ISSN:
1573-4943
Keywords:
Tandem mass spectrometry
;
multiprotein complexes
;
SEQUEST
;
database searching
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Methods to identify proteins contained in mixtures are described. The approach uses microcolumn liquid chromatography and automated tandem mass spectrometry in conjunction with protein and nucleotide database searching algorithms. This approach is applied to the identification of proteins obtained by immunoprecipitation reactions, interaction with a GST protein fusion products and interaction with a macromolecular complex.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026365528484
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