ISSN:
1573-5044
Keywords:
anther culture
;
Capsicum annuum L.
;
haploid
;
stomatal length
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The number of chloroplasts per guard cell pair and the stomatal length of seventeen anther-derived plants obtained from three pepper donor genotypes were recorded. The haploid plants showed significantly fewer chloroplasts and shorter stomatal length than the diploid plants. Although both characters were positively correlated with the ploidy level, chloroplast number resulted a more reliable method compared to stomatal length to distinguish haploid from diploid in vitro-grown plantlets. The stomatal length values showed either significant variation among androgenic plants with the same ploidy or overlapping values between haploid and diploid individuals. On the contrary, the values for chloroplast number were more stable permitting discrimination of haploid from diploid plantlets regardless of their genetic background. Early ploidy estimation based on chloroplast counts of in vitro-grown plantlets may hasten pepper breeding programs utilizing anther-derived doubled haploids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00051583
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