ISSN:
1432-0983
Keywords:
Key words ts mutants
;
Fission yeast
;
cdc mutants
;
Functional homologues
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have developed a procedure using a bank of temperature-sensitive (ts) mutants of fission yeast to identify mutants which can be rescued by expression of a plasmid-borne gene of interest. The procedure has been used to identify new ts alleles of cdc2 and swi7/pol1, a ts mutant rescued by actin, and to identify a ts allele of cdc11 which can be rescued by combined mammalian Myc and Max expression. The procedure should also be useful as an alternative approach to identify genes in fission yeast which are functionally homologous to genes of interest from other organisms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050244
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