ISSN:
1573-4986
Keywords:
beta-galactosidase
;
Bacillus circulans
;
GalβpNP, p-nitrophenyl beta-D-galactopyranoside
;
GlcNAcβpNP, p-nitrophenyl N-acetyl-β-D-glucosaminide
;
GalNAcαpNP, p-nitrophenyl N-acetyl-α-D-galactosaminide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A gene encoding β-galactosidase from Bacillus circulans which had hydrolysis specificity for the β1-3 linkage was expressed in Escherichia coli. The β-galactosidase was purified from crude cell lysates of E. coli by column chromatographies on Resource Q and Sephacryl S-200 HR. The enzyme released galactose with high selectivity from oligosaccharides which had terminal β1-3 linked galactose residues. However it did not hydrolyse β1-4 linked galactooligosaccharides. Moreover, Galβ1-3GlcNAc, Galβ1-3GalNAc, and their p-nitrophenyl glycosides were regioselectively synthesized in 10–46% yield by the transglycosylation reaction using this enzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006916222187
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