Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Physiologia plantarum
19 (1966), S. 0
ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
An attempt was made to isolate from potato tuber mitochondria the reported calcium-45 complex. Mitochondria fractionation results showed that the lipids were not involved in the calcium-45 complex formation. Using hot water as an extracting solvent it was found that two calcium-45 complexes occurred in the mitochondria fraction. One of the complexes was readily removable while the other complex was much less readily extractable with hot water. The readily removable calcinm-45 complex was identified as calcium phosphate and the less readily extractable complex seemed to be directly related to mitochondrial RNA liberation, however, it was impossible to isolate a calcium-45 ribonucleic acid complex. The extraction of the slowly removable calcium-45 complex was not a simple liberation of the complex but rather a breakdown which was irreversible and could be inhibited partially by sodium arsenate.A possible explanation of these data is that the TCA soluble fraction represents the total calcium45 uptake by the mitochondria, that is, the calcium-45 that has been accumulated plus that which is in the “calcium-carrier complex”. It is suggested that the easily extractable hot water fraction is the accumulated calicum-45 while the less readily extractable fraction is the “calcium-carrier complex”.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1966.tb07024.x
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