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  • 1
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    A study on immunological factors in White Spot Vaccinated shrimp, Litopenaeus vannamei in comparison to none vaccinated groups (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The aim of this study was to evaluate the efficacy of white spot virus vaccine produced by gamma iradiation in the face of Litopenaeus vannamei in comparison with Gracilaria corticata and Saccharomyces cerevisiae Seven hundred and twenty healthy shrimp SPF L. vannamei subadult with average weight of 10±1.02 g were collected and divided into 8 groups. The first group (T1) was fed with commercial pellet as control. The second group (T2) was fed with S. cerevisiae added to shrimp feed (1 g/Kg), the third group (T3) G. corticata so that algae Gracilaria were dried and added to shrimp feed at the rate of 1500 mg per kg and finally, the fourth group (T4) was vaccination group which the shrimp were exposed to the vaccine and injected intramuscularly gamma irradiant WSSV (1µl/gbw) for 10 days. The shrimps of all groups were then injected with WSSV and maintained for 25 days. Results indicated that the survival rates for groups T4, T3 T2 and T1 were 57.05±3.52%, 22.5±0.5%, 15±1.05% and 00.0±0%, respectively. Ultimately, at the end of the study the shrimp group T4 showed higher hematological data: THC, TPP, SOD, POD and PO. The study concluded that gamma irradiant WSSV is effective immunostimulants in shrimp L. vannamei and the immunity has better performances than those of the G. corticata and S. cerevisiae.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Gracilaria corticata ; Saccharomyces cerevisiae ; Litopenaeus vannamei ; Shrimp
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 54pp.
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  • 2
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    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25262 | 18721 | 2018-09-07 07:28:07 | 25262 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 than in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO_3, NO_2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Keywords: Aquaculture ; Iran ; Khouzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 78
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  • 3
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    A study on immunological factors in white spot vaccinated shrimp, Litopenaeus vannamei in comparison to none vaccinated groups (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25732 | 18721 | 2018-10-08 09:34:54 | 25732 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: The aim of this study was to evaluate the efficacy of white spot virus vaccine produced by gamma irradiation in the face of Litopenaeus vannamei in comparison with Gracilaria corticata and Saccharomyces cerevisiae Seven hundred and twenty healthy shrimp SPF L. vannamei subadult with average weight of 10±1.02 g were collected and divided into 8 groups. The first group (T1) was fed with commercial pellet as control. The second group (T2) was fed with S. cerevisiae added to shrimp feed (1 g/Kg), the third group (T3) G. corticata so that algae Gracilaria were dried and added to shrimp feed at the rate of 1500 mg per kg and finally, the fourth group (T4) was vaccination group which the shrimp were exposed to the vaccine and injected intramuscularly gamma irradiant WSSV (1µl/gbw) for 10 days. The shrimps of all groups were then injected with WSSV and maintained for 25 days. Results indicated that the survival rates for groups T4, T3 T2 and T1 were 57.05±3.52%, 22.5±0.5%, 15±1.05% and 00.0±0%, respectively. Ultimately, at the end of the study the shrimp group T4 showed higher hematological data: THC, TPP, SOD, POD and PO. The study concluded that gamma irradiant WSSV is effective immunostimulants in shrimp L. vannamei and the immunity has better performances than those of the G. corticata and S. cerevisiae.
    Keywords: Aquaculture ; Health ; Iran ; Gracilaria corticata ; Saccharomyces cerevisiae ; Litopenaeus vannamei ; Shrimp
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 54
    Location Call Number Expected Availability
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    PAPER (OA)
  • 4
    facet.materialart.
    Unknown
    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 then in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO3, NO2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Virology ; Histopathology ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
    Location Call Number Expected Availability
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