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  • UV damage  (1)
  • chloroplasts  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Repair mechanisms of UV-induced DNA damage in soybean chloroplasts (1995)
    Springer
    Plant molecular biology 29 (1995), S. 1267-1277 
    Details
    ISSN: 1573-5028
    Keywords: DNA repair ; Glycine max ; plastome ; quantitative PCR ; suspension cells ; UV damage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to better understand the biochemical mechanisms of DNA metabolism in chloroplasts, repair of UV induced plastome damage in vivo was determined by exposure of soybean suspension cells to UV light and subsequent quantitation of the damage remaining in nuclear and chloroplast encoded genes with time by quantitative polymerase chain reaction (QPCR). The kinetics of damage rapir in the nuclear rbcS gene suggest that photoreactivation and dark mechanisms are active, while for the plastome encoded psbA gene only a light-dependent repair process was detected which is considerably slower than would be expected for photolyase-mediated photoreactivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020467
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  • 2
    Electronic Resource
    Electronic Resource
    Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max (1990)
    Springer
    Plant molecular biology 15 (1990), S. 457-464 
    Details
    ISSN: 1573-5028
    Keywords: chloroplasts ; DNA helicase ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019162
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    Paper (German National Licenses)
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