ISSN:
1432-203X
Keywords:
Key words Microsatellites
;
PCR
;
Protoplast fusion
;
Somatic hybrids
;
Solanum tuberosum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide primers comprising 5′-anchored di- and tri-nucleotide repeat motifs. PCR products were separated by polyacrylamide gel electrophoresis and visualised by silver staining. An average of 30.31 bands per primer were generated in each parent and, on average, 8.19 of these bands were polymorphic. S. sanctae-rosae-specific bands were observed in the hybrid material as was evidence of deletions, substitutions and rearrangements. The relative merits of this technique for the analysis of somatic hybrid material are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050735
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