ISSN:
1573-0603
Keywords:
germplasm storage
;
cryopreservation
;
Zea mays
;
Dactylis glomerata
;
totipotency
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Described in detail is a technique for the cryopreservation, long-term storage, thawing, recovery, and regrowth of embryogenic suspension cultures of maize (Zea mays L.) With this system we have successfully preserved many different culture lines, and recovered living material after more than 2 yr storage in liquid nitrogen. The recovered tissue is not apparently different from the starting material. With minor variations, the technique has also been used forDactylis glomerata and other suspension culture cells, as well as callus cultures. Approaches for the cryopreservation of other plant parts, such as seeds, embryos and meristems are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01404444
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