ISSN:
1432-2048
Keywords:
Chlorophenol red
;
Phosphinothricin acetyl-transferase
;
Protoplast
;
Transformation (selection)
;
Zea (transformed protoplasts)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Phosphinothricin (PPT) inhibits glutamine synthetase in plant cells, resulting in an accumulation of ammonia that can be toxic. Bar, from Streptomyces hygroscopicus is a gene that codes for phosphinothricin acetyl-transferase, an enzyme that detoxifies PPT by acetylation. We used polyethylene-glycol-mediated transformation to introduce bar into protoplasts of maize (Zea mays L.). With a novel assay utilizing the pH indicator chlorophenol red we identified transformants after only two to four weeks of selection on PPT.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00224783
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