materialart.
    Unknown
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25177 | 18721 | 2018-09-03 17:18:50 | 25177 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Fish cell lines have been used to support many areas of research, beginning with fish viruses and extending into immunology, genetic studies, toxicology, environmental effects, aquaculture and seafood quality and it is the first step in the gene banking, to preserve gene materials. The present study we cultured cell of interior pituitary of the Acipenser gueldenstaedtii gland, as a first attempts in IRAN. FISH cell culture has widespread applications in virology, toxicology and as in vitro models in cytogenetic, biomedical, physiological researches. A cell line has been established from the Acipenser gueldenstaedtii interior pituitary and scales have been used to develop primary cell cultures. Recently, successful primary culture of the interior pituitary gland of Acipenser gueldenstaedtii has been developed by explants method. The present study evaluated the potential of several interior pituitary gland from different developmental stages for development of cell cultures using explants method. Pituitary gland from various stages of the adult Acipenser gueldenstaedtii was collected under standard aseptic conditions. Developing gonads from 15-20-year-old male and female sturgeon were collected during late April and early May2007. In all the cases the tissues were pooled in cold PBS antibiotic antimycotic solution (Sigma Chemicals, USA). The tissues were evaluated for attachment, growth and ability to undergo to produce suspension of cells. Primary cultures were initiated from the above tissues according to our earlier procedures, with certain modifications in the sub cultivation procedure. Briefly, tissues were cut into 1 mm3 size fragments which were seeded into 25 cm2 tissue culture flasks. After appropriate semidrying and addition of minimum essential medium (MEM) (Sigma, USA) supplemented with 15% fetal bovine serum (FBS) (Sigma, USA), cell growth was monitored. A seeding density of 1.5 105 cells as determined by a hemocytometer. The results have been showed the Acipenser gueldenstaedtii interior pituitary cells growth in incubator Co2 in 370 C, the cells adapted in this temperature. They were in Lag phase for 10 days, in log phase on 10- 22 days, and in stationary phase on 23- 28 days, after that they died. So we could produce sturgeon growth hormone from fish pituitary cells culture. By this study we can passage the cells on 21th day, for every week. In this way we can produce continued cell culture and store them for gene banking.
    Keywords: Biology ; Iran ; Acipenser gueldenstaedtii ; Pituitary gland ; Primary cell culture ; Fish
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 82
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