Publication Date:
2012-09-08
Description:
Meiotic recombination in budding yeast requires two RecA-related proteins, Rad51 and Dmc1, both of which form filaments on DNA capable of directing homology search and catalyzing formation of homologous joint molecules (JMs) and strand exchange. With use of a separation-of-function mutant form of Rad51 that retains filament-forming but not JM-forming activity, we show that the JM activity of Rad51 is fully dispensable for meiotic recombination. The corresponding mutation in Dmc1 causes a profound recombination defect, demonstrating Dmc1's JM activity alone is responsible for meiotic recombination. We further provide biochemical evidence that Rad51 acts with Mei5-Sae3 as a Dmc1 accessory factor. Thus, Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc1, during meiosis.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056682/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4056682/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cloud, Veronica -- Chan, Yuen-Ling -- Grubb, Jennifer -- Budke, Brian -- Bishop, Douglas K -- GM50936/GM/NIGMS NIH HHS/ -- R01 GM050936/GM/NIGMS NIH HHS/ -- T32 GM007197/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2012 Sep 7;337(6099):1222-5. doi: 10.1126/science.1219379.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Committee on Genetics, University of Chicago, Cummings Life Science Center, 920 East 58th Street, Chicago, IL 60637, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22955832" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Cycle Proteins/chemistry/*metabolism
;
Chromosomal Proteins, Non-Histone/metabolism
;
DNA, Fungal/chemistry/genetics/metabolism
;
DNA, Single-Stranded/chemistry/metabolism
;
DNA-Binding Proteins/chemistry/*metabolism
;
*Meiosis
;
Models, Molecular
;
Mutant Proteins/chemistry/metabolism
;
Nucleic Acid Conformation
;
Protein Binding
;
Rad51 Recombinase/chemistry/genetics/*metabolism
;
Recombinases/metabolism
;
*Recombination, Genetic
;
Saccharomyces cerevisiae/genetics/*physiology
;
Saccharomyces cerevisiae Proteins/chemistry/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink
