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  • 1
    ISSN: 1573-5109
    Keywords: chromosome ; hybrid ; meiosis ; Oryza ; wild rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To determine the genomic constitution of Oryza meridionalis Ng (2n=2x=24) and to estimate genomic affinity between Asian and Australian wild species of rice containing the AA genomes, chromosome pairing was analyzed at metaphase I in O. meridionalis, O. rufipogon Griff. (2n=2x=24), O. nivara Sharma et Shastry (2n=2x=24), O. sativa f. spontanea Rosch, and their artifical hybrids. The Oryza parental species and their F1 hybrids showed normal meiosis, but slightly reduced chiasma fequency was observed in the hybrids. It is concluded from the cytological analysis that (i) the Australian O. meridionalis contains the AA genome which has very high affinity to that in the Asian AA genome wild species of rice; (ii) chromosome structual changes, such as inversions and reciprocal translocations, have occurred in the genomes of the different species studied.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5109
    Keywords: fertility ; hybridization ; Oryza ; wild rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interspecific and intraspecific crosses of rice species were made involving the AA genome wild species Oryza meridionalis Ng from Australia, O. nivara Sharma et Shastry, O. rufipogon Griff, the weedy type O. sativa f. spontanea Rosch., and the cultigen O. sativa L. from Asia. Seed set and the number of hybrids obtained from both interspecific and intraspecific crosses were low and no significant differences were observed between the interspecific and intraspecific crosses. In the combination O. meridionalis × O. nivara and O. meridionalis × O. rufipogon, considerable differences in reciprocal crosses were observed, whereas higher seed set and more hybrids were obtained when O. meridionalis was used as the female parent. Pollen stainability and seed fertility of hybrids were very low, averaging between 1% and 9% in both interspecific and intraspecific combinations, except in the O. nivara intraspecific cross of 105386 × 106111, whose hybrid showed 83.0% pollen stainabi lity and 66.5% seed fertility.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-5109
    Keywords: Oryza glumaepatula ; wild rice ; hybrid ; chromosome ; meiotic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to assess affinity of the AA genome in different wild Oryza species from Asia, Australia, and South America, chromosome pairing was analyzed at metaphase-I of the F1 hybrids obtained from interspecific crosses among O. rufipogon Griff., O. nivara Sharma et Shastry, O. glumaepatula Steud., and O. meridionalis Ng, and the hybrids produced between different populations of the same species. Both intraspecific and interspecific hybrids showed normal meiosis with remarkably high chromosome pairing at metaphase-I, which was comparable with the meiotic pairing of their respective parental species. An average of higher than 23 chiasmata per pollen mother cell (PMC) was observed in all the intraspecific and interspecific hybrids, except for one O. glumaepatula × O. nivara hybrid which had an average of 22.6 chiasmata per PMC at metaphase-I. No other meiotic irregularities except for a few bridges and laggards were found in the hybrids. It is concluded from this cytological study that the AA genome is essentially identical in the four Oryza species, as well as in different populations of the same rice species. It is therefore not recommended to differentiate the genomic designation of AA genomes by adding superscripts for different species.
    Type of Medium: Electronic Resource
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