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  • 1
    Electronic Resource
    Electronic Resource
    The cortical reaction in pig oocytes during in vivo and in vitro fertilization (1986)
    New York, NY : Wiley-Blackwell
    Gamete Research 13 (1986), S. 241-251 
    Details
    ISSN: 0148-7280
    Keywords: oocyte ; fertilization ; cortical granule ; pig ; calcium ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The structure of pig oocytes after in vivo and in vitro fertilization and following treatment with the ionophore A23187 with differing levels of calcium are described, with particular reference to the cortical granules.Fertilization in vivo and in vitro resulted in cortical granule exocytosis. Sperm penetration in vivo was more rapid than in vitro and resulted in the dispersal of the cortical granules' contents in the perivitelline space following exocytosis. The contents of the granules remained adjacent to the plasmalemma after in vitro fertilization and suboolemmar vesicles were less numerous than after in vivo fertilization. High calcium levels were necessary to induce the dispersal of the cortical granule contents following treatment with ionophore. The observations are discussed regarding their relevance to the blockage to polyspermy.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120130307
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in cortical granules during porcine oocyte maturation (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 11 (1985), S. 311-319 
    Details
    ISSN: 0148-7280
    Keywords: oocytes ; cortical granule ; pig ; ultrastructure ; maturation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The structure, number, and distribution of cortical granules in porcine oocytes during maturation induced by human chorionic gonadotrophin (HCG) are reported.The number of granules remained constant for 30 hr following HCG. Thereafter, there was an approximate doubling by 50 hr. At all times examined, a dark and light form were present. Up to 40 hr the latter predominated while at 50 hr there was a marked increase in the number of the former. Movement of cortical granules to the plasma membrane took place between 20 and 30 hr post-HCG. The changes in cortical granules are correlated with the capacity of the oocytes to undergo a block to polyspermy.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120110311
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    Paper (German National Licenses)
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