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  • Articles  (399)
  • Biology  (395)
  • Architecture, Civil Engineering, Surveying  (4)
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  • Articles  (399)
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  • 1
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    Role of βγ Subunits of G Proteins in Targeting the β-Adrenergic Receptor Kinase to Membrane-Bound Receptors (1992)
    American Association for the Advancement of Science
    Details
    Publication Date: 1992-08-28
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    A DIGITAL CONTROLLER FOR DISCRETE STAGE ACTIVATION OF AUTOMATIC PUMPING SAMPLERS (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 23 (1987), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : An electronic controller was developed to facilitate water sampling at discrete stage heights in perennial mountain streams of southeast Oklahoma. The controller operates in conjunction with an automatic pumping sampler and a magnetic, relay transducer. In response to changes in stage, the transducer generates an electrical pulse when relays are closed by a magnetic float. The controller monitors these relay closures and signals the sampler to pump a single sample at each desired water level during both rising and falling stages. After a sample is taken, additional switch closures at that stage axe ignored until the relay immediately above or below is closed. Inadvertent switch closures caused by turbulent stage fluctuations are not translated to the sampler thus eliminating duplicate or multiple samples at a given stage. Field testing has proven this device to be a reliable component with a range of applications for improving water quality sampling systems. The controller features battery operation, low power consumption, circuit simplicity and can be easily constructed for less than $100, including parts and labor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.1987.tb00813.x
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  • 3
    Electronic Resource
    Electronic Resource
    Influences on Release-Site Fidelity of Translocated Elk (2004)
    Oxford, UK; Malden , USA : Blackwell Science Inc
    Restoration ecology 12 (2004), S. 0 
    Details
    ISSN: 1526-100X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Several eastern states are considering the restoration of free-ranging elk populations via translocation from western populations. Optimal habitat immediately surrounding release sites has been found to enhance elk reintroduction success in western states. Little information exists, however, to aid eastern managers in identifying release sites with the highest chance of restoration success. We monitored the movements of 415 translocated elk released at three sites in southeastern Kentucky to identify landscape characteristics that enhance release-site fidelity. The distance elk moved after release differed among sites (F2,322 = 4.63, p = 0.01), age classes (F2,322 = 4.37, p = 0.01), and time intervals (F2,322 = 40.74, p 〈 0.001). At 6 and 12 months post-release, adults (15.81 ± 17.32 and 16.38 ± 20.29) and yearlings (13.91 ± 16.44 and 14.61 ± 21.11) moved farther than calves (8.06 ± 14.03 and 9.37 ± 14.40). The release site with the highest fidelity was privately owned, 15% open, and had the highest amount of edge compared with the other release sites. The two remaining sites contained large amounts of expansive openland or forest cover with lower amounts of edge. Additionally, both sites were publicly owned and experienced a higher degree of human-generated disturbance compared with the site to which elk were most faithful. When selecting release sites, managers should avoid areas dominated by a single cover type with little interspersion of other habitats. Rather, areas with high levels of open-forest edge (approximately 5.0 km/km2) and limited-human disturbance will likely enhance release-site fidelity and promote restoration success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1061-2971.2004.00231.x
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  • 4
    Electronic Resource
    Electronic Resource
    GABA and glycine may share the same conductance channel on cultured mammalian neurones (1979)
    [s.l.] : Nature Publishing Group
    Nature 277 (1979), S. 234-236 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 GABA and glycine activate channels with different conductances on the same spinal neurone grown in cell culture, a, Membrane current recordings from a neurone voltage clamped to -70 mV show inward current responses during iontophoresis of GABA (1,2) and glycine (3, 4). Iontophoresis duration ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/277234a0
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  • 5
    Electronic Resource
    Electronic Resource
    Benzodiazepines specifically modulate GABA-mediated postsynaptic inhibition in cultured mammalian neurones (1978)
    [s.l.] : Nature Publishing Group
    Nature 271 (1978), S. 563-564 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cultures were prepared from spinal cords removed from 13-14-d-old foetal mice as described previously19, and were grown on collagen-coated 35-mm dishes for 5-8 weeks before the study. Electrophysiological recordings were made on a modified stage of an inverted phase microscope at 37 C. All ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/271563a0
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  • 6
    Electronic Resource
    Electronic Resource
    Cellular immune responses persist and humoral responses decrease two decades after recovery from a single-source outbreak of hepatitis C (2000)
    [s.l.] : Nature America Inc.
