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  • embryogenesis  (3)
  • Regeneration  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Plant regeneration from stem cortex expiant and protoplast cultures of Brassica juncea (mustard) (1992)
    Springer
    Plant cell reports 11 (1992), S. 614-617 
    Details
    ISSN: 1432-203X
    Keywords: Brassica juncea ; Expiants ; Protoplasts ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11–14% of the cells had divided, and about 0.002% produced 1–2 mm colonies within 6 weeks. Up to 7% of these colonies gave rise to shoots upon transfer to plant regeneration medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236384
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  • 2
    Electronic Resource
    Electronic Resource
    Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata (1998)
    Springer
    Plant cell reports 17 (1998), S. 183-188 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassica ; Mustard ; Transgenic ; Transformation ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30–50% of the explants producing GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots but at a lower frequency (1–2%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050375
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of donor plant age and inflorescence age on microspore culture of Brassica napus L. (1991)
    Springer
    Euphytica 58 (1991), S. 51-55 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; donor plant age ; embryogenesis ; haploid ; microspore culture ; microspore stage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of age of donor plants and age of inflorescence on embryogenesis in microspore culture of B. napus was examined. Microspores isolated from buds of older plants had a higher embryo yield than those of younger ones. The effect of the age of inflorescence showed a different pattern. In older plants, a higher embryogenesis response was observed in microspores isolated from buds of new inflorescences, while in young plants, microspores isolated from buds of old inflorescences showed high embryo yield. These different responses were considered to be attributable to a difference in the developmental stage of pollen at the time of microspore isolation. Our results indicated that microspores collected from older inflorescences and older plants have sufficient embryogenic potential when the optimum developmental stage of pollen was used. Frequency of embryo to plant conversion was influenced by the size of embryos subcultured, but not by donor plant age or the age of the inflorescence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035339
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  • 4
    Electronic Resource
    Electronic Resource
    Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli (1993)
    Springer
    Plant cell, tissue and organ culture 35 (1993), S. 121-129 
    Details
    ISSN: 1573-5044
    Keywords: abscisic acid ; Brassica oleracea ; embryogenesis ; microspore culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Desiccation tolerance of broccoli microspore-derived embryos was induced by exogenous application of abscisic acid (ABA). Embryos, which were desiccated to about 10% water content, were estimated for viability after rehydration. Survival was dependent on the ABA concentration and the development stage of embryo, but not on the length of exposure period to ABA or genotype. Cotyledonary stage embryos acquired the highest desiccation tolerance when treated with 1×10-4M ABA. Under this condition, on average 27–48% of the desiccated embryos could convert into plants. Embryos treated with 1×10-6M ABA or no ABA or earlier development-staged embryos, such as globular and heart stages, lost viability after desiccation. A one day exposure to ABA had the similar effect on the induction of desiccation tolerance as a 7-day treatment. The dried embryos maintained their ability of plant conversion after three months of storage under room conditions. The plants derived from the desiccated embryos were not different in the morphology or ploidy level from those from non-desiccated ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032961
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  • 5
    Electronic Resource
    Electronic Resource
    Production of haploids via anther culture in Brassica oleracea var. Italica (1983)
    Springer
    Euphytica 32 (1983), S. 151-159 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea var. italica ; broccoli ; anther culture ; embryo culture ; haploid ; embryogenesis ; organogenesis ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Culture of broccoli (cv. Green Mountain) anthers at elevated temperatures (e.g. 35°C for two days) prior to maintenance at 25°C stimulated microspore embryogenesis. Embryo yields were also increased by subjecting excised inflorescences to a short term, high temperature shock (i.e. 45°C for one hour followed by 40°C for three hours) prior to anther plating and by a ten-fold increase in the auxin level of the anther culture medium. Plants were regenerated from microspore-derived embryos either directly through embryo culture or through the induction of organogenesis in explants of embryos which failed to develop on embryo culture medium. Approximately 50% of the regenerates were haploids with the remainder considered to be spontaneously-doubled haploids. Haploids were cloned and maintained as axial bud cultures for three years without the occurrence of ploidy changes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036875
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