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  • 1
    ISSN: 1573-4919
    Keywords: rat ribosomes ; yeast ribosomes ; ribosome reconstitution ; ribosome disassembly ; dimethylmaleic anhydride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Modification of 60S ribosomal subunits from rat liver with dimethylmaleic anhydride (60 μol/ml) is accompanied by release of 35% of the protein. The acidic ribosomal proteins, as well as 9 basic proteins, are selectively liberated from the ribosomal subunits. Reconstitution of the protein-deficient particles with the corresponding split proteins is accompanied by substantial recovery of the original polyphenylalanine synthetic activity. The described reconstitution procedure can be used to investigate the roles played by the released proteins and the functional similarities of proteins from different sources. Hybrid reconstitution of residual ribosomal particles from rat liver or yeast with the corresponding heterologous split proteins produces subunits which have incorporated heterologous proteins but are inactive in polyphenylalanine synthesis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular and cellular biochemistry 86 (1989), S. 55-63 
    ISSN: 1573-4919
    Keywords: yeast ribosomes ; ribosome disassembly ; ribosome reconstitution ; dimethylmaleic anhydride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Yeast 60S ribosomal subunits have been dissociated by reversible modification with dimethylmaleic anhydride. Treatment with 40 μmol reagent/ml releases 35% of the protein, producing core particles inactive in polyphenylalanine synthesis, which are totally or highly deficient in 17 different proteins. This preparation of residual particles recovers 45% of the original activity upon incubation with the released proteins. The reconstituted particles can be isolated by centrifugation without loss of activity, having the protein composition of the original subunits.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2019-07-13
    Description: The International Space Station (ISS) represents the culmination of over two decades of unprecedented global human endeavors to conceive, design, build and operate a research laboratory in space. Uninterrupted human presence in space since the inception of the ISS has been made possible by an international fleet of space vehicles facilitating crew rotation, delivery of science experiments and replenishment of propellants and supplies. On-orbit propulsion systems on both ISS and Visiting Vehicles are essential to the continuous operation of the ISS. This paper compares the ISS visiting vehicle propulsion systems by providing an overview of key design drivers, operational considerations and performance characteristics. Despite their differences in design, functionality, and purpose, all visiting vehicles must adhere to a common set of interface requirements along with safety and operational requirements. This paper addresses a wide variety of methods for satisfying these requirements and mitigating credible hazards anticipated during the on-orbit life of propulsion systems, as well as the seamless integration necessary for the continued operation of the ISS.
    Keywords: Space Transportation and Safety
    Type: JSC-CN-28459 , 2013 AIAA Houston Annual Technical Symposium; May 17, 2013; Houston, TX; United States
    Format: application/pdf
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