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Nonlinear filtering in improving the image quality of confocal fluorescent images (1991)

Hänninen, Pekka ; Stelzer, Ernst H. K. ; Salo, Juha

Springer

Machine vision and applications 4 (1991), S. 243-253

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ISSN: 1432-1769

Keywords: fluorescence imaging ; confocal ; median filtering ; biomedical microscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Computer Science

Notes: Abstract A different way of processing confocally scanned fluorescence images is presented. Linear median hybrid methods and linear filtering methods are compared numerically with a conventionally processed artificial data set and with real confocal data. The use of linear median hybrid techniques reduces the time required for recording three-dimensional data sets with a confocal fluorescence microscope as well as the photo-damage to the biological sample. The implementation of a linear median algorithm on the hardware level of a confocal microscope is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01815301

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