ISSN:
1573-0603
Keywords:
squamous cell carcinoma
;
fibroblast
;
immunomagnetic adsorption
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A commonly encountered problem in primary cultures of tumor specimens is overgrowth of fibroblastic cells. A method to remove fibroblasts from primary cultures of squamous cell carcinoma of the head and neck using immunomagnetic adsorption is described. A monoclonal antibody (BR2) was used for fibroblast depletion. BR2 stains human fibroblasts but does not stain human endothelial cells, human keratinocytes, or the squamous cell carcinoma lines, A-431 and NCI-H292. Eight primary cultures of head and neck tumor specimens were established. BR2-positive cells were removed from the suspension of cultured cells by immunomagnetic adsorption, which employs a magnetic iron oxide conjugated goat anti-mouse antibody as a secondary reagent. The BR2 positive cells became adherent to the surface of the culture dish which was placed over a magnet. BR2-negative cells remained in suspension and were collected for further culture or study. These BR2-negative cells were shown by cytokeratin staining to be epithelial cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02387282
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