ISSN:
1432-072X
Keywords:
SOS response
;
Mutant ssb genes
;
Two-dimensional electrophoresis
;
uvrA Protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A previously undescribed mutation in the ssb gene, which codes for a major single strand DNA binding protein essential for DNA repelication, was mapped on the Escherichia coli Chromosome. Three ssb mutants were analyzed under parallel physiological conditions for the induction of SOS proteins (products of recA, uvrA, and an unknown gene), the production of mutants, the induction of lambda prophage, and sensitivity to DNA damaging agents. Two-dimensional electrophoretic techniques were used to quantitate changes in the rate of synthesis of proteins. The previously unpublished position of the uvrA gene-product in the two-dimensional matrix of E. coli proteins was described. These ssb strains exhibited varying sensitivities to ultraviolet irradiation and methylmethane sulfonate that correlated with the rate of constitutive synthesis of SOS proteins, spontaneous commitment to virulent growth of lambda lysogens, and elevation of endogenous mutation rates.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00413009
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