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  • 1
    Electronic Resource
    Electronic Resource
    Specificity of strain and genotype in the susceptibility of pea to Agrobacterium tumefaciens (1989)
    Springer
    Plant cell reports 8 (1989), S. 274-277 
    Details
    ISSN: 1432-203X
    Keywords: Pisum sativum L. ; Transformation ; T-DNA ; Opines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine the best combination for potential use in transformation of Pisum sativum L., 13 genotypes were inoculated with wild-type Agrobacterium tumefaciens strains A281, C58 and Ach5. A281 appeared to be the most virulent strain, as determined by size and number of tumours, followed by C58 and Ach5. Genotypes differed considerably in their response to inoculation and genotype x strain interaction was evident. Genotypes also responded differently to in vivo or in vitro inoculation. Axenic calli from tumours could be grown on hormone-free medium and the presence of the specific opines for each strain in the callus indicated successful transfer and expression of T-DNA. Southern blot analysis of DNA from callus of A281-inoculated material showed that both TR and TL T-DNA had been incorporated into the pea genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274128
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  • 2
    Electronic Resource
    Electronic Resource
    Cold-sensitive mutation in Neurospora crassa affecting the production of 17S ribosomal RNA from ribosomal precursor RNA (1981)
    Springer
    Current genetics 4 (1981), S. 1-5 
    Details
    ISSN: 1432-0983
    Keywords: Neurospora crassa ; Ribosomal RNA processing ; Cold sensitive ; Ribosome ; Biosynthesis mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive temperature and vice versa. The results showed that the primary cold-sensitive defect in crib-1 is in the function, rather than the synthesis, of a molecule needed for the production of the 17S rRNA component of the 37S subunit. Pulse-labeling experiments showed that at 10 °C crib-1 synthesizes the 2.4-Mdal pre-rRNA molecule which contains the sequences for the 17S, 5.8S and 25S rRNA species, but that processing of this molecule is defective such that relatively few stable 17S rRNA molecules accumulate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376778
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