ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary Giant protoplasts of Pleurotus cornucopiae were fused, using the glass microelectrode electrofusion technque; the percentage fusion achieved was 70%. To induce fusion, Ca2+ was necessary, a 10 mM concentration giving the best result. Polyethylene glycol 4000 (PEG) promoted fusion but also increased the adhesion of protoplasts, which caused them to be irreversibly attached to the electrodes. Fusion was always completed within 1 min after a single electrical pulse had been applied. The fused protoplast was isolated with a glass micropipette and was found to regenerate into a colony.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00250332
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