    Nature medicine 6 (2000), S. 578-582 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] As acute hepatitis C virus (HCV) infection is clinically inapparent in most cases, the immunologic correlates of recovery are not well defined. The cellular immune response is thought to contribute to the elimination of HCV-infected cells and a strong HCV-specific T-helper-cell (Th) response is ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/75063
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleotide sequence, functional characterization and evolution of pFKN, a virulence plasmid in Pseudomonas syringae pathovar maculicola (2003)
    Oxford, UK : Blackwell Science, Ltd
    Molecular microbiology 47 (2003), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Pseudomonas syringae pv. maculicola strain M6 (Psm M6) carries the avrRpm1 gene, encoding a type III effector, on a 40 kb plasmid, pFKN. We hypothesized that this plasmid might carry additional genes required for pathogenesis on plants. We report the sequence and features of pFKN. In addition to avrRpm1, pFKN carries an allele of another type III effector, termed avrPphE, and a gene of unknown function (ORF8), expression of which is induced in planta, suggesting a role in the plant–pathogen interaction. The region of pFKN carrying avrRpm1, avrPphE and ORF8 exhibits several features of pathogenicity islands (PAIs). Curing of pFKN (creating Psm M6C) caused a significant reduction in virulence on Arabidopsis leaves. However, complementation studies using Psm M6C demonstrated an obvious virulence function only for avrRpm1. pFKN can integrate and excise from the chromosome of Psm M6 at low frequency via homologous recombination between identical sequence segments located on the chromosome and on pFKN. These segments are part of two nearly identical transposons carrying avrPphE. The avrPphE transposon was also detected in other strains of P. s. pv. maculicola and in P. s. tomato strain DC3000. The avrPphE transposon was found inserted at different loci in different strains. The analysis of sequences surrounding the avrPphE transposon insertion site in the chromosome of Psm M6 indicates that pFKN integrates into a PAI that encodes type III effectors. The integration of pFKN into this chromosomal region may therefore be seen as an evolutionary process determining the formation of a new PAI in the chromosome of Psm M6.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2003.03402.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mitochondrial uncoupling agents (1975)
    Springer
    The journal of membrane biology 25 (1975), S. 361-380 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Agents which uncouple oxidative phosphorylation in mitochondria were applied to identified neurons in an isolated ganglion of the marine molluscNavanax inermis. Aromatic monocarboxylic acids, acetanilides, benzamides, benzaldehydes and phenols all caused a rapid, reversible, dose-dependent increase in the membrane potential and conductance of the neurons tested. These events were due primarily to an increase in the membrane's conductance to potassium, relative to chloride. All active compounds also produced a reversible, dose-dependent decrease in the permeability of alkali-cations relative to potassium. The relative activity of congeners in each group of substances was directly correlated with the octanol-water partition coefficients of the various compounds, indicating that hydrophobicity was important in determining drug effect and suggesting that steric requirements were minimal. The results suggest that the observed changes in membrane electrical properties and cation selectivity are due to an increase in the membrane's anionic field strength caused by the hydrophobic interaction of anionic and nonionic agents with the neuronal membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868584
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of the parsley chalcone-synthase promoter in response to different light qualities (1994)
    Springer
    Planta 193 (1994), S. 275-282 
    Details
    ISSN: 1432-2048
    Keywords: Chalcone synthase ; Footprinting in vivo ; Gene expression (transient) ; Light regulation (UV-B photoreceptor, blue-light photoreceptor) ; Petroselinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the chalcone synthase (chs) promoter from parsley [Petroselinum crispum Miller (A.W. Hill)] for the existence of separate promoter elements responsible for transcriptional activation of the chs gene by UV-B and by blue light. A combination of in-vivo footprinting in parsley cells and light-induced transient expression assays with different chs promoter constructs in parsley protoplasts was used. Dark controls and bluelight-irradiated cells gave identical in-vivo footprints on the chs promoter. Pre-irradiation with blue light prior to a UV-B-light pulse is known to cause a shift in the timing of UV-B-light-induced increase in chs transcription rates. This shift was also manifested on the DNA template, since UV-B-light-induced in-vivo footprints in cells pretreated with blue light were detected earlier than in cells which had been irradiated with a UV-B-light pulse only. Although there was a clear shift in the timing of footprint appearance, the patterns of footprinting did not change. Light-induced transient-expression assays revealed that the shortest tested chs promoter which retained any light responsiveness, was sufficient for mediating both induction by UV light and the blue-light-mediated kinetic shift. These findings argue against a spatial separation of UV-B- and blue-light-responsive elements on the chs promoter. We interpret these data by postulating that the signal transduction pathways originating from the excitation of UV-B- and blue-light receptors merge at the chs promoter, or somewhere between light perception and protein-DNA interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192541
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  • 10
    Electronic Resource
    Electronic Resource
    A Ca-dependent Cl − conductance in cultured mouse spinal neurones (1984)
    [s.l.] : Nature Publishing Group
    Nature 311 (1984), S. 567-570 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Neurones were cultured from the embryonic mouse spinal cord according to methods published previously9. After 3-4 weeks in culture, we studied the cells at 23C with intracellular recording and two-electrode voltage-clamp techniques (Fig. 1). Most of our experiments used CsCl-filled microelectrodes ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/311567a0
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