ISSN:
1432-1327
Schlagwort(e):
Key words Azurin
;
Copper-A
;
Cyclic voltammetry
;
Protein folding
;
Rack-induced coordination
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract Cyclic voltammetry has been used to determine the reduction potentials of blue (Pseudomonas aeruginosa azurin) and purple (Thermus thermophilus CuA domain) copper proteins unfolded by guanidine hydrochloride. These Cu(II/I) potentials [456 (azurin); 453 (CuA) mV vs., NHE] are higher than those of the folded proteins. The downshift of the potential in the folded state can be accounted for by assuming that rack-induced axial coordination stabilizes Cu(II) relative to Cu(I) in a protein-encapsulated active site.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s007750050246
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